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Suppressors of the Ndc10-2 Mutation: A Role for the Ubiquitin System in Saccharomyces Cerevisiae Kinetochore Function
We have isolated a new conditional-lethal mutation, ndc10-2, in the NDC10/CBF2/CTF14 gene that encodes the 110-kD subunit of the Saccharomyces cerevisiae CBF3 kinetochore complex. At the restrictive temperature of 37°, ndc10-2 cells are able to assemble anaphase spindles, but fail to segregate their...
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| フォーマット: | 論文 |
| 言語: | English |
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1997
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| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1208167/ https://ncbi.nlm.nih.gov/pubmed/9335582 |
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pubmed-12081672006-09-13 Suppressors of the Ndc10-2 Mutation: A Role for the Ubiquitin System in Saccharomyces Cerevisiae Kinetochore Function Kopski, K. M. Huffaker, T. C. Genetics Investigations We have isolated a new conditional-lethal mutation, ndc10-2, in the NDC10/CBF2/CTF14 gene that encodes the 110-kD subunit of the Saccharomyces cerevisiae CBF3 kinetochore complex. At the restrictive temperature of 37°, ndc10-2 cells are able to assemble anaphase spindles, but fail to segregate their DNA, consistent with a defect in kinetochore function. To identify other factors that play a role in kinetochore assembly or function, we isolated both dosage and second site suppressors of the ndc10-2 mutation. These screens identified UBC6 as a dosage suppressor, and mutations in UBC6 and UBC7 as second-site suppressors of ndc10-2 heat sensitivity. Both UBC6 and UBC7 encode ubiquitin-conjugating enzymes that function in ubiquitin-mediated protein degradation. Furthermore, overexpression of a mutant ubiquitin suppresses the ndc10-2 mutation. These results implicate the ubiquitin system in the regulation of ndc10-2 function and suggest a role for the ubiquitin system in kinetochore function. 1997-10 /pmc/articles/PMC1208167/ /pubmed/9335582 Text en |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
English |
| format |
Article |
| topic |
Investigations |
| spellingShingle |
Investigations Kopski, K. M. Huffaker, T. C. Suppressors of the Ndc10-2 Mutation: A Role for the Ubiquitin System in Saccharomyces Cerevisiae Kinetochore Function |
| description |
We have isolated a new conditional-lethal mutation, ndc10-2, in the NDC10/CBF2/CTF14 gene that encodes the 110-kD subunit of the Saccharomyces cerevisiae CBF3 kinetochore complex. At the restrictive temperature of 37°, ndc10-2 cells are able to assemble anaphase spindles, but fail to segregate their DNA, consistent with a defect in kinetochore function. To identify other factors that play a role in kinetochore assembly or function, we isolated both dosage and second site suppressors of the ndc10-2 mutation. These screens identified UBC6 as a dosage suppressor, and mutations in UBC6 and UBC7 as second-site suppressors of ndc10-2 heat sensitivity. Both UBC6 and UBC7 encode ubiquitin-conjugating enzymes that function in ubiquitin-mediated protein degradation. Furthermore, overexpression of a mutant ubiquitin suppresses the ndc10-2 mutation. These results implicate the ubiquitin system in the regulation of ndc10-2 function and suggest a role for the ubiquitin system in kinetochore function. |
| author |
Kopski, K. M. Huffaker, T. C. |
| author_facet |
Kopski, K. M. Huffaker, T. C. |
| author_sort |
Kopski, K. M. |
| title |
Suppressors of the Ndc10-2 Mutation: A Role for the Ubiquitin System in Saccharomyces Cerevisiae Kinetochore Function |
| title_short |
Suppressors of the Ndc10-2 Mutation: A Role for the Ubiquitin System in Saccharomyces Cerevisiae Kinetochore Function |
| title_full |
Suppressors of the Ndc10-2 Mutation: A Role for the Ubiquitin System in Saccharomyces Cerevisiae Kinetochore Function |
| title_fullStr |
Suppressors of the Ndc10-2 Mutation: A Role for the Ubiquitin System in Saccharomyces Cerevisiae Kinetochore Function |
| title_full_unstemmed |
Suppressors of the Ndc10-2 Mutation: A Role for the Ubiquitin System in Saccharomyces Cerevisiae Kinetochore Function |
| title_sort |
suppressors of the ndc10-2 mutation: a role for the ubiquitin system in saccharomyces cerevisiae kinetochore function |
| publishDate |
1997 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC1208167/ https://ncbi.nlm.nih.gov/pubmed/9335582 |
| _version_ |
1760279786765156352 |