Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae

A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-Δ strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcri...

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Main Authors: Das, Biswadip, Butler, J. Scott, Sherman, Fred
Formato: Artigo
Idioma:en
Publicado em: American Society for Microbiology 2003
Assuntos:
Gene Expression
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC166345/
https://ncbi.nlm.nih.gov/pubmed/12897126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.23.16.5502-5515.2003
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spelling pubmed-1663452003-10-21 Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae Das, Biswadip Butler, J. Scott Sherman, Fred Mol Cell Biol Gene Expression A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-Δ strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcription with thiolutin in nup116-Δ strains revealed a rapid degradation of mRNAs in the nucleus that was suppressed by the rrp6-Δ, rai1-Δ, and cbc1-Δ deletions, but not by the upf1-Δ deletion, suggesting that DRN requires Rrp6p, a 3′-to-5′ nuclear exonuclease, the Rat1p, a 5′-to-3′ nuclear exonuclease, and Cbc1p, a component of CBC, the nuclear cap binding complex, which may direct the mRNAs to the site of degradation. We propose that certain normal mRNAs retained in the nucleus are degraded by the DRN system, similar to degradation of transcripts with 3′ end formation defects in certain mutants. American Society for Microbiology 2003-08 /pmc/articles/PMC166345/ /pubmed/12897126 http://dx.doi.org/10.1128/MCB.23.16.5502-5515.2003 Text en Copyright © 2003, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Gene Expression
spellingShingle Gene Expression
Das, Biswadip
Butler, J. Scott
Sherman, Fred
Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae
description A nuclear mRNA degradation (DRN) system was identified from analysis of mRNA turnover rates in nup116-Δ strains of Saccharomyces cerevisiae lacking the ability to export all RNAs, including poly(A) mRNAs, at the restrictive temperature. Northern blotting, in situ hybridization, and blocking transcription with thiolutin in nup116-Δ strains revealed a rapid degradation of mRNAs in the nucleus that was suppressed by the rrp6-Δ, rai1-Δ, and cbc1-Δ deletions, but not by the upf1-Δ deletion, suggesting that DRN requires Rrp6p, a 3′-to-5′ nuclear exonuclease, the Rat1p, a 5′-to-3′ nuclear exonuclease, and Cbc1p, a component of CBC, the nuclear cap binding complex, which may direct the mRNAs to the site of degradation. We propose that certain normal mRNAs retained in the nucleus are degraded by the DRN system, similar to degradation of transcripts with 3′ end formation defects in certain mutants.
author Das, Biswadip
Butler, J. Scott
Sherman, Fred
author_facet Das, Biswadip
Butler, J. Scott
Sherman, Fred
author_sort Das, Biswadip
title Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae
title_short Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae
title_full Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae
title_fullStr Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae
title_full_unstemmed Degradation of Normal mRNA in the Nucleus of Saccharomyces cerevisiae
title_sort degradation of normal mrna in the nucleus of saccharomyces cerevisiae
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2003
url https://ncbi.nlm.nih.gov/pmc/articles/PMC166345/
https://ncbi.nlm.nih.gov/pubmed/12897126
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/MCB.23.16.5502-5515.2003
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