Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response

Chlamydia pneumoniae is an obligate intracellular pathogen that causes both acute and chronic human disease. Several in vitro models of chlamydial persistence have been established to mimic chlamydial persistence in vivo. We determined the expression patterns of 52 C. pneumoniae proteins, representi...

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Glavni autori: Mukhopadhyay, Sanghamitra, Miller, Richard D., Sullivan, Erin D., Theodoropoulos, Christina, Mathews, Sarah A., Timms, Peter, Summersgill, James T.
Format: Članak
Jezik:English
Izdano: American Society for Microbiology 2006
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Molecular Pathogenesis
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1489704/
https://ncbi.nlm.nih.gov/pubmed/16790757
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.02104-05
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spelling pubmed-14897042006-11-01 Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response Mukhopadhyay, Sanghamitra Miller, Richard D. Sullivan, Erin D. Theodoropoulos, Christina Mathews, Sarah A. Timms, Peter Summersgill, James T. Infect Immun Molecular Pathogenesis Chlamydia pneumoniae is an obligate intracellular pathogen that causes both acute and chronic human disease. Several in vitro models of chlamydial persistence have been established to mimic chlamydial persistence in vivo. We determined the expression patterns of 52 C. pneumoniae proteins, representing nine functional subgroups, from the gamma interferon (IFN-γ) treatment (primarily tryptophan limitation) and iron limitation (IL) models of persistence compared to those following heat shock (HS) at 42°C. Protein expression patterns of C. pneumoniae persistence indicates a strong stress component, as evidenced by the upregulation of proteins involved in protein folding, assembly, and modification. However, it is clearly more than just a stress response. In IFN persistence, but not IL or HS, amino acid and/or nucleotide biosynthesis proteins were found to be significantly upregulated. In contrast, proteins involved in the biosynthesis of cofactors, cellular processes, energy metabolism, transcription, and translation showed an increased in expression in only the IL model of persistence. These data represent the most extensive protein expression study of C. pneumoniae comparing the chlamydial heat shock stress response to two models of persistence and identifying the common and unique protein level responses during persistence. American Society for Microbiology 2006-07 /pmc/articles/PMC1489704/ /pubmed/16790757 http://dx.doi.org/10.1128/IAI.02104-05 Text en Copyright © 2006, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Molecular Pathogenesis
spellingShingle Molecular Pathogenesis
Mukhopadhyay, Sanghamitra
Miller, Richard D.
Sullivan, Erin D.
Theodoropoulos, Christina
Mathews, Sarah A.
Timms, Peter
Summersgill, James T.
Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response
description Chlamydia pneumoniae is an obligate intracellular pathogen that causes both acute and chronic human disease. Several in vitro models of chlamydial persistence have been established to mimic chlamydial persistence in vivo. We determined the expression patterns of 52 C. pneumoniae proteins, representing nine functional subgroups, from the gamma interferon (IFN-γ) treatment (primarily tryptophan limitation) and iron limitation (IL) models of persistence compared to those following heat shock (HS) at 42°C. Protein expression patterns of C. pneumoniae persistence indicates a strong stress component, as evidenced by the upregulation of proteins involved in protein folding, assembly, and modification. However, it is clearly more than just a stress response. In IFN persistence, but not IL or HS, amino acid and/or nucleotide biosynthesis proteins were found to be significantly upregulated. In contrast, proteins involved in the biosynthesis of cofactors, cellular processes, energy metabolism, transcription, and translation showed an increased in expression in only the IL model of persistence. These data represent the most extensive protein expression study of C. pneumoniae comparing the chlamydial heat shock stress response to two models of persistence and identifying the common and unique protein level responses during persistence.
author Mukhopadhyay, Sanghamitra
Miller, Richard D.
Sullivan, Erin D.
Theodoropoulos, Christina
Mathews, Sarah A.
Timms, Peter
Summersgill, James T.
author_facet Mukhopadhyay, Sanghamitra
Miller, Richard D.
Sullivan, Erin D.
Theodoropoulos, Christina
Mathews, Sarah A.
Timms, Peter
Summersgill, James T.
author_sort Mukhopadhyay, Sanghamitra
title Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response
title_short Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response
title_full Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response
title_fullStr Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response
title_full_unstemmed Protein Expression Profiles of Chlamydia pneumoniae in Models of Persistence versus Those of Heat Shock Stress Response
title_sort protein expression profiles of chlamydia pneumoniae in models of persistence versus those of heat shock stress response
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2006
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1489704/
https://ncbi.nlm.nih.gov/pubmed/16790757
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.02104-05
_version_ 1760315217775951872

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