1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60).

Human promyelocytic leukaemia cells (HL-60) can be induced to differentiate into mature granulocytes in vitro by 1 alpha,25-dihydroxycholecalciferol [1 alpha,25(OH)2D3], the active form of cholecalciferol. The differentiation-associated properties, such as phagocytosis and C3 rosette formation, were...

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Main Authors: Tanaka, H, Abe, E, Miyaura, C, Kuribayashi, T, Konno, K, Nishii, Y, Suda, T
Format: Article
Language:English
Published: 1982
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1158411/
https://ncbi.nlm.nih.gov/pubmed/6289803
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spelling pubmed-11584112005-09-07 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60). Tanaka, H Abe, E Miyaura, C Kuribayashi, T Konno, K Nishii, Y Suda, T Biochem J Research Article Human promyelocytic leukaemia cells (HL-60) can be induced to differentiate into mature granulocytes in vitro by 1 alpha,25-dihydroxycholecalciferol [1 alpha,25(OH)2D3], the active form of cholecalciferol. The differentiation-associated properties, such as phagocytosis and C3 rosette formation, were induced by as little as 0.12 nM-1 alpha,25(OH)2D3, and, at 12 nM, about half of the cells exhibited differentiation on day 3 of incubation. Concomitantly the viable cell number was decreased to less than half of the control. Among various derivatives of cholecalciferol examined, 1 alpha,25(OH)2D3 and 1 alpha,24R-dihydroxycholecalciferol were the most potent in inducing differentiation, followed successively by 1 alpha,24S-dihydroxycholecalciferol, 1 alpha-hydroxycholecalciferol, 25-hydroxycholecalciferol and 24R,25-dihydroxycholecalciferol. A cytosol protein specifically bound to 1 alpha,25 (OH)2D3 was found in HL-60 cells. Its physical properties closely resembled those found in such target tissues as intestine and parathyroid glands. 1 alpha,25(OH)2D3 bound to the cytosol receptor was transferred quantitatively to the chromatin fraction. The specificity of various derivatives of cholecalciferol in inducing differentiation was well correlated with that of their association with the cytosol receptor. These results are compatible with the hypothesis that the active form of cholecalciferol induces differentiation of human myeloid leukaemia cells by a mechanism similar to that proposed for the classical concept of steroid hormone action. 1982-06-15 /pmc/articles/PMC1158411/ /pubmed/6289803 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Tanaka, H
Abe, E
Miyaura, C
Kuribayashi, T
Konno, K
Nishii, Y
Suda, T
1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60).
description Human promyelocytic leukaemia cells (HL-60) can be induced to differentiate into mature granulocytes in vitro by 1 alpha,25-dihydroxycholecalciferol [1 alpha,25(OH)2D3], the active form of cholecalciferol. The differentiation-associated properties, such as phagocytosis and C3 rosette formation, were induced by as little as 0.12 nM-1 alpha,25(OH)2D3, and, at 12 nM, about half of the cells exhibited differentiation on day 3 of incubation. Concomitantly the viable cell number was decreased to less than half of the control. Among various derivatives of cholecalciferol examined, 1 alpha,25(OH)2D3 and 1 alpha,24R-dihydroxycholecalciferol were the most potent in inducing differentiation, followed successively by 1 alpha,24S-dihydroxycholecalciferol, 1 alpha-hydroxycholecalciferol, 25-hydroxycholecalciferol and 24R,25-dihydroxycholecalciferol. A cytosol protein specifically bound to 1 alpha,25 (OH)2D3 was found in HL-60 cells. Its physical properties closely resembled those found in such target tissues as intestine and parathyroid glands. 1 alpha,25(OH)2D3 bound to the cytosol receptor was transferred quantitatively to the chromatin fraction. The specificity of various derivatives of cholecalciferol in inducing differentiation was well correlated with that of their association with the cytosol receptor. These results are compatible with the hypothesis that the active form of cholecalciferol induces differentiation of human myeloid leukaemia cells by a mechanism similar to that proposed for the classical concept of steroid hormone action.
author Tanaka, H
Abe, E
Miyaura, C
Kuribayashi, T
Konno, K
Nishii, Y
Suda, T
author_facet Tanaka, H
Abe, E
Miyaura, C
Kuribayashi, T
Konno, K
Nishii, Y
Suda, T
author_sort Tanaka, H
title 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60).
title_short 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60).
title_full 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60).
title_fullStr 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60).
title_full_unstemmed 1 alpha,25-Dihydroxycholecalciferol and a human myeloid leukaemia cell line (HL-60).
title_sort 1 alpha,25-dihydroxycholecalciferol and a human myeloid leukaemia cell line (hl-60).
publishDate 1982
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1158411/
https://ncbi.nlm.nih.gov/pubmed/6289803
_version_ 1760273163689656320

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