TY  - GEN
T1  - Cholesteryl ester transfer protein (CETP) is an important regulator to affect fatty liver in CETP transgenic mice
A1  - 王朝鐘
A2  - 王朝鐘
LA  - zh-TW
YR  - 2009
UL  - http://buscaintegrada.pucsp.br/vufind/Record/NDLTD-TW-097CSMU5107036
AB  - 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 97 ===   Cholesteryl Ester Transfer Protein (CETP) is a hydrophobic glycoprotein, secreted from the liver and circulates in blood stream, and mediates  the  transfer  of cholesteryl esters  from HDL  to  apo  B-containing  lipoproteins  in exchange  for  triglycerides. It is known that CETP induce atherosclerosis by decrease high density lipoprotein (HDL) level, and HDL is a factor for dyslipidemia in fatty liver disease patient, so, we predict that CETP may also be an important factor to induce fatty liver. Fatty liver is the most common chronic liver disease that may progress to hepatocellular carcinoma. Also, fatty liver disease frequently coexists with dyslipidemia, which is known as the decrease of HDL concentrations and other changes in patients’ lipid metabolism. Here we have introduced a relative new strategy for raising HDL cholesterol by inhibiting the level of CETP. The CETP level of CETP transgenic (CETP-Tg) mice feeding with high fat high cholesterol diet is markedly increased, then treating them with linear array epitope (LAE)-based anti-CETP vaccine, to induce CETP antibody. We found that the plasma CETP is markedly increased in CETP-Tg mice fed with high fat high cholesterol diet than in normal diet control. Using ELISA assay, we observed the titer of CETP antibody can be induced by LAE-based anti-CETP vaccine and after protein A/G pretreated serum antibody, the antibody-CETP complex is separated and detected by western blotting. In the blood analysis, HDL is slightly increased and alanine aminotransferase (ALT) is mild reduced in vaccinated CETP-Tg mice compare to non-vaccinated CETP-Tg mice. These data suggest that the anti-CETP vaccine have the potential to reduce CETP level, increase HDL level and decrease ALT in CETP-Tg mice plasma.


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