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Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae
Weak organic acids are used as food preservatives to inhibit the growth of spoilage yeasts, including Saccharomyces cerevisiae. Long-term adaptation to weak acids requires the increased expression of the ATP-binding cassette transporter Pdr12p, which catalyses the active efflux of the weak acids fro...
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| Kieli: | English |
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Portland Press Ltd.
2006
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| Linkit: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1409697/ https://ncbi.nlm.nih.gov/pubmed/16316315 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20051765 |
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pubmed-14096972006-10-01 Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae Mollapour, Mehdi Phelan, John P. Millson, Stefan H. Piper, Peter W. Cooke, Frank T. Biochem J Research Article Weak organic acids are used as food preservatives to inhibit the growth of spoilage yeasts, including Saccharomyces cerevisiae. Long-term adaptation to weak acids requires the increased expression of the ATP-binding cassette transporter Pdr12p, which catalyses the active efflux of the weak acids from the cytosol; however, very little is known about the signalling events immediately following application of weak acid stress. We have investigated the effects of weak acids on two stress-responsive signalling molecules, PtdIns(3,5)P(2) and PtdIns(4,5)P(2), which in S. cerevisiae are synthesized by Fab1p and Mss4p respectively. At low extracellular pH, benzoic acid, sorbic acid and acetic acid all cause a transient reduction in PtdIns(3,5)P(2) accumulation and a more persistent rise in PtdIns(4,5)P(2) levels. The increase in PtdIns(4,5)P(2) levels is accompanied by a reorganization of the actin cytoskeleton. However, changes in PtdInsP(2) levels are independent of weak acid-induced Pdr12p expression. In contrast, changing the extracellular medium to alkaline pH provokes a prolonged and substantial rise in PtdIns(3,5)P(2) levels. As PtdIns(3,5)P(2) synthesis is required for correct vacuole acidification, it is possible that levels of this molecule are modulated to maintain intracellular pH homoeostasis in response to weak acid and alkali stresses. In conclusion, we have expanded the repertoire of stress responses that affect PtdInsP(2) levels to include weak acid and alkali stresses. Portland Press Ltd. 2006-03-15 2006-04-01 /pmc/articles/PMC1409697/ /pubmed/16316315 http://dx.doi.org/10.1042/BJ20051765 Text en The Biochemical Society, London |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
English |
| format |
Article |
| topic |
Research Article |
| spellingShingle |
Research Article Mollapour, Mehdi Phelan, John P. Millson, Stefan H. Piper, Peter W. Cooke, Frank T. Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae |
| description |
Weak organic acids are used as food preservatives to inhibit the growth of spoilage yeasts, including Saccharomyces cerevisiae. Long-term adaptation to weak acids requires the increased expression of the ATP-binding cassette transporter Pdr12p, which catalyses the active efflux of the weak acids from the cytosol; however, very little is known about the signalling events immediately following application of weak acid stress. We have investigated the effects of weak acids on two stress-responsive signalling molecules, PtdIns(3,5)P(2) and PtdIns(4,5)P(2), which in S. cerevisiae are synthesized by Fab1p and Mss4p respectively. At low extracellular pH, benzoic acid, sorbic acid and acetic acid all cause a transient reduction in PtdIns(3,5)P(2) accumulation and a more persistent rise in PtdIns(4,5)P(2) levels. The increase in PtdIns(4,5)P(2) levels is accompanied by a reorganization of the actin cytoskeleton. However, changes in PtdInsP(2) levels are independent of weak acid-induced Pdr12p expression. In contrast, changing the extracellular medium to alkaline pH provokes a prolonged and substantial rise in PtdIns(3,5)P(2) levels. As PtdIns(3,5)P(2) synthesis is required for correct vacuole acidification, it is possible that levels of this molecule are modulated to maintain intracellular pH homoeostasis in response to weak acid and alkali stresses. In conclusion, we have expanded the repertoire of stress responses that affect PtdInsP(2) levels to include weak acid and alkali stresses. |
| author |
Mollapour, Mehdi Phelan, John P. Millson, Stefan H. Piper, Peter W. Cooke, Frank T. |
| author_facet |
Mollapour, Mehdi Phelan, John P. Millson, Stefan H. Piper, Peter W. Cooke, Frank T. |
| author_sort |
Mollapour, Mehdi |
| title |
Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae |
| title_short |
Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae |
| title_full |
Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae |
| title_fullStr |
Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae |
| title_full_unstemmed |
Weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in Saccharomyces cerevisiae |
| title_sort |
weak acid and alkali stress regulate phosphatidylinositol bisphosphate synthesis in saccharomyces cerevisiae |
| publisher |
Portland Press Ltd. |
| publisher_facet |
Portland Press Ltd. |
| publishDate |
2006 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC1409697/ https://ncbi.nlm.nih.gov/pubmed/16316315 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1042/BJ20051765 |
| _version_ |
1760305260512935936 |