Multiepitopic B- and T-Cell Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine

We have attempted to develop an anti-human immunodeficiency virus (HIV) lipopeptide vaccine with several HIV-specific long peptides modified by C-terminal addition of a single palmitoyl chain. A mixture of six lipopeptides derived from regulatory or structural HIV-1 proteins (Nef, Gag, and Env) was...

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Main Authors: Gahéry-Ségard, Hanne, Pialoux, Gilles, Charmeteau, Bénédicte, Sermet, Sandrine, Poncelet, Hubert, Raux, Maurice, Tartar, André, Lévy, Jean-Paul, Gras-Masse, Helene, Guillet, Jean-Gérard
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2000
Assuntos:
Vaccines and Antiviral Agents
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC111644/
https://ncbi.nlm.nih.gov/pubmed/10644339
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spelling pubmed-1116442002-09-13 Multiepitopic B- and T-Cell Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine Gahéry-Ségard, Hanne Pialoux, Gilles Charmeteau, Bénédicte Sermet, Sandrine Poncelet, Hubert Raux, Maurice Tartar, André Lévy, Jean-Paul Gras-Masse, Helene Guillet, Jean-Gérard J Virol Vaccines and Antiviral Agents We have attempted to develop an anti-human immunodeficiency virus (HIV) lipopeptide vaccine with several HIV-specific long peptides modified by C-terminal addition of a single palmitoyl chain. A mixture of six lipopeptides derived from regulatory or structural HIV-1 proteins (Nef, Gag, and Env) was prepared. A phase I study was conducted to evaluate immunogenicity and tolerance in lipopeptide vaccination of HIV-1-seronegative volunteers given three injections of either 100, 250, or 500 μg of each lipopeptide, with or without immunoadjuvant (QS21). This report analyzes in detail B- and T-cell responses induced by vaccination. The lipopeptide vaccine elicited strong and multiepitopic B- and T-cell responses. Vaccinated subjects produced specific immunoglobulin G antibodies that recognized the Nef and Gag proteins. After the third injection, helper CD4(+)-T-cell responses as well as specific cytotoxic CD8(+) T cells were also obtained. These CD8(+) T cells were able to recognize naturally processed viral proteins. Finally, specific gamma interferon-secreting CD8(+) T cells were also detected ex vivo. American Society for Microbiology 2000-02 /pmc/articles/PMC111644/ /pubmed/10644339 Text en Copyright © 2000, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Vaccines and Antiviral Agents
spellingShingle Vaccines and Antiviral Agents
Gahéry-Ségard, Hanne
Pialoux, Gilles
Charmeteau, Bénédicte
Sermet, Sandrine
Poncelet, Hubert
Raux, Maurice
Tartar, André
Lévy, Jean-Paul
Gras-Masse, Helene
Guillet, Jean-Gérard
Multiepitopic B- and T-Cell Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine
description We have attempted to develop an anti-human immunodeficiency virus (HIV) lipopeptide vaccine with several HIV-specific long peptides modified by C-terminal addition of a single palmitoyl chain. A mixture of six lipopeptides derived from regulatory or structural HIV-1 proteins (Nef, Gag, and Env) was prepared. A phase I study was conducted to evaluate immunogenicity and tolerance in lipopeptide vaccination of HIV-1-seronegative volunteers given three injections of either 100, 250, or 500 μg of each lipopeptide, with or without immunoadjuvant (QS21). This report analyzes in detail B- and T-cell responses induced by vaccination. The lipopeptide vaccine elicited strong and multiepitopic B- and T-cell responses. Vaccinated subjects produced specific immunoglobulin G antibodies that recognized the Nef and Gag proteins. After the third injection, helper CD4(+)-T-cell responses as well as specific cytotoxic CD8(+) T cells were also obtained. These CD8(+) T cells were able to recognize naturally processed viral proteins. Finally, specific gamma interferon-secreting CD8(+) T cells were also detected ex vivo.
author Gahéry-Ségard, Hanne
Pialoux, Gilles
Charmeteau, Bénédicte
Sermet, Sandrine
Poncelet, Hubert
Raux, Maurice
Tartar, André
Lévy, Jean-Paul
Gras-Masse, Helene
Guillet, Jean-Gérard
author_facet Gahéry-Ségard, Hanne
Pialoux, Gilles
Charmeteau, Bénédicte
Sermet, Sandrine
Poncelet, Hubert
Raux, Maurice
Tartar, André
Lévy, Jean-Paul
Gras-Masse, Helene
Guillet, Jean-Gérard
author_sort Gahéry-Ségard, Hanne
title Multiepitopic B- and T-Cell Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine
title_short Multiepitopic B- and T-Cell Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine
title_full Multiepitopic B- and T-Cell Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine
title_fullStr Multiepitopic B- and T-Cell Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine
title_full_unstemmed Multiepitopic B- and T-Cell Responses Induced in Humans by a Human Immunodeficiency Virus Type 1 Lipopeptide Vaccine
title_sort multiepitopic b- and t-cell responses induced in humans by a human immunodeficiency virus type 1 lipopeptide vaccine
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2000
url https://ncbi.nlm.nih.gov/pmc/articles/PMC111644/
https://ncbi.nlm.nih.gov/pubmed/10644339
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