Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.

The Corneal limbus is a readily accessible region at the front of the eye, separating the cornea and sclera. Neural colonies (neurospheres) can be generated from adult corneal limbus in vitro. We have previously shown that these neurospheres originate from neural crest stem/progenitor cells and that...

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Autori principali: Xiaoli Chen, Heather Thomson, Jessica Cooke, Jennifer Scott, Parwez Hossain, Andrew Lotery
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Pubblicazione: Public Library of Science (PLoS) 2014-01-01
Accesso online:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0108418&type=printable
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spelling doaj-art-d04293fd3f424e35ae9a196ca3cf6bb12025-08-20T02:14:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e10841810.1371/journal.pone.0108418Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.Xiaoli ChenHeather ThomsonJessica CookeJennifer ScottParwez HossainAndrew LoteryThe Corneal limbus is a readily accessible region at the front of the eye, separating the cornea and sclera. Neural colonies (neurospheres) can be generated from adult corneal limbus in vitro. We have previously shown that these neurospheres originate from neural crest stem/progenitor cells and that they can differentiate into functional neurons in vitro. The aim of this study was to investigate whether mouse and human limbal neurosphere cells (LNS) could differentiate towards a retinal lineage both in vivo and in vitro following exposure to a developing retinal microenvironment. In this article we show that LNS can be generated from adult mice and aged humans (up to 97 years) using a serum free culture assay. Following culture with developing mouse retinal cells, we detected retinal progenitor cell markers, mature retinal/neuronal markers and sensory cilia in the majority of mouse LNS experiments. After transplantation into the sub-retinal space of neonatal mice, mouse LNS cells expressed photoreceptor specific markers, but no incorporation into host retinal tissue was seen. Human LNS cells also expressed retinal progenitor markers at the transcription level but mature retinal markers were not observed in vitro or in vivo. This data highlights that mouse corneal limbal stromal progenitor cells can transdifferentiate towards a retinal lineage. Complete differentiation is likely to require more comprehensive regulation; however, the accessibility and plasticity of LNS makes them an attractive cell resource for future study and ultimately therapeutic application.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0108418&type=printable
institution Directory of Open Access Journals
collection DOAJ
format Article
author Xiaoli Chen
Heather Thomson
Jessica Cooke
Jennifer Scott
Parwez Hossain
Andrew Lotery
spellingShingle Xiaoli Chen
Heather Thomson
Jessica Cooke
Jennifer Scott
Parwez Hossain
Andrew Lotery
Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.
PLoS ONE
10.1371/journal.pone.0108418
author_facet Xiaoli Chen
Heather Thomson
Jessica Cooke
Jennifer Scott
Parwez Hossain
Andrew Lotery
author_sort Xiaoli Chen
title Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.
title_short Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.
title_full Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.
title_fullStr Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.
title_full_unstemmed Adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.
title_sort adult limbal neurosphere cells: a potential autologous cell resource for retinal cell generation.
publisher Public Library of Science (PLoS)
publisher_facet Public Library of Science (PLoS)
container_title PLoS ONE
container_title_facet PLoS ONE
issn 1932-6203
publishDate 2014-01-01
container_volume 9
container_issue 10
container_startPage e108418
doi 10.1371/journal.pone.0108418
description The Corneal limbus is a readily accessible region at the front of the eye, separating the cornea and sclera. Neural colonies (neurospheres) can be generated from adult corneal limbus in vitro. We have previously shown that these neurospheres originate from neural crest stem/progenitor cells and that they can differentiate into functional neurons in vitro. The aim of this study was to investigate whether mouse and human limbal neurosphere cells (LNS) could differentiate towards a retinal lineage both in vivo and in vitro following exposure to a developing retinal microenvironment. In this article we show that LNS can be generated from adult mice and aged humans (up to 97 years) using a serum free culture assay. Following culture with developing mouse retinal cells, we detected retinal progenitor cell markers, mature retinal/neuronal markers and sensory cilia in the majority of mouse LNS experiments. After transplantation into the sub-retinal space of neonatal mice, mouse LNS cells expressed photoreceptor specific markers, but no incorporation into host retinal tissue was seen. Human LNS cells also expressed retinal progenitor markers at the transcription level but mature retinal markers were not observed in vitro or in vivo. This data highlights that mouse corneal limbal stromal progenitor cells can transdifferentiate towards a retinal lineage. Complete differentiation is likely to require more comprehensive regulation; however, the accessibility and plasticity of LNS makes them an attractive cell resource for future study and ultimately therapeutic application.
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0108418&type=printable
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AT jessicacooke adultlimbalneurospherecellsapotentialautologouscellresourceforretinalcellgeneration
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AT parwezhossain adultlimbalneurospherecellsapotentialautologouscellresourceforretinalcellgeneration
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first_indexed 2023-03-26T07:24:28Z
last_indexed 2025-09-06T08:24:25Z
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