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Transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: Implications for Pierson syndrome
Pierson syndrome is a recently defined disease usually lethal within the first postnatal months and caused by mutations in the gene encoding laminin β2 (LAMB2). The hallmarks of Pierson syndrome are congenital nephrotic syndrome accompanied by ocular abnormalities, including microcoria (small pupils...
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2006
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| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1363729/ https://ncbi.nlm.nih.gov/pubmed/16452099 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.02270 |
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pubmed-13637292006-09-01 Transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: Implications for Pierson syndrome Miner, Jeffrey H. Go, Gloriosa Cunningham, Jeanette Patton, Bruce L. Jarad, George Development Article Pierson syndrome is a recently defined disease usually lethal within the first postnatal months and caused by mutations in the gene encoding laminin β2 (LAMB2). The hallmarks of Pierson syndrome are congenital nephrotic syndrome accompanied by ocular abnormalities, including microcoria (small pupils), with muscular and neurological developmental defects also present. Lamb2−/− mice are a model for Pierson syndrome; they exhibit defects in the kidney glomerular barrier, in the development and organization of the neuromuscular junction, and in the retina. Lamb2−/− mice fail to thrive and die very small at 3 weeks of age, but to what extent the kidney and neuromuscular defects each contribute to this severe phenotype has been obscure, though highly relevant to understanding Pierson syndrome. To investigate this, we generated transgenic mouse lines expressing rat laminin β2 either in muscle or in glomerular epithelial cells (podocytes) and crossed them onto the Lamb2−/− background. Rat β2 was confined in skeletal muscle to synapses and myotendinous junctions, and in kidney to the glomerular basement membrane. In transgenic Lamb2−/− mice, β2 deposition only in glomeruli prevented proteinuria but did not ameliorate the severe phenotype. In contrast, β2 expression only in muscle restored synaptic architecture and led to greatly improved health, but the mice died from kidney disease at 1 month. Rescue of both glomeruli and synapses was associated with normal weight gain, fertility, and lifespan. We conclude that muscle defects in Lamb2−/− mice are responsible for the severe failure to thrive phenotype, and that renal replacement therapy alone will be an inadequate treatment for Pierson syndrome. 2006-02-01 2006-03 /pmc/articles/PMC1363729/ /pubmed/16452099 http://dx.doi.org/10.1242/dev.02270 Text en |
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US National Library of Medicine |
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PubMed Central |
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English |
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Article |
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Article |
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Article Miner, Jeffrey H. Go, Gloriosa Cunningham, Jeanette Patton, Bruce L. Jarad, George Transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: Implications for Pierson syndrome |
| description |
Pierson syndrome is a recently defined disease usually lethal within the first postnatal months and caused by mutations in the gene encoding laminin β2 (LAMB2). The hallmarks of Pierson syndrome are congenital nephrotic syndrome accompanied by ocular abnormalities, including microcoria (small pupils), with muscular and neurological developmental defects also present. Lamb2−/− mice are a model for Pierson syndrome; they exhibit defects in the kidney glomerular barrier, in the development and organization of the neuromuscular junction, and in the retina. Lamb2−/− mice fail to thrive and die very small at 3 weeks of age, but to what extent the kidney and neuromuscular defects each contribute to this severe phenotype has been obscure, though highly relevant to understanding Pierson syndrome. To investigate this, we generated transgenic mouse lines expressing rat laminin β2 either in muscle or in glomerular epithelial cells (podocytes) and crossed them onto the Lamb2−/− background. Rat β2 was confined in skeletal muscle to synapses and myotendinous junctions, and in kidney to the glomerular basement membrane. In transgenic Lamb2−/− mice, β2 deposition only in glomeruli prevented proteinuria but did not ameliorate the severe phenotype. In contrast, β2 expression only in muscle restored synaptic architecture and led to greatly improved health, but the mice died from kidney disease at 1 month. Rescue of both glomeruli and synapses was associated with normal weight gain, fertility, and lifespan. We conclude that muscle defects in Lamb2−/− mice are responsible for the severe failure to thrive phenotype, and that renal replacement therapy alone will be an inadequate treatment for Pierson syndrome. |
| author |
Miner, Jeffrey H. Go, Gloriosa Cunningham, Jeanette Patton, Bruce L. Jarad, George |
| author_facet |
Miner, Jeffrey H. Go, Gloriosa Cunningham, Jeanette Patton, Bruce L. Jarad, George |
| author_sort |
Miner, Jeffrey H. |
| title |
Transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: Implications for Pierson syndrome |
| title_short |
Transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: Implications for Pierson syndrome |
| title_full |
Transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: Implications for Pierson syndrome |
| title_fullStr |
Transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: Implications for Pierson syndrome |
| title_full_unstemmed |
Transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: Implications for Pierson syndrome |
| title_sort |
transgenic isolation of skeletal muscle and kidney defects in laminin beta2 mutant mice: implications for pierson syndrome |
| publishDate |
2006 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC1363729/ https://ncbi.nlm.nih.gov/pubmed/16452099 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.02270 |
| _version_ |
1760299738167508992 |