Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein

The cellular HERV-W envelope/syncytin-1 protein, encoded by the envelope gene of the ERVWE1 proviral locus is a fusogenic glycoprotein probably involved in the formation of the placental syncytiotrophoblast layer. Syncytin-1-induced in vitro cell-cell fusion is dependent on the interaction with hASC...

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التفاصيل البيبلوغرافية
المؤلفون الرئيسيون: Cheynet, Valérie, Oriol, Guy, Mallet, François
التنسيق: مقال
اللغة:English
منشور في: BioMed Central 2006
الموضوعات:
Short Report
الوصول للمادة أونلاين:https://ncbi.nlm.nih.gov/pmc/articles/PMC1524976/
https://ncbi.nlm.nih.gov/pubmed/16820059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1742-4690-3-41
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id pubmed-1524976
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spelling pubmed-15249762006-08-01 Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein Cheynet, Valérie Oriol, Guy Mallet, François Retrovirology Short Report The cellular HERV-W envelope/syncytin-1 protein, encoded by the envelope gene of the ERVWE1 proviral locus is a fusogenic glycoprotein probably involved in the formation of the placental syncytiotrophoblast layer. Syncytin-1-induced in vitro cell-cell fusion is dependent on the interaction with hASCT2. As no receptor binding domain has been clearly defined in the SU of neither the HERV-W Env nor the retroviruses of the same interference group, we designed an in vitro binding assay to evaluate the interaction of the HERV-W envelope with the hASCT2 receptor. Using truncated HERV-W SU subunits, a region consisting of the N-terminal 124 amino acids of the mature SU glycoprotein was determined as the minimal receptor-binding domain. This domain contains several sub-domains which are poorly conserved among retroviruses of this interference group but a region of 18 residus containing the SDGGGX(2)DX(2)R conserved motif was proved to be essential for syncytin-1-hASCT2 interaction. BioMed Central 2006-07-04 /pmc/articles/PMC1524976/ /pubmed/16820059 http://dx.doi.org/10.1186/1742-4690-3-41 Text en Copyright © 2006 Cheynet et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Short Report
spellingShingle Short Report
Cheynet, Valérie
Oriol, Guy
Mallet, François
Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein
description The cellular HERV-W envelope/syncytin-1 protein, encoded by the envelope gene of the ERVWE1 proviral locus is a fusogenic glycoprotein probably involved in the formation of the placental syncytiotrophoblast layer. Syncytin-1-induced in vitro cell-cell fusion is dependent on the interaction with hASCT2. As no receptor binding domain has been clearly defined in the SU of neither the HERV-W Env nor the retroviruses of the same interference group, we designed an in vitro binding assay to evaluate the interaction of the HERV-W envelope with the hASCT2 receptor. Using truncated HERV-W SU subunits, a region consisting of the N-terminal 124 amino acids of the mature SU glycoprotein was determined as the minimal receptor-binding domain. This domain contains several sub-domains which are poorly conserved among retroviruses of this interference group but a region of 18 residus containing the SDGGGX(2)DX(2)R conserved motif was proved to be essential for syncytin-1-hASCT2 interaction.
author Cheynet, Valérie
Oriol, Guy
Mallet, François
author_facet Cheynet, Valérie
Oriol, Guy
Mallet, François
author_sort Cheynet, Valérie
title Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein
title_short Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein
title_full Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein
title_fullStr Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein
title_full_unstemmed Identification of the hASCT2-binding domain of the Env ERVWE1/syncytin-1 fusogenic glycoprotein
title_sort identification of the hasct2-binding domain of the env ervwe1/syncytin-1 fusogenic glycoprotein
publisher BioMed Central
publisher_facet BioMed Central
publishDate 2006
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1524976/
https://ncbi.nlm.nih.gov/pubmed/16820059
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1742-4690-3-41
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