Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues

The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. The phytopathogen Rhodococcus fascians, a gram-positive organism, incites diverse developmental alterati...

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Autors principals: Vandeputte, Olivier, Öden, Sevgi, Mol, Adeline, Vereecke, Danny, Goethals, Koen, El Jaziri, Mondher, Prinsen, Els
Format: Article
Idioma:English
Publicat: American Society for Microbiology 2005
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Plant Microbiology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1065166/
https://ncbi.nlm.nih.gov/pubmed/15746315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1169-1177.2005
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spelling pubmed-10651662005-04-18 Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues Vandeputte, Olivier Öden, Sevgi Mol, Adeline Vereecke, Danny Goethals, Koen El Jaziri, Mondher Prinsen, Els Appl Environ Microbiol Plant Microbiology The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. The phytopathogen Rhodococcus fascians, a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. Phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. We show here for the first time that R. fascians produces and secretes the auxin indole-3-acetic acid. Interestingly, whereas noninfected-tobacco extracts have no effect, indole-3-acetic acid synthesis is highly induced in the presence of infected-tobacco extracts when tryptophan is not limiting. Indole-3-acetic acid production by a plasmid-free strain shows that the biosynthetic genes are located on the bacterial chromosome, although plasmid-encoded genes contribute to the kinetics and regulation of indole-3-acetic acid biosynthesis. The indole-3-acetic acid intermediates present in bacterial cells and secreted into the growth media show that the main biosynthetic route used by R. fascians is the indole-3-pyruvic acid pathway with a possible rate-limiting role for indole-3-ethanol. The relationship between indole-3-acetic acid production and the symptoms induced by R. fascians is discussed. American Society for Microbiology 2005-03 /pmc/articles/PMC1065166/ /pubmed/15746315 http://dx.doi.org/10.1128/AEM.71.3.1169-1177.2005 Text en Copyright © 2005, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Plant Microbiology
spellingShingle Plant Microbiology
Vandeputte, Olivier
Öden, Sevgi
Mol, Adeline
Vereecke, Danny
Goethals, Koen
El Jaziri, Mondher
Prinsen, Els
Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues
description The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little is known about indole-3-acetic acid biosynthesis and regulation in gram-positive bacteria. The phytopathogen Rhodococcus fascians, a gram-positive organism, incites diverse developmental alterations, such as leafy galls, on a wide range of plants. Phenotypic analysis of a leafy gall suggests that auxin may play an important role in the development of the symptoms. We show here for the first time that R. fascians produces and secretes the auxin indole-3-acetic acid. Interestingly, whereas noninfected-tobacco extracts have no effect, indole-3-acetic acid synthesis is highly induced in the presence of infected-tobacco extracts when tryptophan is not limiting. Indole-3-acetic acid production by a plasmid-free strain shows that the biosynthetic genes are located on the bacterial chromosome, although plasmid-encoded genes contribute to the kinetics and regulation of indole-3-acetic acid biosynthesis. The indole-3-acetic acid intermediates present in bacterial cells and secreted into the growth media show that the main biosynthetic route used by R. fascians is the indole-3-pyruvic acid pathway with a possible rate-limiting role for indole-3-ethanol. The relationship between indole-3-acetic acid production and the symptoms induced by R. fascians is discussed.
author Vandeputte, Olivier
Öden, Sevgi
Mol, Adeline
Vereecke, Danny
Goethals, Koen
El Jaziri, Mondher
Prinsen, Els
author_facet Vandeputte, Olivier
Öden, Sevgi
Mol, Adeline
Vereecke, Danny
Goethals, Koen
El Jaziri, Mondher
Prinsen, Els
author_sort Vandeputte, Olivier
title Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues
title_short Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues
title_full Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues
title_fullStr Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues
title_full_unstemmed Biosynthesis of Auxin by the Gram-Positive Phytopathogen Rhodococcus fascians Is Controlled by Compounds Specific to Infected Plant Tissues
title_sort biosynthesis of auxin by the gram-positive phytopathogen rhodococcus fascians is controlled by compounds specific to infected plant tissues
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2005
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1065166/
https://ncbi.nlm.nih.gov/pubmed/15746315
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.3.1169-1177.2005
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