The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene.

The expression of Trypanosoma equiperdum variant surface protein (VSG) 78 is accomplished by the duplicative transposition of silent basic copy (BC) genes into a telomer-linked expression site to form an expression-linked copy (ELC). In two independent isolates expressing VSG 78, the ELC is a compos...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Main Authors: Roth, C W, Longacre, S, Raibaud, A, Baltz, T, Eisen, H
Formato: Artigo
Idioma:English
Publicado em: 1986
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1166902/
https://ncbi.nlm.nih.gov/pubmed/3013614
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!
id pubmed-1166902
record_format dspace
spelling pubmed-11669022005-07-08 The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene. Roth, C W Longacre, S Raibaud, A Baltz, T Eisen, H EMBO J Research Article The expression of Trypanosoma equiperdum variant surface protein (VSG) 78 is accomplished by the duplicative transposition of silent basic copy (BC) genes into a telomer-linked expression site to form an expression-linked copy (ELC). In two independent isolates expressing VSG 78, the ELC is a composite gene. The analysis of VSG 78 cDNA clones from these two Bo Tat 78 isolates and the respective BC genes revealed that both ELCs were constructed from the same three BC genes, a 3' BC which donated the last 255 bp of each ELC and two closely related 5' BCs. Although sequences of both 5' BC genes were found in each ELC, the junction with the 3' BC was provided by the same 5' BC in both cases. This 5' BC is an incomplete gene with insufficient open reading frame to code for a complete VSG and thus can only be used when joined to a competent 3' end. Furthermore, both 5' BC genes lack a conserved 14 nucleotide sequence found on all VSG mRNAs. These results support a model in which composite gene formation plays a role in the determination of the order of VSG expression. They also illustrate similarities between immunoglobulin gene and VSG gene construction. 1986-05 /pmc/articles/PMC1166902/ /pubmed/3013614 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Roth, C W
Longacre, S
Raibaud, A
Baltz, T
Eisen, H
The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene.
description The expression of Trypanosoma equiperdum variant surface protein (VSG) 78 is accomplished by the duplicative transposition of silent basic copy (BC) genes into a telomer-linked expression site to form an expression-linked copy (ELC). In two independent isolates expressing VSG 78, the ELC is a composite gene. The analysis of VSG 78 cDNA clones from these two Bo Tat 78 isolates and the respective BC genes revealed that both ELCs were constructed from the same three BC genes, a 3' BC which donated the last 255 bp of each ELC and two closely related 5' BCs. Although sequences of both 5' BC genes were found in each ELC, the junction with the 3' BC was provided by the same 5' BC in both cases. This 5' BC is an incomplete gene with insufficient open reading frame to code for a complete VSG and thus can only be used when joined to a competent 3' end. Furthermore, both 5' BC genes lack a conserved 14 nucleotide sequence found on all VSG mRNAs. These results support a model in which composite gene formation plays a role in the determination of the order of VSG expression. They also illustrate similarities between immunoglobulin gene and VSG gene construction.
author Roth, C W
Longacre, S
Raibaud, A
Baltz, T
Eisen, H
author_facet Roth, C W
Longacre, S
Raibaud, A
Baltz, T
Eisen, H
author_sort Roth, C W
title The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene.
title_short The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene.
title_full The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene.
title_fullStr The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene.
title_full_unstemmed The use of incomplete genes for the construction of a Trypanosoma equiperdum variant surface glycoprotein gene.
title_sort use of incomplete genes for the construction of a trypanosoma equiperdum variant surface glycoprotein gene.
publishDate 1986
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1166902/
https://ncbi.nlm.nih.gov/pubmed/3013614
_version_ 1760274208643874816

Registos relacionados