Ca(2+) sensor S100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse

This study documents the identity of a calcium- regulated membrane guanylate cyclase transduction system in the photoreceptor-bipolar synaptic region. The guanylate cyclase is the previously characterized ROS-GC1 from the rod outer segments and its modulator is S100β. S100β senses increments in free...

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Autors principals: Duda, Teresa, Koch, Karl-Wilhelm, Venkataraman, Venkateswar, Lange, Christian, Beyermann, Michael, Sharma, Rameshwar K.
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Publicat: Oxford University Press 2002
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC125384/
https://ncbi.nlm.nih.gov/pubmed/12032068
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/21.11.2547
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spelling pubmed-1253842002-09-19 Ca(2+) sensor S100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse Duda, Teresa Koch, Karl-Wilhelm Venkataraman, Venkateswar Lange, Christian Beyermann, Michael Sharma, Rameshwar K. EMBO J Article This study documents the identity of a calcium- regulated membrane guanylate cyclase transduction system in the photoreceptor-bipolar synaptic region. The guanylate cyclase is the previously characterized ROS-GC1 from the rod outer segments and its modulator is S100β. S100β senses increments in free Ca(2+) and stimulates the cyclase. Specificity of photoreceptor guanylate cyclase activation by S100β is validated by the identification of two S100β-regulatory sites. A combination of peptide competition, surface plasmon resonance binding and deletion mutation studies has been used to show that these sites are specific for S100β and not for another regulator of ROS-GC1, guanylate cyclase-activating protein 1. One site comprises amino acids (aa) Gly962–Asn981, the other, aa Ile1030–Gln1041. The former represents the binding site. The latter binds S100β only marginally, yet it is critical for control of maximal cyclase activity. The findings provide evidence for a new cyclic GMP transduction system in synaptic layers and thereby extend existing concepts of how a membrane-bound guanylate cyclase is regulated by small Ca(2+)-sensor proteins. Oxford University Press 2002-06-03 /pmc/articles/PMC125384/ /pubmed/12032068 http://dx.doi.org/10.1093/emboj/21.11.2547 Text en Copyright © 2002 European Molecular Biology Organization
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Article
spellingShingle Article
Duda, Teresa
Koch, Karl-Wilhelm
Venkataraman, Venkateswar
Lange, Christian
Beyermann, Michael
Sharma, Rameshwar K.
Ca(2+) sensor S100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse
description This study documents the identity of a calcium- regulated membrane guanylate cyclase transduction system in the photoreceptor-bipolar synaptic region. The guanylate cyclase is the previously characterized ROS-GC1 from the rod outer segments and its modulator is S100β. S100β senses increments in free Ca(2+) and stimulates the cyclase. Specificity of photoreceptor guanylate cyclase activation by S100β is validated by the identification of two S100β-regulatory sites. A combination of peptide competition, surface plasmon resonance binding and deletion mutation studies has been used to show that these sites are specific for S100β and not for another regulator of ROS-GC1, guanylate cyclase-activating protein 1. One site comprises amino acids (aa) Gly962–Asn981, the other, aa Ile1030–Gln1041. The former represents the binding site. The latter binds S100β only marginally, yet it is critical for control of maximal cyclase activity. The findings provide evidence for a new cyclic GMP transduction system in synaptic layers and thereby extend existing concepts of how a membrane-bound guanylate cyclase is regulated by small Ca(2+)-sensor proteins.
author Duda, Teresa
Koch, Karl-Wilhelm
Venkataraman, Venkateswar
Lange, Christian
Beyermann, Michael
Sharma, Rameshwar K.
author_facet Duda, Teresa
Koch, Karl-Wilhelm
Venkataraman, Venkateswar
Lange, Christian
Beyermann, Michael
Sharma, Rameshwar K.
author_sort Duda, Teresa
title Ca(2+) sensor S100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse
title_short Ca(2+) sensor S100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse
title_full Ca(2+) sensor S100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse
title_fullStr Ca(2+) sensor S100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse
title_full_unstemmed Ca(2+) sensor S100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse
title_sort ca(2+) sensor s100β-modulated sites of membrane guanylate cyclase in thephotoreceptor-bipolar synapse
publisher Oxford University Press
publisher_facet Oxford University Press
publishDate 2002
url https://ncbi.nlm.nih.gov/pmc/articles/PMC125384/
https://ncbi.nlm.nih.gov/pubmed/12032068
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/21.11.2547
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