Migration of polymorphonuclear leucocytes is influenced by dendritic cells

Dendritic cells (DCs) are the most potent antigen-presenting cells and populate many tissues where they may participate in inflammatory reactions. The infiltration of polymorphonuclear leucocytes (PMNLs) into tissues is a prominent feature of inflammation. The mechanisms of PMNL recruitment depend o...

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Prif Awduron: Scimone, M Lucila, Lutzky, Viviana P, Zittermann, Sandra I, Maffia, Paulo, Jancic, Carolina, Buzzola, Fernanda, Issekutz, Andrew C, Chuluyan, H Eduardo
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Cyhoeddwyd: Blackwell Science Inc 2005
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Original Articles
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC1782099/
https://ncbi.nlm.nih.gov/pubmed/15720439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2567.2005.02104.x
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spelling pubmed-17820992007-02-06 Migration of polymorphonuclear leucocytes is influenced by dendritic cells Scimone, M Lucila Lutzky, Viviana P Zittermann, Sandra I Maffia, Paulo Jancic, Carolina Buzzola, Fernanda Issekutz, Andrew C Chuluyan, H Eduardo Immunology Original Articles Dendritic cells (DCs) are the most potent antigen-presenting cells and populate many tissues where they may participate in inflammatory reactions. The infiltration of polymorphonuclear leucocytes (PMNLs) into tissues is a prominent feature of inflammation. The mechanisms of PMNL recruitment depend on chemotactic factors and adhesion molecules expressed on endothelial cells. The aim of the present study was to determine whether DCs participate in the early recruitment of PMNLs. Dendritic cells derived from peripheral blood monocytes were used for this study. PMNLs incubated with culture supernatant (CS) from untreated or from tumour necrosis factor-α (TNF-α)-treated (1 hr, 100 U/ml, 37°) monocyte-derived DCs (moDCs) had increased surface expression of both CD11b and CD18. Moreover, both untreated and TNF-α-treated moDCs induced PMNL chemotaxis. By blocking CXCL8, CXCL5, CXCL7 and Pan GRO (CXCL1, CXCL2, CXCL3), we observed that CXCL8/interleukin-8 might be the chemokine that induced the PMNL chemotactic activity in the CS of untreated and TNF-α-treated moDC. Furthermore, we investigated the regulation of CXCL8 production in moDCs by adhesion molecule engagement. Our data demonstrated that CD31, CD18, CD29 and CD49d participated in the adhesion of immature moDCs to endothelium. Moreover, engagement of domains 1–3 of CD31, but not of CD29 or CD18, decreased the production of CXCL8 by immature but not mature moDCs (which display lower CD31 levels than immature moDCs). Overall, these results suggest that DCs not only trigger a specific immune response, but also the innate immune response by recruiting PMNLs. Furthermore, our results also suggest that CXCL8 production by immature DCs might be regulated by signalling through CD31 during their migration through the vascular endothelium. Blackwell Science Inc 2005-03 /pmc/articles/PMC1782099/ /pubmed/15720439 http://dx.doi.org/10.1111/j.1365-2567.2005.02104.x Text en © 2005 Blackwell Publishing Ltd
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Original Articles
spellingShingle Original Articles
Scimone, M Lucila
Lutzky, Viviana P
Zittermann, Sandra I
Maffia, Paulo
Jancic, Carolina
Buzzola, Fernanda
Issekutz, Andrew C
Chuluyan, H Eduardo
Migration of polymorphonuclear leucocytes is influenced by dendritic cells
description Dendritic cells (DCs) are the most potent antigen-presenting cells and populate many tissues where they may participate in inflammatory reactions. The infiltration of polymorphonuclear leucocytes (PMNLs) into tissues is a prominent feature of inflammation. The mechanisms of PMNL recruitment depend on chemotactic factors and adhesion molecules expressed on endothelial cells. The aim of the present study was to determine whether DCs participate in the early recruitment of PMNLs. Dendritic cells derived from peripheral blood monocytes were used for this study. PMNLs incubated with culture supernatant (CS) from untreated or from tumour necrosis factor-α (TNF-α)-treated (1 hr, 100 U/ml, 37°) monocyte-derived DCs (moDCs) had increased surface expression of both CD11b and CD18. Moreover, both untreated and TNF-α-treated moDCs induced PMNL chemotaxis. By blocking CXCL8, CXCL5, CXCL7 and Pan GRO (CXCL1, CXCL2, CXCL3), we observed that CXCL8/interleukin-8 might be the chemokine that induced the PMNL chemotactic activity in the CS of untreated and TNF-α-treated moDC. Furthermore, we investigated the regulation of CXCL8 production in moDCs by adhesion molecule engagement. Our data demonstrated that CD31, CD18, CD29 and CD49d participated in the adhesion of immature moDCs to endothelium. Moreover, engagement of domains 1–3 of CD31, but not of CD29 or CD18, decreased the production of CXCL8 by immature but not mature moDCs (which display lower CD31 levels than immature moDCs). Overall, these results suggest that DCs not only trigger a specific immune response, but also the innate immune response by recruiting PMNLs. Furthermore, our results also suggest that CXCL8 production by immature DCs might be regulated by signalling through CD31 during their migration through the vascular endothelium.
author Scimone, M Lucila
Lutzky, Viviana P
Zittermann, Sandra I
Maffia, Paulo
Jancic, Carolina
Buzzola, Fernanda
Issekutz, Andrew C
Chuluyan, H Eduardo
author_facet Scimone, M Lucila
Lutzky, Viviana P
Zittermann, Sandra I
Maffia, Paulo
Jancic, Carolina
Buzzola, Fernanda
Issekutz, Andrew C
Chuluyan, H Eduardo
author_sort Scimone, M Lucila
title Migration of polymorphonuclear leucocytes is influenced by dendritic cells
title_short Migration of polymorphonuclear leucocytes is influenced by dendritic cells
title_full Migration of polymorphonuclear leucocytes is influenced by dendritic cells
title_fullStr Migration of polymorphonuclear leucocytes is influenced by dendritic cells
title_full_unstemmed Migration of polymorphonuclear leucocytes is influenced by dendritic cells
title_sort migration of polymorphonuclear leucocytes is influenced by dendritic cells
publisher Blackwell Science Inc
publisher_facet Blackwell Science Inc
publishDate 2005
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1782099/
https://ncbi.nlm.nih.gov/pubmed/15720439
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2567.2005.02104.x
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