An improved method to identify BAC clones using pooled overgos

Hybridization using overgo probes is an established approach for screening arrayed bacterial artificial chromosome (BAC) libraries. We have improved the use of overgos by increasing the yield of positive clones using reduced levels of radioisotopes and enzyme. The strategy involves labeling with all...

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Détails bibliographiques
Auteurs principaux: Madishetty, Kavitha, Condamine, Pascal, Svensson, Jan T., Rodriguez, Edmundo, Close, Timothy J.
Format: Article
Langue:English
Publié: Oxford University Press 2007
Sujets:
Methods Online
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC1761434/
https://ncbi.nlm.nih.gov/pubmed/17151072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl920
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https://ncbi.nlm.nih.gov/pmc/articles/PMC1761434/
https://ncbi.nlm.nih.gov/pubmed/17151072
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkl920

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