Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family

The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzyme...

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Main Authors: Criqui, Marie Claire, de Almeida Engler, Janice, Camasses, Alain, Capron, Arnaud, Parmentier, Yves, Inzé, Dirk, Genschik, Pascal
Formato: Artigo
Idioma:en
Publicado em: American Society of Plant Biologists 2002
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC166644/
https://ncbi.nlm.nih.gov/pubmed/12427990
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.011353
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spelling pubmed-1666442003-11-01 Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family Criqui, Marie Claire de Almeida Engler, Janice Camasses, Alain Capron, Arnaud Parmentier, Yves Inzé, Dirk Genschik, Pascal Plant Physiol Research Article The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzymes (UBCs) (E2s). One class of E2s (called E2-C) seems specifically involved in cyclin B1 degradation. Although it has recently been shown that mammalian E2-C is regulated at the protein level during the cell cycle, not much is known concerning the expression of these genes. Arabidopsis encodes two genes belonging to the E2-C gene family (called UBC19 and UBC20). We found that UBC19 is able to complement fission yeast (Schizosaccharomyces pombe) UbcP4-140 mutant, indicating that the plant protein can functionally replace its yeast ortholog for protein degradation during mitosis. In situ hybridization experiments were performed to study the expression of the E2-C genes in various tissues of plants. Their transcripts were always, but not exclusively, found in tissues active for cell division. Thus, the UBC19/20 E2s may have a key function during cell cycle, but may also be involved in ubiquitylation reactions occurring during differentiation and/or in differentiated cells. Finally, we showed that a translational fusion protein between UBC19 and green fluorescent protein localized both in the cytosol and the nucleus in stable transformed tobacco (Nicotiana tabacum cv Bright Yellow 2) cells. American Society of Plant Biologists 2002-11 /pmc/articles/PMC166644/ /pubmed/12427990 http://dx.doi.org/10.1104/pp.011353 Text en Copyright © 2002, American Society of Plant Biologists
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Criqui, Marie Claire
de Almeida Engler, Janice
Camasses, Alain
Capron, Arnaud
Parmentier, Yves
Inzé, Dirk
Genschik, Pascal
Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family
description The anaphase promoting complex or cyclosome is the ubiquitin-ligase that targets destruction box-containing proteins for proteolysis during the cell cycle. Anaphase promoting complex or cyclosome and its activator (the fizzy and fizzy-related) proteins work together with ubiquitin-conjugating enzymes (UBCs) (E2s). One class of E2s (called E2-C) seems specifically involved in cyclin B1 degradation. Although it has recently been shown that mammalian E2-C is regulated at the protein level during the cell cycle, not much is known concerning the expression of these genes. Arabidopsis encodes two genes belonging to the E2-C gene family (called UBC19 and UBC20). We found that UBC19 is able to complement fission yeast (Schizosaccharomyces pombe) UbcP4-140 mutant, indicating that the plant protein can functionally replace its yeast ortholog for protein degradation during mitosis. In situ hybridization experiments were performed to study the expression of the E2-C genes in various tissues of plants. Their transcripts were always, but not exclusively, found in tissues active for cell division. Thus, the UBC19/20 E2s may have a key function during cell cycle, but may also be involved in ubiquitylation reactions occurring during differentiation and/or in differentiated cells. Finally, we showed that a translational fusion protein between UBC19 and green fluorescent protein localized both in the cytosol and the nucleus in stable transformed tobacco (Nicotiana tabacum cv Bright Yellow 2) cells.
author Criqui, Marie Claire
de Almeida Engler, Janice
Camasses, Alain
Capron, Arnaud
Parmentier, Yves
Inzé, Dirk
Genschik, Pascal
author_facet Criqui, Marie Claire
de Almeida Engler, Janice
Camasses, Alain
Capron, Arnaud
Parmentier, Yves
Inzé, Dirk
Genschik, Pascal
author_sort Criqui, Marie Claire
title Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family
title_short Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family
title_full Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family
title_fullStr Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family
title_full_unstemmed Molecular Characterization of Plant Ubiquitin-Conjugating Enzymes Belonging to the UbcP4/E2-C/UBCx/UbcH10 Gene Family
title_sort molecular characterization of plant ubiquitin-conjugating enzymes belonging to the ubcp4/e2-c/ubcx/ubch10 gene family
publisher American Society of Plant Biologists
publisher_facet American Society of Plant Biologists
publishDate 2002
url https://ncbi.nlm.nih.gov/pmc/articles/PMC166644/
https://ncbi.nlm.nih.gov/pubmed/12427990
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.011353
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