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Role of rpoS in Stress Survival and Virulence of Vibrio cholerae
Vibrio cholerae is known to persist in aquatic environments under nutrient-limiting conditions. To analyze the possible involvement of the alternative sigma factor encoded by rpoS, which is shown to be important for survival during nutrient deprivation in several other bacterial species, a V. choler...
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American Society for Microbiology
1998
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC106954/ https://ncbi.nlm.nih.gov/pubmed/9473029 |
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pubmed-1069542002-09-13 Role of rpoS in Stress Survival and Virulence of Vibrio cholerae Yildiz, Fitnat H. Schoolnik, Gary K. J Bacteriol Physiology and Metabolism Vibrio cholerae is known to persist in aquatic environments under nutrient-limiting conditions. To analyze the possible involvement of the alternative sigma factor encoded by rpoS, which is shown to be important for survival during nutrient deprivation in several other bacterial species, a V. cholerae rpoS homolog was cloned by functional complementation of an Escherichia coli mutant by using a wild-type genomic library. Sequence analysis of the complementing clone revealed an 1.008-bp open reading frame which is predicted to encode a 336-amino-acid protein with 71 to 63% overall identity to other reported rpoS gene products. To determine the functional role of rpoS in V. cholerae, we inactivated rpoS by homologous recombination. V. cholerae strains lacking rpoS are impaired in the ability to survive diverse environmental stresses, including exposure to hydrogen peroxide, hyperosmolarity, and carbon starvation. These results suggest that rpoS may be required for the persistence of V. cholerae in aquatic habitats. In addition, the rpoS mutation led to reduced production or secretion of hemagglutinin/protease. However, rpoS is not critical for in vivo survival, as determined by an infant mouse intestinal competition assay. American Society for Microbiology 1998-02 /pmc/articles/PMC106954/ /pubmed/9473029 Text en Copyright © 1998, American Society for Microbiology |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
en |
| format |
Article |
| topic |
Physiology and Metabolism |
| spellingShingle |
Physiology and Metabolism Yildiz, Fitnat H. Schoolnik, Gary K. Role of rpoS in Stress Survival and Virulence of Vibrio cholerae |
| description |
Vibrio cholerae is known to persist in aquatic environments under nutrient-limiting conditions. To analyze the possible involvement of the alternative sigma factor encoded by rpoS, which is shown to be important for survival during nutrient deprivation in several other bacterial species, a V. cholerae rpoS homolog was cloned by functional complementation of an Escherichia coli mutant by using a wild-type genomic library. Sequence analysis of the complementing clone revealed an 1.008-bp open reading frame which is predicted to encode a 336-amino-acid protein with 71 to 63% overall identity to other reported rpoS gene products. To determine the functional role of rpoS in V. cholerae, we inactivated rpoS by homologous recombination. V. cholerae strains lacking rpoS are impaired in the ability to survive diverse environmental stresses, including exposure to hydrogen peroxide, hyperosmolarity, and carbon starvation. These results suggest that rpoS may be required for the persistence of V. cholerae in aquatic habitats. In addition, the rpoS mutation led to reduced production or secretion of hemagglutinin/protease. However, rpoS is not critical for in vivo survival, as determined by an infant mouse intestinal competition assay. |
| author |
Yildiz, Fitnat H. Schoolnik, Gary K. |
| author_facet |
Yildiz, Fitnat H. Schoolnik, Gary K. |
| author_sort |
Yildiz, Fitnat H. |
| title |
Role of rpoS in Stress Survival and Virulence of Vibrio cholerae |
| title_short |
Role of rpoS in Stress Survival and Virulence of Vibrio cholerae |
| title_full |
Role of rpoS in Stress Survival and Virulence of Vibrio cholerae |
| title_fullStr |
Role of rpoS in Stress Survival and Virulence of Vibrio cholerae |
| title_full_unstemmed |
Role of rpoS in Stress Survival and Virulence of Vibrio cholerae |
| title_sort |
role of rpos in stress survival and virulence of vibrio cholerae |
| publisher |
American Society for Microbiology |
| publisher_facet |
American Society for Microbiology |
| publishDate |
1998 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC106954/ https://ncbi.nlm.nih.gov/pubmed/9473029 |
| _version_ |
1759031627661443072 |