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Ordered catenation of sequence-tagged sites and multiplexed SNP genotyping by sequencing
We describe a method for the efficient genotyping of SNPs, involving sequencing of ordered and catenated sequence-tagged sites (OCS). In OCS, short genomic segments, each containing an SNP, are amplified by PCR using primers that carry specially designed extra nucleotides at their 5′-ends. Amplifica...
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| Main Authors: | , |
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| Format: | Article |
| Language: | en |
| Published: |
Oxford University Press
2002
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC100310/ https://ncbi.nlm.nih.gov/pubmed/11809899 |
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