An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing.

Three procedures for the removal of immunoglobulin G (IgG) from human serum were evaluated for their effectiveness in eliminating false-positive results caused by rheumatoid factor and in removing IgG from serum to reduce competing-IgG interference in IgM enzyme-linked immunosorbent assay (ELISA) te...

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Main Authors: Martins, T B, Jaskowski, T D, Mouritsen, C L, Hill, H R
Formato: Artigo
Idioma:en
Publicado em: 1995
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC170108/
https://ncbi.nlm.nih.gov/pubmed/7719920
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spelling pubmed-1701082003-08-14 An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing. Martins, T B Jaskowski, T D Mouritsen, C L Hill, H R Clin Diagn Lab Immunol Research Article Three procedures for the removal of immunoglobulin G (IgG) from human serum were evaluated for their effectiveness in eliminating false-positive results caused by rheumatoid factor and in removing IgG from serum to reduce competing-IgG interference in IgM enzyme-linked immunosorbent assay (ELISA) testing. The procedures investigated employed two anti-human IgG diluents and a recombinant protein G-filled tube. The anti-human IgG was more effective than the protein G method in eliminating false-positive results caused by rheumatoid factor and removed 5.4% more IgG from serum samples in the normal range (< 1,700 mg/dl) and up to 16.4% more of the IgG from samples with elevated levels (> 1,700 mg/dl). The recombinant protein G removed less IgM than the anti-human IgG diluents; however, this difference did not affect the results of the ELISA. For these reasons, the in-house-developed anti-human IgG diluent proved to be the most effective and economical for IgM serological testing. 1995-01 /pmc/articles/PMC170108/ /pubmed/7719920 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Martins, T B
Jaskowski, T D
Mouritsen, C L
Hill, H R
An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing.
description Three procedures for the removal of immunoglobulin G (IgG) from human serum were evaluated for their effectiveness in eliminating false-positive results caused by rheumatoid factor and in removing IgG from serum to reduce competing-IgG interference in IgM enzyme-linked immunosorbent assay (ELISA) testing. The procedures investigated employed two anti-human IgG diluents and a recombinant protein G-filled tube. The anti-human IgG was more effective than the protein G method in eliminating false-positive results caused by rheumatoid factor and removed 5.4% more IgG from serum samples in the normal range (< 1,700 mg/dl) and up to 16.4% more of the IgG from samples with elevated levels (> 1,700 mg/dl). The recombinant protein G removed less IgM than the anti-human IgG diluents; however, this difference did not affect the results of the ELISA. For these reasons, the in-house-developed anti-human IgG diluent proved to be the most effective and economical for IgM serological testing.
author Martins, T B
Jaskowski, T D
Mouritsen, C L
Hill, H R
author_facet Martins, T B
Jaskowski, T D
Mouritsen, C L
Hill, H R
author_sort Martins, T B
title An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing.
title_short An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing.
title_full An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing.
title_fullStr An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing.
title_full_unstemmed An evaluation of the effectiveness of three immunoglobulin G (IgG) removal procedures for routine IgM serological testing.
title_sort evaluation of the effectiveness of three immunoglobulin g (igg) removal procedures for routine igm serological testing.
publishDate 1995
url https://ncbi.nlm.nih.gov/pmc/articles/PMC170108/
https://ncbi.nlm.nih.gov/pubmed/7719920
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