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Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line
Staphylococcus aureus frequently colonizes the airways of patients with compromised airway defenses (e.g., cystic fibrosis [CF] patients) for extended periods. Persistent and relapsing infections may be related to live S. aureus bacteria actively residing inside epithelial cells. In this study, we i...
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| المؤلفون الرئيسيون: | , , , , , , , |
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| التنسيق: | مقال |
| اللغة: | en |
| منشور في: |
American Society for Microbiology
2000
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.gov/pmc/articles/PMC101802/ https://ncbi.nlm.nih.gov/pubmed/10948168 |
| الوسوم: |
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pubmed-1018022002-09-12 Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line Kahl, Barbara C. Goulian, Mark van Wamel, Willem Herrmann, Mathias Simon, Sanford M. Kaplan, Gilla Peters, Georg Cheung, Ambrose L. Infect Immun Molecular and Cellular Pathogenesis Staphylococcus aureus frequently colonizes the airways of patients with compromised airway defenses (e.g., cystic fibrosis [CF] patients) for extended periods. Persistent and relapsing infections may be related to live S. aureus bacteria actively residing inside epithelial cells. In this study, we infected a respiratory epithelial cell line, which was derived from a CF patient, with S. aureus RN6390. Internalization of S. aureus was found to be time and dose dependent and could be blocked by cytochalasin D. Transmission electron microscopy revealed that internalized bacteria resided within endocytic vacuoles without any evidence of lysosomal fusion in a 24-h period. The results of internalization experiments and time-lapse fluorescence microscopy of epithelial cells infected with green fluorescent S. aureus indicate that, after an initial lag period of 7 to 9 h, intracellular bacteria began to replicate, with three to five divisions in a 24-h period, leading to apoptosis of infected cells. Induction of apoptosis required bacterial internalization and is associated with intracellular replication. The slow and gradual replication of S. aureus inside epithelial cells hints at the role of host factors or signals in bacterial growth and further suggests possible cross talk between host cells and S. aureus. American Society for Microbiology 2000-09 /pmc/articles/PMC101802/ /pubmed/10948168 Text en Copyright © 2000, American Society for Microbiology |
| institution |
US National Library of Medicine |
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PubMed Central |
| language |
en |
| format |
Article |
| topic |
Molecular and Cellular Pathogenesis |
| spellingShingle |
Molecular and Cellular Pathogenesis Kahl, Barbara C. Goulian, Mark van Wamel, Willem Herrmann, Mathias Simon, Sanford M. Kaplan, Gilla Peters, Georg Cheung, Ambrose L. Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line |
| description |
Staphylococcus aureus frequently colonizes the airways of patients with compromised airway defenses (e.g., cystic fibrosis [CF] patients) for extended periods. Persistent and relapsing infections may be related to live S. aureus bacteria actively residing inside epithelial cells. In this study, we infected a respiratory epithelial cell line, which was derived from a CF patient, with S. aureus RN6390. Internalization of S. aureus was found to be time and dose dependent and could be blocked by cytochalasin D. Transmission electron microscopy revealed that internalized bacteria resided within endocytic vacuoles without any evidence of lysosomal fusion in a 24-h period. The results of internalization experiments and time-lapse fluorescence microscopy of epithelial cells infected with green fluorescent S. aureus indicate that, after an initial lag period of 7 to 9 h, intracellular bacteria began to replicate, with three to five divisions in a 24-h period, leading to apoptosis of infected cells. Induction of apoptosis required bacterial internalization and is associated with intracellular replication. The slow and gradual replication of S. aureus inside epithelial cells hints at the role of host factors or signals in bacterial growth and further suggests possible cross talk between host cells and S. aureus. |
| author |
Kahl, Barbara C. Goulian, Mark van Wamel, Willem Herrmann, Mathias Simon, Sanford M. Kaplan, Gilla Peters, Georg Cheung, Ambrose L. |
| author_facet |
Kahl, Barbara C. Goulian, Mark van Wamel, Willem Herrmann, Mathias Simon, Sanford M. Kaplan, Gilla Peters, Georg Cheung, Ambrose L. |
| author_sort |
Kahl, Barbara C. |
| title |
Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line |
| title_short |
Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line |
| title_full |
Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line |
| title_fullStr |
Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line |
| title_full_unstemmed |
Staphylococcus aureus RN6390 Replicates and Induces Apoptosis in a Pulmonary Epithelial Cell Line |
| title_sort |
staphylococcus aureus rn6390 replicates and induces apoptosis in a pulmonary epithelial cell line |
| publisher |
American Society for Microbiology |
| publisher_facet |
American Society for Microbiology |
| publishDate |
2000 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC101802/ https://ncbi.nlm.nih.gov/pubmed/10948168 |
| _version_ |
1759030985386622976 |