An analysis of transvection at the yellow locus of Drosophila melanogaster.

Studies of a wide variety of organisms have shown that homologous sequences can exert a significant impact on each other, resulting in changes in gene sequence, gene expression, chromatin structure, and global chromosome architecture. Our work has focused on transvection, a process that can cause ge...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Morris, J R, Chen, J, Filandrinos, S T, Dunn, R C, Fisk, R, Geyer, P K, Wu, C
Formato: Artículo
Lenguaje:English
Publicado: 1999
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1460495/
https://ncbi.nlm.nih.gov/pubmed/9927457
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id pubmed-1460495
record_format dspace
spelling pubmed-14604952006-09-13 An analysis of transvection at the yellow locus of Drosophila melanogaster. Morris, J R Chen, J Filandrinos, S T Dunn, R C Fisk, R Geyer, P K Wu, C Genetics Research Article Studies of a wide variety of organisms have shown that homologous sequences can exert a significant impact on each other, resulting in changes in gene sequence, gene expression, chromatin structure, and global chromosome architecture. Our work has focused on transvection, a process that can cause genes to be sensitive to the proximity of a homologue. Transvection is seen at the yellow gene of Drosophila, where it mediates numerous cases of intragenic complementation. In this article, we describe two approaches that have characterized the process of transvection at yellow. The first entailed a screen for mutations that support intragenic complementation at yellow. The second involved the analysis of 53 yellow alleles, obtained from a variety of sources, with respect to complementation, molecular structure, and transcriptional competence. Our data suggest two ways in which transvection may be regulated at yellow: (1) a transcriptional mechanism, whereby the ability of an allele to support transvection is influenced by its transcriptional competency, and (2) a structural mechanism, whereby the pairing of structurally dissimilar homologues results in conformational changes that affect gene expression. 1999-02 /pmc/articles/PMC1460495/ /pubmed/9927457 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Morris, J R
Chen, J
Filandrinos, S T
Dunn, R C
Fisk, R
Geyer, P K
Wu, C
An analysis of transvection at the yellow locus of Drosophila melanogaster.
description Studies of a wide variety of organisms have shown that homologous sequences can exert a significant impact on each other, resulting in changes in gene sequence, gene expression, chromatin structure, and global chromosome architecture. Our work has focused on transvection, a process that can cause genes to be sensitive to the proximity of a homologue. Transvection is seen at the yellow gene of Drosophila, where it mediates numerous cases of intragenic complementation. In this article, we describe two approaches that have characterized the process of transvection at yellow. The first entailed a screen for mutations that support intragenic complementation at yellow. The second involved the analysis of 53 yellow alleles, obtained from a variety of sources, with respect to complementation, molecular structure, and transcriptional competence. Our data suggest two ways in which transvection may be regulated at yellow: (1) a transcriptional mechanism, whereby the ability of an allele to support transvection is influenced by its transcriptional competency, and (2) a structural mechanism, whereby the pairing of structurally dissimilar homologues results in conformational changes that affect gene expression.
author Morris, J R
Chen, J
Filandrinos, S T
Dunn, R C
Fisk, R
Geyer, P K
Wu, C
author_facet Morris, J R
Chen, J
Filandrinos, S T
Dunn, R C
Fisk, R
Geyer, P K
Wu, C
author_sort Morris, J R
title An analysis of transvection at the yellow locus of Drosophila melanogaster.
title_short An analysis of transvection at the yellow locus of Drosophila melanogaster.
title_full An analysis of transvection at the yellow locus of Drosophila melanogaster.
title_fullStr An analysis of transvection at the yellow locus of Drosophila melanogaster.
title_full_unstemmed An analysis of transvection at the yellow locus of Drosophila melanogaster.
title_sort analysis of transvection at the yellow locus of drosophila melanogaster.
publishDate 1999
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1460495/
https://ncbi.nlm.nih.gov/pubmed/9927457
_version_ 1760311650580168704