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Partitioning of Individual Flexible Polymers into a Nanoscopic Protein Pore
Polymer dynamics are of fundamental importance in materials science, biotechnology, and medicine. However, very little is known about the kinetics of partitioning of flexible polymer molecules into pores of nanometer dimensions. We employed electrical recording to probe the partitioning of single po...
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Biophysical Society
2003
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pubmed-13032112006-01-17 Partitioning of Individual Flexible Polymers into a Nanoscopic Protein Pore Movileanu, Liviu Cheley, Stephen Bayley, Hagan Biophys J Channels, Receptors, and Transporters Polymer dynamics are of fundamental importance in materials science, biotechnology, and medicine. However, very little is known about the kinetics of partitioning of flexible polymer molecules into pores of nanometer dimensions. We employed electrical recording to probe the partitioning of single poly(ethylene glycol) (PEG) molecules, at concentrations near the dilute regime, into the transmembrane β-barrel of individual protein pores formed from staphylococcal α-hemolysin (αHL). The interactions of the α-hemolysin pore with the PEGs (M(w) 940–6000 Da) fell into two classes: short-duration events (τ ∼ 20 μs), ∼85% of the total, and long-duration events (τ ∼ 100 μs), ∼15% of the total. The association rate constants (k(on)) for both classes of events were strongly dependent on polymer mass, and values of k(on) ranged over two orders of magnitude. By contrast, the dissociation rate constants (k(off)) exhibited a weak dependence on mass, suggesting that the polymer chains are largely compacted before they enter the pore, and do not decompact to a significant extent before they exit. The values of k(on) and k(off) were used to determine partition coefficients (Π) for the PEGs between the bulk aqueous phase and the pore lumen. The low values of Π are in keeping with a negligible interaction between the PEG chains and the interior surface of the pore, which is independent of ionic strength. For the long events, values of Π decrease exponentially with polymer mass, according to the scaling law of Daoud and de Gennes. For PEG molecules larger than ∼5 kDa, Π reached a limiting value suggesting that these PEG chains cannot fit entirely into the β-barrel. Biophysical Society 2003-08 /pmc/articles/PMC1303211/ /pubmed/12885637 Text en Copyright © 2003, Biophysical Society |
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US National Library of Medicine |
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PubMed Central |
| language |
English |
| format |
Article |
| topic |
Channels, Receptors, and Transporters |
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Channels, Receptors, and Transporters Movileanu, Liviu Cheley, Stephen Bayley, Hagan Partitioning of Individual Flexible Polymers into a Nanoscopic Protein Pore |
| description |
Polymer dynamics are of fundamental importance in materials science, biotechnology, and medicine. However, very little is known about the kinetics of partitioning of flexible polymer molecules into pores of nanometer dimensions. We employed electrical recording to probe the partitioning of single poly(ethylene glycol) (PEG) molecules, at concentrations near the dilute regime, into the transmembrane β-barrel of individual protein pores formed from staphylococcal α-hemolysin (αHL). The interactions of the α-hemolysin pore with the PEGs (M(w) 940–6000 Da) fell into two classes: short-duration events (τ ∼ 20 μs), ∼85% of the total, and long-duration events (τ ∼ 100 μs), ∼15% of the total. The association rate constants (k(on)) for both classes of events were strongly dependent on polymer mass, and values of k(on) ranged over two orders of magnitude. By contrast, the dissociation rate constants (k(off)) exhibited a weak dependence on mass, suggesting that the polymer chains are largely compacted before they enter the pore, and do not decompact to a significant extent before they exit. The values of k(on) and k(off) were used to determine partition coefficients (Π) for the PEGs between the bulk aqueous phase and the pore lumen. The low values of Π are in keeping with a negligible interaction between the PEG chains and the interior surface of the pore, which is independent of ionic strength. For the long events, values of Π decrease exponentially with polymer mass, according to the scaling law of Daoud and de Gennes. For PEG molecules larger than ∼5 kDa, Π reached a limiting value suggesting that these PEG chains cannot fit entirely into the β-barrel. |
| author |
Movileanu, Liviu Cheley, Stephen Bayley, Hagan |
| author_facet |
Movileanu, Liviu Cheley, Stephen Bayley, Hagan |
| author_sort |
Movileanu, Liviu |
| title |
Partitioning of Individual Flexible Polymers into a Nanoscopic Protein Pore |
| title_short |
Partitioning of Individual Flexible Polymers into a Nanoscopic Protein Pore |
| title_full |
Partitioning of Individual Flexible Polymers into a Nanoscopic Protein Pore |
| title_fullStr |
Partitioning of Individual Flexible Polymers into a Nanoscopic Protein Pore |
| title_full_unstemmed |
Partitioning of Individual Flexible Polymers into a Nanoscopic Protein Pore |
| title_sort |
partitioning of individual flexible polymers into a nanoscopic protein pore |
| publisher |
Biophysical Society |
| publisher_facet |
Biophysical Society |
| publishDate |
2003 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC1303211/ https://ncbi.nlm.nih.gov/pubmed/12885637 |
| _version_ |
1760292224407437312 |