Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi : Timing and Dose Dependency of Elicitor-Induced Reactions

Cultured parsley cells (Petroselinum crispum) responded to treatment with heat-released soluble cell-wall fragments (elicitors) from several different phytopathogenic fungi by forming coumarin derivatives (phytoalexins). This response was preceded in all cases by large but transient increases in the...

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Autors principals: Kombrink, Erich, Hahlbrock, Klaus
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Idioma:English
Publicat: 1986
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1075309/
https://ncbi.nlm.nih.gov/pubmed/16664778
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spelling pubmed-10753092005-04-11 Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi : Timing and Dose Dependency of Elicitor-Induced Reactions Kombrink, Erich Hahlbrock, Klaus Plant Physiol Articles Cultured parsley cells (Petroselinum crispum) responded to treatment with heat-released soluble cell-wall fragments (elicitors) from several different phytopathogenic fungi by forming coumarin derivatives (phytoalexins). This response was preceded in all cases by large but transient increases in the activities of two enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL). The activities of two hydrolytic enzymes, chitinase and 1,3-β-glucanase, also increased strongly in elicitor-treated cells, whereas the activities of three enzymes participating in primary metabolism were affected differently by the elicitor treatment. Glucose-6-phosphate dehydrogenase increased, phosphofructokinase remained almost constant, and pyrophosphate:fructose-6-phosphate phosphotransferase declined sharply in activity. Different amounts of cell-wall preparations from various phytopathogenic fungi were required for maximum elicitor activity. While three oomycetes (Phytophthora spp.) yielded the most active elicitors studied (maximum coumarin accumulation at concentrations of about 10 microgram per milliliter), cell-wall preparations from an ascomycete and three deuteromycetes gave comparable results only at 10 to 100 times higher concentrations. Optimal induction of PAL, 4CL, and chitinase with Phytophthora elicitor required only about 1 microgram per milliliter, whereas 1,3-β-glucanase induction showed a dose dependence similar to that observed for coumarins. The elicitor concentration had pronounced effects not only on the extent, but also on the timing of all induced reactions. 1986-05 /pmc/articles/PMC1075309/ /pubmed/16664778 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Articles
spellingShingle Articles
Kombrink, Erich
Hahlbrock, Klaus
Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi : Timing and Dose Dependency of Elicitor-Induced Reactions
description Cultured parsley cells (Petroselinum crispum) responded to treatment with heat-released soluble cell-wall fragments (elicitors) from several different phytopathogenic fungi by forming coumarin derivatives (phytoalexins). This response was preceded in all cases by large but transient increases in the activities of two enzymes of general phenylpropanoid metabolism, phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL). The activities of two hydrolytic enzymes, chitinase and 1,3-β-glucanase, also increased strongly in elicitor-treated cells, whereas the activities of three enzymes participating in primary metabolism were affected differently by the elicitor treatment. Glucose-6-phosphate dehydrogenase increased, phosphofructokinase remained almost constant, and pyrophosphate:fructose-6-phosphate phosphotransferase declined sharply in activity. Different amounts of cell-wall preparations from various phytopathogenic fungi were required for maximum elicitor activity. While three oomycetes (Phytophthora spp.) yielded the most active elicitors studied (maximum coumarin accumulation at concentrations of about 10 microgram per milliliter), cell-wall preparations from an ascomycete and three deuteromycetes gave comparable results only at 10 to 100 times higher concentrations. Optimal induction of PAL, 4CL, and chitinase with Phytophthora elicitor required only about 1 microgram per milliliter, whereas 1,3-β-glucanase induction showed a dose dependence similar to that observed for coumarins. The elicitor concentration had pronounced effects not only on the extent, but also on the timing of all induced reactions.
author Kombrink, Erich
Hahlbrock, Klaus
author_facet Kombrink, Erich
Hahlbrock, Klaus
author_sort Kombrink, Erich
title Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi : Timing and Dose Dependency of Elicitor-Induced Reactions
title_short Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi : Timing and Dose Dependency of Elicitor-Induced Reactions
title_full Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi : Timing and Dose Dependency of Elicitor-Induced Reactions
title_fullStr Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi : Timing and Dose Dependency of Elicitor-Induced Reactions
title_full_unstemmed Responses of Cultured Parsley Cells to Elicitors from Phytopathogenic Fungi : Timing and Dose Dependency of Elicitor-Induced Reactions
title_sort responses of cultured parsley cells to elicitors from phytopathogenic fungi : timing and dose dependency of elicitor-induced reactions
publishDate 1986
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1075309/
https://ncbi.nlm.nih.gov/pubmed/16664778
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