Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.

A commercially available, purified preparation of avidin was found to comprise two polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as assessed by biotin overlays of protein blots. The Mr 15,500 polypeptide was found to differ from the Mr 18,000 polypeptide only in i...

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Autori principali: Hiller, Y, Gershoni, J M, Bayer, E A, Wilchek, M
Natura: Articolo
Lingua:English
Pubblicazione: 1987
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1148514/
https://ncbi.nlm.nih.gov/pubmed/3435435
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spelling pubmed-11485142005-09-07 Biotin binding to avidin. Oligosaccharide side chain not required for ligand association. Hiller, Y Gershoni, J M Bayer, E A Wilchek, M Biochem J Research Article A commercially available, purified preparation of avidin was found to comprise two polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as assessed by biotin overlays of protein blots. The Mr 15,500 polypeptide was found to differ from the Mr 18,000 polypeptide only in its sugar content. When the commercial preparation was applied to a concanavalin A affinity column, the glycosylated forms were retarded as expected, and homotypic nonglycosylated avidin tetramers which failed to bind selectively to the column were collected in the effluent. The biotin-binding properties of the nonglycosylated avidin were equivalent to those obtained for the native (glycosylated) avidin molecule, indicating that the oligosaccharide moiety is not essential for the binding activity. 1987-11-15 /pmc/articles/PMC1148514/ /pubmed/3435435 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Hiller, Y
Gershoni, J M
Bayer, E A
Wilchek, M
Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.
description A commercially available, purified preparation of avidin was found to comprise two polypeptide bands (Mr 18,000 and Mr 15,500 respectively). Both bands bound biotin as assessed by biotin overlays of protein blots. The Mr 15,500 polypeptide was found to differ from the Mr 18,000 polypeptide only in its sugar content. When the commercial preparation was applied to a concanavalin A affinity column, the glycosylated forms were retarded as expected, and homotypic nonglycosylated avidin tetramers which failed to bind selectively to the column were collected in the effluent. The biotin-binding properties of the nonglycosylated avidin were equivalent to those obtained for the native (glycosylated) avidin molecule, indicating that the oligosaccharide moiety is not essential for the binding activity.
author Hiller, Y
Gershoni, J M
Bayer, E A
Wilchek, M
author_facet Hiller, Y
Gershoni, J M
Bayer, E A
Wilchek, M
author_sort Hiller, Y
title Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.
title_short Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.
title_full Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.
title_fullStr Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.
title_full_unstemmed Biotin binding to avidin. Oligosaccharide side chain not required for ligand association.
title_sort biotin binding to avidin. oligosaccharide side chain not required for ligand association.
publishDate 1987
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1148514/
https://ncbi.nlm.nih.gov/pubmed/3435435
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