Endosymbiotic Bacteroidales Bacteria of the Flagellated Protist Pseudotrichonympha grassii in the Gut of the Termite Coptotermes formosanus

A unique lineage of bacteria belonging to the order Bacteroidales was identified as an intracellular endosymbiont of the protist Pseudotrichonympha grassii (Parabasalia, Hypermastigea) in the gut of the termite Coptotermes formosanus. We identified the 16S rRNA, gyrB, elongation factor Tu, and groEL...

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Main Authors: Noda, Satoko, Iida, Toshiya, Kitade, Osamu, Nakajima, Hideaki, Kudo, Toshiaki, Ohkuma, Moriya
Formato: Artigo
Idioma:English
Publicado em: American Society for Microbiology 2005
Assuntos:
Invertebrate Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1317455/
https://ncbi.nlm.nih.gov/pubmed/16332877
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8811-8817.2005
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spelling pubmed-13174552006-02-23 Endosymbiotic Bacteroidales Bacteria of the Flagellated Protist Pseudotrichonympha grassii in the Gut of the Termite Coptotermes formosanus Noda, Satoko Iida, Toshiya Kitade, Osamu Nakajima, Hideaki Kudo, Toshiaki Ohkuma, Moriya Appl Environ Microbiol Invertebrate Microbiology A unique lineage of bacteria belonging to the order Bacteroidales was identified as an intracellular endosymbiont of the protist Pseudotrichonympha grassii (Parabasalia, Hypermastigea) in the gut of the termite Coptotermes formosanus. We identified the 16S rRNA, gyrB, elongation factor Tu, and groEL gene sequences in the endosymbiont and detected a very low level of sequence divergence (<0.9% of the nucleotides) in the endosymbiont population within and among protist cells. The Bacteroidales endosymbiont sequence was affiliated with a cluster comprising only sequences from termite gut bacteria and was not closely related to sequences identified for members of the Bacteroidales attached to the cell surfaces of other gut protists. Transmission electron microscopy showed that there were numerous rod-shaped bacteria in the cytoplasm of the host protist, and we detected the endosymbiont by fluorescence in situ hybridization (FISH) with an oligonucleotide probe specific for the 16S rRNA gene identified. Quantification of the abundance of the Bacteroidales endosymbiont by sequence-specific cleavage of rRNA with RNase H and FISH cell counting revealed, surprisingly, that the endosymbiont accounted for 82% of the total bacterial rRNA and 71% of the total bacterial cells in the gut community. The genetically nearly homogeneous endosymbionts of Pseudotrichonympha were very abundant in the gut symbiotic community of the termite. American Society for Microbiology 2005-12 /pmc/articles/PMC1317455/ /pubmed/16332877 http://dx.doi.org/10.1128/AEM.71.12.8811-8817.2005 Text en Copyright © 2005, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Invertebrate Microbiology
spellingShingle Invertebrate Microbiology
Noda, Satoko
Iida, Toshiya
Kitade, Osamu
Nakajima, Hideaki
Kudo, Toshiaki
Ohkuma, Moriya
Endosymbiotic Bacteroidales Bacteria of the Flagellated Protist Pseudotrichonympha grassii in the Gut of the Termite Coptotermes formosanus
description A unique lineage of bacteria belonging to the order Bacteroidales was identified as an intracellular endosymbiont of the protist Pseudotrichonympha grassii (Parabasalia, Hypermastigea) in the gut of the termite Coptotermes formosanus. We identified the 16S rRNA, gyrB, elongation factor Tu, and groEL gene sequences in the endosymbiont and detected a very low level of sequence divergence (<0.9% of the nucleotides) in the endosymbiont population within and among protist cells. The Bacteroidales endosymbiont sequence was affiliated with a cluster comprising only sequences from termite gut bacteria and was not closely related to sequences identified for members of the Bacteroidales attached to the cell surfaces of other gut protists. Transmission electron microscopy showed that there were numerous rod-shaped bacteria in the cytoplasm of the host protist, and we detected the endosymbiont by fluorescence in situ hybridization (FISH) with an oligonucleotide probe specific for the 16S rRNA gene identified. Quantification of the abundance of the Bacteroidales endosymbiont by sequence-specific cleavage of rRNA with RNase H and FISH cell counting revealed, surprisingly, that the endosymbiont accounted for 82% of the total bacterial rRNA and 71% of the total bacterial cells in the gut community. The genetically nearly homogeneous endosymbionts of Pseudotrichonympha were very abundant in the gut symbiotic community of the termite.
author Noda, Satoko
Iida, Toshiya
Kitade, Osamu
Nakajima, Hideaki
Kudo, Toshiaki
Ohkuma, Moriya
author_facet Noda, Satoko
Iida, Toshiya
Kitade, Osamu
Nakajima, Hideaki
Kudo, Toshiaki
Ohkuma, Moriya
author_sort Noda, Satoko
title Endosymbiotic Bacteroidales Bacteria of the Flagellated Protist Pseudotrichonympha grassii in the Gut of the Termite Coptotermes formosanus
title_short Endosymbiotic Bacteroidales Bacteria of the Flagellated Protist Pseudotrichonympha grassii in the Gut of the Termite Coptotermes formosanus
title_full Endosymbiotic Bacteroidales Bacteria of the Flagellated Protist Pseudotrichonympha grassii in the Gut of the Termite Coptotermes formosanus
title_fullStr Endosymbiotic Bacteroidales Bacteria of the Flagellated Protist Pseudotrichonympha grassii in the Gut of the Termite Coptotermes formosanus
title_full_unstemmed Endosymbiotic Bacteroidales Bacteria of the Flagellated Protist Pseudotrichonympha grassii in the Gut of the Termite Coptotermes formosanus
title_sort endosymbiotic bacteroidales bacteria of the flagellated protist pseudotrichonympha grassii in the gut of the termite coptotermes formosanus
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2005
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1317455/
https://ncbi.nlm.nih.gov/pubmed/16332877
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.71.12.8811-8817.2005
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