Differentiation of Mycobacterial Species by hsp65 Duplex PCR Followed by Duplex-PCR-Based Restriction Analysis and Direct Sequencing

Here we describe a novel duplex PCR method which can differentiate Mycobacterium tuberculosis and nontuberculosis mycobacteria (NTM) strains by amplifying hsp65 DNAs of different sizes (195 and 515 bp, respectively). The devised technique was applied to 54 reference and 170 clinical isolates and dif...

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Hlavní autoři: Kim, Hyun-Ju, Mun, Ho-Suk, Kim, Hong, Oh, Eun-Ju, Ha, Youngju, Bai, Gill-Han, Park, Young-Gil, Cha, Chang-Yong, Kook, Yoon-Hoh, Kim, Bum-Joon
Médium: Článek
Jazyk:English
Vydáno: American Society for Microbiology 2006
Témata:
Mycobacteriology and Aerobic Actinomycetes
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1698348/
https://ncbi.nlm.nih.gov/pubmed/16928964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01214-06
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https://ncbi.nlm.nih.gov/pmc/articles/PMC1698348/
https://ncbi.nlm.nih.gov/pubmed/16928964
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.01214-06

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