Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans

Telomerase is a ribonucleoprotein enzyme responsible for the addition of telomeres onto the ends of chromosomes. Short or dysfunctional telomeres can lead to cell growth arrest, apoptosis, and genomic instability. Telomerase uses its RNA subunit to copy a short template region for telomere synthesis...

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Main Authors: Mason, Douglas X., Goneska, Elizabeth, Greider, Carol W.
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2003
Assuntos:
DNA Dynamics and Chromosome Structure
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC166324/
https://ncbi.nlm.nih.gov/pubmed/12897134
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.23.16.5606-5613.2003
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spelling pubmed-1663242003-10-21 Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans Mason, Douglas X. Goneska, Elizabeth Greider, Carol W. Mol Cell Biol DNA Dynamics and Chromosome Structure Telomerase is a ribonucleoprotein enzyme responsible for the addition of telomeres onto the ends of chromosomes. Short or dysfunctional telomeres can lead to cell growth arrest, apoptosis, and genomic instability. Telomerase uses its RNA subunit to copy a short template region for telomere synthesis. To probe for regions of Tetrahymena telomerase RNA essential for function, we assayed 27 circularly permuted RNA deletions for telomerase in vitro activity and binding to the telomerase reverse transcriptase catalytic protein subunit. We found that stem-loop IV is required for wild-type telomerase activity in vitro and will stimulate processivity when added in trans. American Society for Microbiology 2003-08 /pmc/articles/PMC166324/ /pubmed/12897134 http://dx.doi.org/10.1128/MCB.23.16.5606-5613.2003 Text en Copyright © 2003, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic DNA Dynamics and Chromosome Structure
spellingShingle DNA Dynamics and Chromosome Structure
Mason, Douglas X.
Goneska, Elizabeth
Greider, Carol W.
Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans
description Telomerase is a ribonucleoprotein enzyme responsible for the addition of telomeres onto the ends of chromosomes. Short or dysfunctional telomeres can lead to cell growth arrest, apoptosis, and genomic instability. Telomerase uses its RNA subunit to copy a short template region for telomere synthesis. To probe for regions of Tetrahymena telomerase RNA essential for function, we assayed 27 circularly permuted RNA deletions for telomerase in vitro activity and binding to the telomerase reverse transcriptase catalytic protein subunit. We found that stem-loop IV is required for wild-type telomerase activity in vitro and will stimulate processivity when added in trans.
author Mason, Douglas X.
Goneska, Elizabeth
Greider, Carol W.
author_facet Mason, Douglas X.
Goneska, Elizabeth
Greider, Carol W.
author_sort Mason, Douglas X.
title Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans
title_short Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans
title_full Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans
title_fullStr Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans
title_full_unstemmed Stem-Loop IV of Tetrahymena Telomerase RNA Stimulates Processivity in trans
title_sort stem-loop iv of tetrahymena telomerase rna stimulates processivity in trans
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2003
url https://ncbi.nlm.nih.gov/pmc/articles/PMC166324/
https://ncbi.nlm.nih.gov/pubmed/12897134
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.23.16.5606-5613.2003
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