Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60

A plasmid-encoded β-lactamase produced from a clinical strain of Providencia stuartii has been purified and characterized. The gene coding for the β-lactamase was cloned and sequenced. It appears to be a new natural TEM-derived enzyme, named TEM-60. Point mutations (Q39K, L51P, E104K, and R164S) are...

Descripció completa

Guardat en:
Dades bibliogràfiques
Autors principals: Franceschini, Nicola, Perilli, Mariagrazia, Segatore, Bernardetta, Setacci, Domenico, Amicosante, Gianfranco, Mazzariol, Annarita, Cornaglia, Giuseppe
Format: Article
Idioma:en
Publicat: American Society for Microbiology 1998
Matèries:
Mechanisms of Resistance
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC105622/
https://ncbi.nlm.nih.gov/pubmed/9624494
Etiquetes: Afegir etiqueta
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
id pubmed-105622
record_format dspace
spelling pubmed-1056222002-09-12 Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60 Franceschini, Nicola Perilli, Mariagrazia Segatore, Bernardetta Setacci, Domenico Amicosante, Gianfranco Mazzariol, Annarita Cornaglia, Giuseppe Antimicrob Agents Chemother Mechanisms of Resistance A plasmid-encoded β-lactamase produced from a clinical strain of Providencia stuartii has been purified and characterized. The gene coding for the β-lactamase was cloned and sequenced. It appears to be a new natural TEM-derived enzyme, named TEM-60. Point mutations (Q39K, L51P, E104K, and R164S) are present with respect to the TEM-1 enzyme; the mutation L51P has never been previously reported, with the exception of the chromosomally encoded extended-spectrum β-lactamase PER-1. Kinetic parameters relative to penicillins, cephalosporins, and monobactams other than mechanism-based inactivators were related to the in vitro susceptibility phenotype. American Society for Microbiology 1998-06 /pmc/articles/PMC105622/ /pubmed/9624494 Text en Copyright © 1998, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Mechanisms of Resistance
spellingShingle Mechanisms of Resistance
Franceschini, Nicola
Perilli, Mariagrazia
Segatore, Bernardetta
Setacci, Domenico
Amicosante, Gianfranco
Mazzariol, Annarita
Cornaglia, Giuseppe
Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60
description A plasmid-encoded β-lactamase produced from a clinical strain of Providencia stuartii has been purified and characterized. The gene coding for the β-lactamase was cloned and sequenced. It appears to be a new natural TEM-derived enzyme, named TEM-60. Point mutations (Q39K, L51P, E104K, and R164S) are present with respect to the TEM-1 enzyme; the mutation L51P has never been previously reported, with the exception of the chromosomally encoded extended-spectrum β-lactamase PER-1. Kinetic parameters relative to penicillins, cephalosporins, and monobactams other than mechanism-based inactivators were related to the in vitro susceptibility phenotype.
author Franceschini, Nicola
Perilli, Mariagrazia
Segatore, Bernardetta
Setacci, Domenico
Amicosante, Gianfranco
Mazzariol, Annarita
Cornaglia, Giuseppe
author_facet Franceschini, Nicola
Perilli, Mariagrazia
Segatore, Bernardetta
Setacci, Domenico
Amicosante, Gianfranco
Mazzariol, Annarita
Cornaglia, Giuseppe
author_sort Franceschini, Nicola
title Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60
title_short Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60
title_full Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60
title_fullStr Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60
title_full_unstemmed Ceftazidime and Aztreonam Resistance in Providencia stuartii: Characterization of a Natural TEM-Derived ExtendedSpectrum β-Lactamase, TEM-60
title_sort ceftazidime and aztreonam resistance in providencia stuartii: characterization of a natural tem-derived extendedspectrum β-lactamase, tem-60
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 1998
url https://ncbi.nlm.nih.gov/pmc/articles/PMC105622/
https://ncbi.nlm.nih.gov/pubmed/9624494
_version_ 1759031440457072640

Ítems similars