Ex vivo cell labeling with (64)Cu–pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography

We have used copper-64-pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) ((64)Cu–PTSM) to radiolabel cells ex vivo for in vivo positron-emission tomography (PET) imaging studies of cell trafficking in mice and for eventual application in patients. 2-[(18)F]-Fluoro-2-deoxy-d-glucose (FDG) cell labeling...

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Main Authors: Adonai, Nora, Nguyen, Khoi N., Walsh, Joseph, Iyer, M., Toyokuni, Tatsushi, Phelps, Michael E., McCarthy, Timothy, McCarthy, Deborah W., Gambhir, Sanjiv Sam
Formato: Artigo
Idioma:en
Publicado em: National Academy of Sciences 2002
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC122467/
https://ncbi.nlm.nih.gov/pubmed/11867752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052709599
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spelling pubmed-1224672002-09-12 Ex vivo cell labeling with (64)Cu–pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography Adonai, Nora Nguyen, Khoi N. Walsh, Joseph Iyer, M. Toyokuni, Tatsushi Phelps, Michael E. McCarthy, Timothy McCarthy, Deborah W. Gambhir, Sanjiv Sam Proc Natl Acad Sci U S A Biological Sciences We have used copper-64-pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) ((64)Cu–PTSM) to radiolabel cells ex vivo for in vivo positron-emission tomography (PET) imaging studies of cell trafficking in mice and for eventual application in patients. 2-[(18)F]-Fluoro-2-deoxy-d-glucose (FDG) cell labeling also was evaluated for comparison. (64)Cu–PTSM uptake by C6 rat glioma (C6) cells increased for 180 min and then stabilized. The labeling efficiency was directly proportional to (64)Cu–PTSM concentration and influenced negatively by serum. Label uptake per cell was greater with (64)Cu–PTSM than with FDG. However, both (64)Cu–PTSM- and FDG-labeled cells showed efflux of cell activity into supernatant. The (64)Cu–PTSM labeling procedure did not interfere significantly with C6 cell viability and proliferation rate. MicroPET images of living mice indicate that tail-vein-injected labeled C6 cells traffic to the lungs and liver. In addition, transient splenic accumulation of radioactivity was clearly detectable in a mouse scanned at 3.33 h postinfusion of (64)Cu–PTSM-labeled lymphocytes. In contrast, the liver was the principal organ of tracer localization after tail-vein administration of (64)Cu–PTSM alone. These results indicate that in vivo imaging of cell trafficking is possible with (64)Cu–PTSM-labeled cells. Given the longer t(1/2) of (64)Cu (12.7 h) relative to (18)F (110 min), longer cell-tracking periods (up to 24–36 h) should be possible now with PET. National Academy of Sciences 2002-03-05 2002-02-26 /pmc/articles/PMC122467/ /pubmed/11867752 http://dx.doi.org/10.1073/pnas.052709599 Text en Copyright © 2002, The National Academy of Sciences
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Biological Sciences
spellingShingle Biological Sciences
Adonai, Nora
Nguyen, Khoi N.
Walsh, Joseph
Iyer, M.
Toyokuni, Tatsushi
Phelps, Michael E.
McCarthy, Timothy
McCarthy, Deborah W.
Gambhir, Sanjiv Sam
Ex vivo cell labeling with (64)Cu–pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
description We have used copper-64-pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) ((64)Cu–PTSM) to radiolabel cells ex vivo for in vivo positron-emission tomography (PET) imaging studies of cell trafficking in mice and for eventual application in patients. 2-[(18)F]-Fluoro-2-deoxy-d-glucose (FDG) cell labeling also was evaluated for comparison. (64)Cu–PTSM uptake by C6 rat glioma (C6) cells increased for 180 min and then stabilized. The labeling efficiency was directly proportional to (64)Cu–PTSM concentration and influenced negatively by serum. Label uptake per cell was greater with (64)Cu–PTSM than with FDG. However, both (64)Cu–PTSM- and FDG-labeled cells showed efflux of cell activity into supernatant. The (64)Cu–PTSM labeling procedure did not interfere significantly with C6 cell viability and proliferation rate. MicroPET images of living mice indicate that tail-vein-injected labeled C6 cells traffic to the lungs and liver. In addition, transient splenic accumulation of radioactivity was clearly detectable in a mouse scanned at 3.33 h postinfusion of (64)Cu–PTSM-labeled lymphocytes. In contrast, the liver was the principal organ of tracer localization after tail-vein administration of (64)Cu–PTSM alone. These results indicate that in vivo imaging of cell trafficking is possible with (64)Cu–PTSM-labeled cells. Given the longer t(1/2) of (64)Cu (12.7 h) relative to (18)F (110 min), longer cell-tracking periods (up to 24–36 h) should be possible now with PET.
author Adonai, Nora
Nguyen, Khoi N.
Walsh, Joseph
Iyer, M.
Toyokuni, Tatsushi
Phelps, Michael E.
McCarthy, Timothy
McCarthy, Deborah W.
Gambhir, Sanjiv Sam
author_facet Adonai, Nora
Nguyen, Khoi N.
Walsh, Joseph
Iyer, M.
Toyokuni, Tatsushi
Phelps, Michael E.
McCarthy, Timothy
McCarthy, Deborah W.
Gambhir, Sanjiv Sam
author_sort Adonai, Nora
title Ex vivo cell labeling with (64)Cu–pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
title_short Ex vivo cell labeling with (64)Cu–pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
title_full Ex vivo cell labeling with (64)Cu–pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
title_fullStr Ex vivo cell labeling with (64)Cu–pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
title_full_unstemmed Ex vivo cell labeling with (64)Cu–pyruvaldehyde-bis(N(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
title_sort ex vivo cell labeling with (64)cu–pyruvaldehyde-bis(n(4)-methylthiosemicarbazone) for imaging cell trafficking in mice with positron-emission tomography
publisher National Academy of Sciences
publisher_facet National Academy of Sciences
publishDate 2002
url https://ncbi.nlm.nih.gov/pmc/articles/PMC122467/
https://ncbi.nlm.nih.gov/pubmed/11867752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052709599
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