Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses

The INFLU A·B-Quick and Directigen Flu A+B enzyme immunoassays were compared with direct immunofluorescence and cell culture for detection of influenza A and B viruses in a total of 255 patient specimens. Both assays identified 23 of 42 influenza A viruses (sensitivity, 54.8%; specificity, 100%; pos...

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Main Authors: Dunn, James J., Gordon, Chris, Kelley, Christy, Carroll, Karen C.
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2003
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC154708/
https://ncbi.nlm.nih.gov/pubmed/12734274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.5.2180-2183.2003
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spelling pubmed-1547082003-06-15 Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses Dunn, James J. Gordon, Chris Kelley, Christy Carroll, Karen C. J Clin Microbiol Virology The INFLU A·B-Quick and Directigen Flu A+B enzyme immunoassays were compared with direct immunofluorescence and cell culture for detection of influenza A and B viruses in a total of 255 patient specimens. Both assays identified 23 of 42 influenza A viruses (sensitivity, 54.8%; specificity, 100%; positive predictive value [PPV], 100%; negative predictive value [NPV], 91.8%). The INFLU A·B-Quick assay identified 10 of 16 influenza B viruses (sensitivity, 62.5%; specificity, 99.6%; PPV, 90.9%; NPV, 97.5%), and the Directigen Flu A+B assay detected 9 of 16 influenza B viruses (sensitivity, 56.3%; specificity, 99.6%; PPV, 90%; NPV, 97.1%). American Society for Microbiology 2003-05 /pmc/articles/PMC154708/ /pubmed/12734274 http://dx.doi.org/10.1128/JCM.41.5.2180-2183.2003 Text en Copyright © 2003, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Virology
spellingShingle Virology
Dunn, James J.
Gordon, Chris
Kelley, Christy
Carroll, Karen C.
Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses
description The INFLU A·B-Quick and Directigen Flu A+B enzyme immunoassays were compared with direct immunofluorescence and cell culture for detection of influenza A and B viruses in a total of 255 patient specimens. Both assays identified 23 of 42 influenza A viruses (sensitivity, 54.8%; specificity, 100%; positive predictive value [PPV], 100%; negative predictive value [NPV], 91.8%). The INFLU A·B-Quick assay identified 10 of 16 influenza B viruses (sensitivity, 62.5%; specificity, 99.6%; PPV, 90.9%; NPV, 97.5%), and the Directigen Flu A+B assay detected 9 of 16 influenza B viruses (sensitivity, 56.3%; specificity, 99.6%; PPV, 90%; NPV, 97.1%).
author Dunn, James J.
Gordon, Chris
Kelley, Christy
Carroll, Karen C.
author_facet Dunn, James J.
Gordon, Chris
Kelley, Christy
Carroll, Karen C.
author_sort Dunn, James J.
title Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses
title_short Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses
title_full Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses
title_fullStr Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses
title_full_unstemmed Comparison of the Denka-Seiken INFLU A·B-Quick and BD Directigen Flu A+B Kits with Direct Fluorescent-Antibody Staining and Shell Vial Culture Methods for Rapid Detection of Influenza Viruses
title_sort comparison of the denka-seiken influ a·b-quick and bd directigen flu a+b kits with direct fluorescent-antibody staining and shell vial culture methods for rapid detection of influenza viruses
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2003
url https://ncbi.nlm.nih.gov/pmc/articles/PMC154708/
https://ncbi.nlm.nih.gov/pubmed/12734274
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.5.2180-2183.2003
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