Conjugative Plasmid Transfer in Gram-Positive Bacteria

Conjugative transfer of bacterial plasmids is the most efficient way of horizontal gene spread, and it is therefore considered one of the major reasons for the increase in the number of bacteria exhibiting multiple-antibiotic resistance. Thus, conjugation and spread of antibiotic resistance represen...

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Main Authors: Grohmann, Elisabeth, Muth, Günther, Espinosa, Manuel
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2003
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Reviews
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC156469/
https://ncbi.nlm.nih.gov/pubmed/12794193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MMBR.67.2.277-301.2003
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spelling pubmed-1564692003-06-12 Conjugative Plasmid Transfer in Gram-Positive Bacteria Grohmann, Elisabeth Muth, Günther Espinosa, Manuel Microbiol Mol Biol Rev Reviews Conjugative transfer of bacterial plasmids is the most efficient way of horizontal gene spread, and it is therefore considered one of the major reasons for the increase in the number of bacteria exhibiting multiple-antibiotic resistance. Thus, conjugation and spread of antibiotic resistance represents a severe problem in antibiotic treatment, especially of immunosuppressed patients and in intensive care units. While conjugation in gram-negative bacteria has been studied in great detail over the last decades, the transfer mechanisms of antibiotic resistance plasmids in gram-positive bacteria remained obscure. In the last few years, the entire nucleotide sequences of several large conjugative plasmids from gram-positive bacteria have been determined. Sequence analyses and data bank comparisons of their putative transfer (tra) regions have revealed significant similarities to tra regions of plasmids from gram-negative bacteria with regard to the respective DNA relaxases and their targets, the origins of transfer (oriT), and putative nucleoside triphosphatases NTP-ases with homologies to type IV secretion systems. In contrast, a single gene encoding a septal DNA translocator protein is involved in plasmid transfer between micelle-forming streptomycetes. Based on these clues, we propose the existence of two fundamentally different plasmid-mediated conjugative mechanisms in gram-positive microorganisms, namely, the mechanism taking place in unicellular gram-positive bacteria, which is functionally similar to that in gram-negative bacteria, and a second type that occurs in multicellular gram-positive bacteria, which seems to be characterized by double-stranded DNA transfer. American Society for Microbiology 2003-06 /pmc/articles/PMC156469/ /pubmed/12794193 http://dx.doi.org/10.1128/MMBR.67.2.277-301.2003 Text en Copyright © 2003, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Reviews
spellingShingle Reviews
Grohmann, Elisabeth
Muth, Günther
Espinosa, Manuel
Conjugative Plasmid Transfer in Gram-Positive Bacteria
description Conjugative transfer of bacterial plasmids is the most efficient way of horizontal gene spread, and it is therefore considered one of the major reasons for the increase in the number of bacteria exhibiting multiple-antibiotic resistance. Thus, conjugation and spread of antibiotic resistance represents a severe problem in antibiotic treatment, especially of immunosuppressed patients and in intensive care units. While conjugation in gram-negative bacteria has been studied in great detail over the last decades, the transfer mechanisms of antibiotic resistance plasmids in gram-positive bacteria remained obscure. In the last few years, the entire nucleotide sequences of several large conjugative plasmids from gram-positive bacteria have been determined. Sequence analyses and data bank comparisons of their putative transfer (tra) regions have revealed significant similarities to tra regions of plasmids from gram-negative bacteria with regard to the respective DNA relaxases and their targets, the origins of transfer (oriT), and putative nucleoside triphosphatases NTP-ases with homologies to type IV secretion systems. In contrast, a single gene encoding a septal DNA translocator protein is involved in plasmid transfer between micelle-forming streptomycetes. Based on these clues, we propose the existence of two fundamentally different plasmid-mediated conjugative mechanisms in gram-positive microorganisms, namely, the mechanism taking place in unicellular gram-positive bacteria, which is functionally similar to that in gram-negative bacteria, and a second type that occurs in multicellular gram-positive bacteria, which seems to be characterized by double-stranded DNA transfer.
author Grohmann, Elisabeth
Muth, Günther
Espinosa, Manuel
author_facet Grohmann, Elisabeth
Muth, Günther
Espinosa, Manuel
author_sort Grohmann, Elisabeth
title Conjugative Plasmid Transfer in Gram-Positive Bacteria
title_short Conjugative Plasmid Transfer in Gram-Positive Bacteria
title_full Conjugative Plasmid Transfer in Gram-Positive Bacteria
title_fullStr Conjugative Plasmid Transfer in Gram-Positive Bacteria
title_full_unstemmed Conjugative Plasmid Transfer in Gram-Positive Bacteria
title_sort conjugative plasmid transfer in gram-positive bacteria
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2003
url https://ncbi.nlm.nih.gov/pmc/articles/PMC156469/
https://ncbi.nlm.nih.gov/pubmed/12794193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MMBR.67.2.277-301.2003
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