The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium

Thiamine pyrophosphate is an essential cofactor that is synthesized de novo in Salmonella typhimurium. The biochemical steps and gene products involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiami...

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Autori principali: Beck, Brian J., Downs, Diana M.
Natura: Articolo
Lingua:en
Pubblicazione: American Society for Microbiology 1998
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Physiology and Metabolism
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC106968/
https://ncbi.nlm.nih.gov/pubmed/9473043
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spelling pubmed-1069682002-09-13 The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium Beck, Brian J. Downs, Diana M. J Bacteriol Physiology and Metabolism Thiamine pyrophosphate is an essential cofactor that is synthesized de novo in Salmonella typhimurium. The biochemical steps and gene products involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiamine have yet to be elucidated. We have isolated mutations in a new locus (Escherichia coli open reading frame designation yojK) at 49 min on the S. typhimurium chromosome. Two significant phenotypes associated with lesions in this locus (apbE) were identified. First, apbE purF double mutants require thiamine, specifically the HMP moiety. Second, in the presence of adenine, apbE single mutants require thiamine, specifically both the HMP and the thiazole moieties. Together, the phenotypes associated with apbE mutants suggest that flux through the purine pathway has a role in regulating synthesis of the thiazole moiety of thiamine and are consistent with ApbE being involved in the conversion of AIR to HMP. The product of the apbE gene was found to be a 36-kDa membrane-associated lipoprotein, making it the second membrane protein implicated in thiamine synthesis. American Society for Microbiology 1998-02 /pmc/articles/PMC106968/ /pubmed/9473043 Text en Copyright © 1998, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Physiology and Metabolism
spellingShingle Physiology and Metabolism
Beck, Brian J.
Downs, Diana M.
The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium
description Thiamine pyrophosphate is an essential cofactor that is synthesized de novo in Salmonella typhimurium. The biochemical steps and gene products involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2-methyl pyrimidine (HMP) moiety of thiamine have yet to be elucidated. We have isolated mutations in a new locus (Escherichia coli open reading frame designation yojK) at 49 min on the S. typhimurium chromosome. Two significant phenotypes associated with lesions in this locus (apbE) were identified. First, apbE purF double mutants require thiamine, specifically the HMP moiety. Second, in the presence of adenine, apbE single mutants require thiamine, specifically both the HMP and the thiazole moieties. Together, the phenotypes associated with apbE mutants suggest that flux through the purine pathway has a role in regulating synthesis of the thiazole moiety of thiamine and are consistent with ApbE being involved in the conversion of AIR to HMP. The product of the apbE gene was found to be a 36-kDa membrane-associated lipoprotein, making it the second membrane protein implicated in thiamine synthesis.
author Beck, Brian J.
Downs, Diana M.
author_facet Beck, Brian J.
Downs, Diana M.
author_sort Beck, Brian J.
title The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium
title_short The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium
title_full The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium
title_fullStr The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium
title_full_unstemmed The apbE Gene Encodes a Lipoprotein Involved in Thiamine Synthesis in Salmonella typhimurium
title_sort apbe gene encodes a lipoprotein involved in thiamine synthesis in salmonella typhimurium
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 1998
url https://ncbi.nlm.nih.gov/pmc/articles/PMC106968/
https://ncbi.nlm.nih.gov/pubmed/9473043
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