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Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II
Genomic clones of Drosophila melanogaster were isolated from a λ library by cross-hybridization with the yeast gene coding for the 150-kd subunit of RNA polymerase II. Clones containing a region of ∼2.0 kb with strong homology to the yeast gene were shown to code for a 3.9-kb poly(A)(+)-RNA. Part of...
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| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
1986
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1166853/ https://ncbi.nlm.nih.gov/pubmed/16453680 |
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pubmed-11668532005-07-08 Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II Faust, Daniela M. Renkawitz-Pohl, Renate Falkenburg, Dieter Gasch, Alexander Bialojan, Siegfried Young, Richard A. Bautz, Ekkehard K. F. EMBO J Articles Genomic clones of Drosophila melanogaster were isolated from a λ library by cross-hybridization with the yeast gene coding for the 150-kd subunit of RNA polymerase II. Clones containing a region of ∼2.0 kb with strong homology to the yeast gene were shown to code for a 3.9-kb poly(A)(+)-RNA. Part of the coding region was cloned into an expression vector. A fusion protein was obtained which reacted with an antibody directed against RNA polymerase II of Drosophila. Peptide mapping of the fusion protein yielded a number of spots identical with spots derived from the 140-kd subunit of Drosophila RNA polymerase II. Sequence comparison of a segment of the Drosophila and the corresponding yeast clone yielded a high degree of homology at the protein level also, suggesting that we had isolated the gene coding for the 140-kd subunit of RNA polymerase II from Drosophila. In situ hybridization localized the DmRP140 gene at 88 A/B on chromosome 3 while the DmRP215 gene has previously been localized at 10 C on the X chromosome. Analysis of the transcripts (7.0 and 3.9 kb) in female and male flies shows dosage compensation for the transcription of the DmRP215 gene. 1986-04 /pmc/articles/PMC1166853/ /pubmed/16453680 Text en © IRL Press Limited, Oxford, England |
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US National Library of Medicine |
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PubMed Central |
| language |
English |
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Article |
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Articles |
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Articles Faust, Daniela M. Renkawitz-Pohl, Renate Falkenburg, Dieter Gasch, Alexander Bialojan, Siegfried Young, Richard A. Bautz, Ekkehard K. F. Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II |
| description |
Genomic clones of Drosophila melanogaster were isolated from a λ library by cross-hybridization with the yeast gene coding for the 150-kd subunit of RNA polymerase II. Clones containing a region of ∼2.0 kb with strong homology to the yeast gene were shown to code for a 3.9-kb poly(A)(+)-RNA. Part of the coding region was cloned into an expression vector. A fusion protein was obtained which reacted with an antibody directed against RNA polymerase II of Drosophila. Peptide mapping of the fusion protein yielded a number of spots identical with spots derived from the 140-kd subunit of Drosophila RNA polymerase II. Sequence comparison of a segment of the Drosophila and the corresponding yeast clone yielded a high degree of homology at the protein level also, suggesting that we had isolated the gene coding for the 140-kd subunit of RNA polymerase II from Drosophila. In situ hybridization localized the DmRP140 gene at 88 A/B on chromosome 3 while the DmRP215 gene has previously been localized at 10 C on the X chromosome. Analysis of the transcripts (7.0 and 3.9 kb) in female and male flies shows dosage compensation for the transcription of the DmRP215 gene. |
| author |
Faust, Daniela M. Renkawitz-Pohl, Renate Falkenburg, Dieter Gasch, Alexander Bialojan, Siegfried Young, Richard A. Bautz, Ekkehard K. F. |
| author_facet |
Faust, Daniela M. Renkawitz-Pohl, Renate Falkenburg, Dieter Gasch, Alexander Bialojan, Siegfried Young, Richard A. Bautz, Ekkehard K. F. |
| author_sort |
Faust, Daniela M. |
| title |
Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II |
| title_short |
Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II |
| title_full |
Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II |
| title_fullStr |
Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II |
| title_full_unstemmed |
Cloning and identification of the gene coding for the 140-kd subunit of Drosophila RNA polymerase II |
| title_sort |
cloning and identification of the gene coding for the 140-kd subunit of drosophila rna polymerase ii |
| publishDate |
1986 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC1166853/ https://ncbi.nlm.nih.gov/pubmed/16453680 |
| _version_ |
1760274202089226240 |