The patterns of gene expression in the tomato shoot apical meristem.

In this paper, we describe the synthesis of a cDNA library from the vegetative shoot apical meristem and the analysis of clones selected from it. Using in situ hybridization, we characterized the patterns of expression of these genes in the tomato shoot apical meristem, as well as the patterns obtai...

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Main Authors: Fleming, A J, Mandel, T, Roth, I, Kuhlemeier, C
Formato: Artigo
Idioma:en
Publicado em: 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC160271/
https://ncbi.nlm.nih.gov/pubmed/8467223
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spelling pubmed-1602712003-07-13 The patterns of gene expression in the tomato shoot apical meristem. Fleming, A J Mandel, T Roth, I Kuhlemeier, C Plant Cell Research Article In this paper, we describe the synthesis of a cDNA library from the vegetative shoot apical meristem and the analysis of clones selected from it. Using in situ hybridization, we characterized the patterns of expression of these genes in the tomato shoot apical meristem, as well as the patterns obtained from other sources. The results from the analysis of 15 cDNAs indicated the following six main patterns of gene expression in the shoot apical meristem: overall expression, zero expression, expression limited to the epidermis, expression excluded from the epidermis, punctate expression, and expression elevated in the flanks of the meristem. The patterns observed and the nature and number of the genes showing these patterns necessitate a reinterpretation of the models of meristem structure and function. In particular, the data suggest a compartmentation within the shoot apical meristem based on the spatial expression of particular subsets of genes. This paper also reports on the specific and precise criteria essential for the correct identification of meristem-specific gene expression. The data give new insight into the molecular organization of the shoot apical meristem and provide the framework for a detailed dissection of the factors controlling this organization. 1993-03 /pmc/articles/PMC160271/ /pubmed/8467223 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Fleming, A J
Mandel, T
Roth, I
Kuhlemeier, C
The patterns of gene expression in the tomato shoot apical meristem.
description In this paper, we describe the synthesis of a cDNA library from the vegetative shoot apical meristem and the analysis of clones selected from it. Using in situ hybridization, we characterized the patterns of expression of these genes in the tomato shoot apical meristem, as well as the patterns obtained from other sources. The results from the analysis of 15 cDNAs indicated the following six main patterns of gene expression in the shoot apical meristem: overall expression, zero expression, expression limited to the epidermis, expression excluded from the epidermis, punctate expression, and expression elevated in the flanks of the meristem. The patterns observed and the nature and number of the genes showing these patterns necessitate a reinterpretation of the models of meristem structure and function. In particular, the data suggest a compartmentation within the shoot apical meristem based on the spatial expression of particular subsets of genes. This paper also reports on the specific and precise criteria essential for the correct identification of meristem-specific gene expression. The data give new insight into the molecular organization of the shoot apical meristem and provide the framework for a detailed dissection of the factors controlling this organization.
author Fleming, A J
Mandel, T
Roth, I
Kuhlemeier, C
author_facet Fleming, A J
Mandel, T
Roth, I
Kuhlemeier, C
author_sort Fleming, A J
title The patterns of gene expression in the tomato shoot apical meristem.
title_short The patterns of gene expression in the tomato shoot apical meristem.
title_full The patterns of gene expression in the tomato shoot apical meristem.
title_fullStr The patterns of gene expression in the tomato shoot apical meristem.
title_full_unstemmed The patterns of gene expression in the tomato shoot apical meristem.
title_sort patterns of gene expression in the tomato shoot apical meristem.
publishDate 1993
url https://ncbi.nlm.nih.gov/pmc/articles/PMC160271/
https://ncbi.nlm.nih.gov/pubmed/8467223
_version_ 1759037399416963072

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