Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo

Ovine neutrophils spontaneously underwent apoptosis during culture in vitro, as assessed by morphological changes and exposure of annexin V binding sites on their cell surfaces. The addition of conditioned medium from concanavalin A-treated ovine peripheral blood mononuclear cells (PBMC) could parti...

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Main Authors: Scaife, Helena, Woldehiwet, Zerai, Hart, C. Anthony, Edwards, Steven W.
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology (ASM) 2003
Assuntos:
Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC152089/
https://ncbi.nlm.nih.gov/pubmed/12654818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.71.4.1995-2001.2003
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spelling pubmed-1520892003-05-19 Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo Scaife, Helena Woldehiwet, Zerai Hart, C. Anthony Edwards, Steven W. Infect Immun Cellular Microbiology: Pathogen-Host Cell Molecular Interactions Ovine neutrophils spontaneously underwent apoptosis during culture in vitro, as assessed by morphological changes and exposure of annexin V binding sites on their cell surfaces. The addition of conditioned medium from concanavalin A-treated ovine peripheral blood mononuclear cells (PBMC) could partially protect against this progression into apoptosis, but dexamethasone and sodium butyrate could not. Actinomycin D accelerated the rate at which ovine neutrophils underwent apoptosis. Neutrophils isolated from sheep experimentally infected with Anaplasma phagocytophilum showed significantly delayed apoptosis during culture ex vivo, and the addition of conditioned medium from PBMC to these cells could not delay apoptosis above the protective effects observed after in vivo infection. The ability of neutrophils from A. phagocytophilum-infected sheep to activate a respiratory burst was increased compared to the activity measured in neutrophils from uninfected sheep, but chemotaxis was decreased in neutrophils from infected sheep. These data are the first demonstration that in vivo infection with A. phagocytophilum results in changes in rates of apoptosis of infected immune cells. This may help explain how these bacteria replicate in these normally short-lived cells. American Society for Microbiology (ASM) 2003-04 /pmc/articles/PMC152089/ /pubmed/12654818 http://dx.doi.org/10.1128/IAI.71.4.1995-2001.2003 Text en Copyright © 2003, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
spellingShingle Cellular Microbiology: Pathogen-Host Cell Molecular Interactions
Scaife, Helena
Woldehiwet, Zerai
Hart, C. Anthony
Edwards, Steven W.
Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo
description Ovine neutrophils spontaneously underwent apoptosis during culture in vitro, as assessed by morphological changes and exposure of annexin V binding sites on their cell surfaces. The addition of conditioned medium from concanavalin A-treated ovine peripheral blood mononuclear cells (PBMC) could partially protect against this progression into apoptosis, but dexamethasone and sodium butyrate could not. Actinomycin D accelerated the rate at which ovine neutrophils underwent apoptosis. Neutrophils isolated from sheep experimentally infected with Anaplasma phagocytophilum showed significantly delayed apoptosis during culture ex vivo, and the addition of conditioned medium from PBMC to these cells could not delay apoptosis above the protective effects observed after in vivo infection. The ability of neutrophils from A. phagocytophilum-infected sheep to activate a respiratory burst was increased compared to the activity measured in neutrophils from uninfected sheep, but chemotaxis was decreased in neutrophils from infected sheep. These data are the first demonstration that in vivo infection with A. phagocytophilum results in changes in rates of apoptosis of infected immune cells. This may help explain how these bacteria replicate in these normally short-lived cells.
author Scaife, Helena
Woldehiwet, Zerai
Hart, C. Anthony
Edwards, Steven W.
author_facet Scaife, Helena
Woldehiwet, Zerai
Hart, C. Anthony
Edwards, Steven W.
author_sort Scaife, Helena
title Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo
title_short Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo
title_full Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo
title_fullStr Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo
title_full_unstemmed Anaplasma phagocytophilum Reduces Neutrophil Apoptosis In Vivo
title_sort anaplasma phagocytophilum reduces neutrophil apoptosis in vivo
publisher American Society for Microbiology (ASM)
publisher_facet American Society for Microbiology (ASM)
publishDate 2003
url https://ncbi.nlm.nih.gov/pmc/articles/PMC152089/
https://ncbi.nlm.nih.gov/pubmed/12654818
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/IAI.71.4.1995-2001.2003
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