A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development

Vacuolar processing enzyme (VPE) is a Cys proteinase responsible for the maturation of vacuolar proteins. Arabidopsis thaliana δVPE, which was recently found in the database, was specifically and transiently expressed in two cell layers of the seed coat (ii2 and ii3) at an early stage of seed develo...

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Main Authors: Nakaune, Satoru, Yamada, Kenji, Kondo, Maki, Kato, Tomohiko, Tabata, Satoshi, Nishimura, Mikio, Hara-Nishimura, Ikuko
Formato: Artigo
Idioma:English
Publicado em: American Society of Plant Biologists 2005
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Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1069705/
https://ncbi.nlm.nih.gov/pubmed/15705955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1105/tpc.104.026872
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spelling pubmed-10697052006-03-01 A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development Nakaune, Satoru Yamada, Kenji Kondo, Maki Kato, Tomohiko Tabata, Satoshi Nishimura, Mikio Hara-Nishimura, Ikuko Plant Cell Research Articles Vacuolar processing enzyme (VPE) is a Cys proteinase responsible for the maturation of vacuolar proteins. Arabidopsis thaliana δVPE, which was recently found in the database, was specifically and transiently expressed in two cell layers of the seed coat (ii2 and ii3) at an early stage of seed development. At this stage, cell death accompanying cell shrinkage occurs in the ii2 layer followed by cell death in the ii3 layer. In a δVPE-deficient mutant, cell death of the two layers of the seed coat was delayed. Immunocytochemical analysis localized δVPE to electron-dense structures inside and outside the walls of seed coat cells that undergo cell death. Interestingly, δVPE in the precipitate fraction from young siliques exhibits caspase-1–like activity, which has been detected in various types of plant cell death. Our results suggest that, at the early stage of seed development, δVPE is involved in cell death of limited cell layers, the purpose of which is to form a seed coat. American Society of Plant Biologists 2005-03 /pmc/articles/PMC1069705/ /pubmed/15705955 http://dx.doi.org/10.1105/tpc.104.026872 Text en Copyright © 2005, American Society of Plant Biologists
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Articles
spellingShingle Research Articles
Nakaune, Satoru
Yamada, Kenji
Kondo, Maki
Kato, Tomohiko
Tabata, Satoshi
Nishimura, Mikio
Hara-Nishimura, Ikuko
A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development
description Vacuolar processing enzyme (VPE) is a Cys proteinase responsible for the maturation of vacuolar proteins. Arabidopsis thaliana δVPE, which was recently found in the database, was specifically and transiently expressed in two cell layers of the seed coat (ii2 and ii3) at an early stage of seed development. At this stage, cell death accompanying cell shrinkage occurs in the ii2 layer followed by cell death in the ii3 layer. In a δVPE-deficient mutant, cell death of the two layers of the seed coat was delayed. Immunocytochemical analysis localized δVPE to electron-dense structures inside and outside the walls of seed coat cells that undergo cell death. Interestingly, δVPE in the precipitate fraction from young siliques exhibits caspase-1–like activity, which has been detected in various types of plant cell death. Our results suggest that, at the early stage of seed development, δVPE is involved in cell death of limited cell layers, the purpose of which is to form a seed coat.
author Nakaune, Satoru
Yamada, Kenji
Kondo, Maki
Kato, Tomohiko
Tabata, Satoshi
Nishimura, Mikio
Hara-Nishimura, Ikuko
author_facet Nakaune, Satoru
Yamada, Kenji
Kondo, Maki
Kato, Tomohiko
Tabata, Satoshi
Nishimura, Mikio
Hara-Nishimura, Ikuko
author_sort Nakaune, Satoru
title A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development
title_short A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development
title_full A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development
title_fullStr A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development
title_full_unstemmed A Vacuolar Processing Enzyme, δVPE, Is Involved in Seed Coat Formation at the Early Stage of Seed Development
title_sort vacuolar processing enzyme, δvpe, is involved in seed coat formation at the early stage of seed development
publisher American Society of Plant Biologists
publisher_facet American Society of Plant Biologists
publishDate 2005
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1069705/
https://ncbi.nlm.nih.gov/pubmed/15705955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1105/tpc.104.026872
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