Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum.

Despite the fact that Ca2+ transport into the sarcoplasmic reticulum (SR) of muscle cells is electrogenic, a potential difference is not maintained across the SR membrane. To achieve electroneutrality, compensatory charge movement must occur during Ca2+ uptake. To examine the role of Cl- in this cha...

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Главные авторы: Pollock, N S, Kargacin, M E, Kargacin, G J
Формат: Статья
Язык:English
Опубликовано: 1998
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Research Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC1299847/
https://ncbi.nlm.nih.gov/pubmed/9746517
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spelling pubmed-12998472006-01-17 Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum. Pollock, N S Kargacin, M E Kargacin, G J Biophys J Research Article Despite the fact that Ca2+ transport into the sarcoplasmic reticulum (SR) of muscle cells is electrogenic, a potential difference is not maintained across the SR membrane. To achieve electroneutrality, compensatory charge movement must occur during Ca2+ uptake. To examine the role of Cl- in this charge movement in smooth muscle cells, Ca2+ transport into the SR of saponin-permeabilized smooth muscle cells was measured in the presence of various Cl- channel blockers or when I-, Br-, or SO42- was substituted for Cl-. Calcium uptake was inhibited in a dose-dependent manner by 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and by indanyloxyacetic acid 94 (R(+)-IAA-94), but not by niflumic acid or 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNDS). Smooth muscle SR Ca2+ uptake was also partially inhibited by the substitution of SO42- for Cl-, but not when Cl- was replaced by I- or Br-. Neither NPPB nor R(+)-IAA-94 inhibited Ca2+ uptake into cardiac muscle SR vesicles at concentrations that maximally inhibited uptake in smooth muscle cells. These results indicate that Cl- movement is important for charge compensation in smooth muscle cells and that the Cl- channel or channels involved are different in smooth and cardiac muscle cells. 1998-10 /pmc/articles/PMC1299847/ /pubmed/9746517 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Pollock, N S
Kargacin, M E
Kargacin, G J
Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum.
description Despite the fact that Ca2+ transport into the sarcoplasmic reticulum (SR) of muscle cells is electrogenic, a potential difference is not maintained across the SR membrane. To achieve electroneutrality, compensatory charge movement must occur during Ca2+ uptake. To examine the role of Cl- in this charge movement in smooth muscle cells, Ca2+ transport into the SR of saponin-permeabilized smooth muscle cells was measured in the presence of various Cl- channel blockers or when I-, Br-, or SO42- was substituted for Cl-. Calcium uptake was inhibited in a dose-dependent manner by 5-nitro-2-(3-phenylpropylamino) benzoic acid (NPPB) and by indanyloxyacetic acid 94 (R(+)-IAA-94), but not by niflumic acid or 4,4'-dinitrostilbene-2,2'-disulfonic acid (DNDS). Smooth muscle SR Ca2+ uptake was also partially inhibited by the substitution of SO42- for Cl-, but not when Cl- was replaced by I- or Br-. Neither NPPB nor R(+)-IAA-94 inhibited Ca2+ uptake into cardiac muscle SR vesicles at concentrations that maximally inhibited uptake in smooth muscle cells. These results indicate that Cl- movement is important for charge compensation in smooth muscle cells and that the Cl- channel or channels involved are different in smooth and cardiac muscle cells.
author Pollock, N S
Kargacin, M E
Kargacin, G J
author_facet Pollock, N S
Kargacin, M E
Kargacin, G J
author_sort Pollock, N S
title Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum.
title_short Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum.
title_full Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum.
title_fullStr Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum.
title_full_unstemmed Chloride channel blockers inhibit Ca2+ uptake by the smooth muscle sarcoplasmic reticulum.
title_sort chloride channel blockers inhibit ca2+ uptake by the smooth muscle sarcoplasmic reticulum.
publishDate 1998
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1299847/
https://ncbi.nlm.nih.gov/pubmed/9746517
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