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An extrachromosomal tetracycline-regulatable system for mammalian cells
We have modified the tetracycline-regulatable system so that all components are present on a stable extrachromosomal vector that can replicate in a wide variety of mammalian cells. An EBV/human ori vector is used to carry the system, overcoming the species specificity of conventional Epstein–Barr vi...
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| Język: | en |
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Oxford University Press
2000
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC110717/ https://ncbi.nlm.nih.gov/pubmed/10954613 |
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pubmed-1107172002-08-15 An extrachromosomal tetracycline-regulatable system for mammalian cells Sclimenti, Christopher R. Baba, Edward J. Calos, Michele P. Nucleic Acids Res NAR Methods Online We have modified the tetracycline-regulatable system so that all components are present on a stable extrachromosomal vector that can replicate in a wide variety of mammalian cells. An EBV/human ori vector is used to carry the system, overcoming the species specificity of conventional Epstein–Barr virus vectors. By placing the transcriptional transactivator gene under autoregulation, better induction characteristics are obtained. This system offers greater speed and sensitivity than previously reported methods. It can be applied within 3–4 weeks and produces an induction range of several hundred-fold with a low background. Oxford University Press 2000-09-01 /pmc/articles/PMC110717/ /pubmed/10954613 Text en Copyright © 2000 Oxford University Press |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
en |
| format |
Article |
| topic |
NAR Methods Online |
| spellingShingle |
NAR Methods Online Sclimenti, Christopher R. Baba, Edward J. Calos, Michele P. An extrachromosomal tetracycline-regulatable system for mammalian cells |
| description |
We have modified the tetracycline-regulatable system so that all components are present on a stable extrachromosomal vector that can replicate in a wide variety of mammalian cells. An EBV/human ori vector is used to carry the system, overcoming the species specificity of conventional Epstein–Barr virus vectors. By placing the transcriptional transactivator gene under autoregulation, better induction characteristics are obtained. This system offers greater speed and sensitivity than previously reported methods. It can be applied within 3–4 weeks and produces an induction range of several hundred-fold with a low background. |
| author |
Sclimenti, Christopher R. Baba, Edward J. Calos, Michele P. |
| author_facet |
Sclimenti, Christopher R. Baba, Edward J. Calos, Michele P. |
| author_sort |
Sclimenti, Christopher R. |
| title |
An extrachromosomal tetracycline-regulatable system for mammalian cells |
| title_short |
An extrachromosomal tetracycline-regulatable system for mammalian cells |
| title_full |
An extrachromosomal tetracycline-regulatable system for mammalian cells |
| title_fullStr |
An extrachromosomal tetracycline-regulatable system for mammalian cells |
| title_full_unstemmed |
An extrachromosomal tetracycline-regulatable system for mammalian cells |
| title_sort |
extrachromosomal tetracycline-regulatable system for mammalian cells |
| publisher |
Oxford University Press |
| publisher_facet |
Oxford University Press |
| publishDate |
2000 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC110717/ https://ncbi.nlm.nih.gov/pubmed/10954613 |
| _version_ |
1759032179428425728 |