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Ergosterol is required for the Sec18/ATP-dependent priming step of homotypic vacuole fusion
In vitro homotypic fusion of yeast vacuoles occurs in three stages: priming, the Sec18 (NSF)-mediated changes that precede vacuole association; docking, the Ypt7 and SNARE-mediated pairing of vacuoles; and fusion, mediated by calmodulin/V0/t-SNARE interactions. Defects in catalysts of each stage res...
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| Главные авторы: | , |
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| Формат: | Статья |
| Язык: | en |
| Опубликовано: |
Oxford University Press
2001
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC149151/ https://ncbi.nlm.nih.gov/pubmed/11483507 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.15.4035 |
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