Purification and characterization of the imidazoleglycerol-phosphate dehydratase of Saccharomyces cerevisiae from recombinant Escherichia coli.

The HIS3+ gene of Saccharomyces cerevisiae was overexpressed in Escherichia coli and the recombinant imidazoleglycerol-phosphate dehydratase (IGPD) purified to homogeneity. Laser-desorption and electrospray m.s. indicated a molecular ion within 2 units of that expected (23833.3) on the basis of the...

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Autores principales: Hawkes, T R, Thomas, P G, Edwards, L S, Rayner, S J, Wilkinson, K W, Rice, D W
Formato: Artículo
Lenguaje:English
Publicado: 1995
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1136533/
https://ncbi.nlm.nih.gov/pubmed/7887893
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https://ncbi.nlm.nih.gov/pmc/articles/PMC1136533/
https://ncbi.nlm.nih.gov/pubmed/7887893

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