In Vivo Expression of and Cell-Mediated Immune Responses to the Plasmid-Encoded Virulence-Associated Proteins of Rhodococcus equi in Foals

Rhodococcus equi is a facultative intracellular pathogen that causes pneumonia in foals but does not induce disease in adult horses. Virulence of R. equi depends on the presence of a large plasmid, which encodes a family of seven virulence-associated proteins (VapA and VapC to VapH). Eradication of...

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Main Authors: Jacks, Stephanie, Giguère, Steeve, Prescott, John F.
Formato: Artigo
Idioma:English
Publicado em: American Society for Microbiology 2007
Assuntos:
Veterinary Immunology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1865619/
https://ncbi.nlm.nih.gov/pubmed/17301216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00448-06
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spelling pubmed-18656192007-08-01 In Vivo Expression of and Cell-Mediated Immune Responses to the Plasmid-Encoded Virulence-Associated Proteins of Rhodococcus equi in Foals Jacks, Stephanie Giguère, Steeve Prescott, John F. Clin Vaccine Immunol Veterinary Immunology Rhodococcus equi is a facultative intracellular pathogen that causes pneumonia in foals but does not induce disease in adult horses. Virulence of R. equi depends on the presence of a large plasmid, which encodes a family of seven virulence-associated proteins (VapA and VapC to VapH). Eradication of R. equi from the lungs depends on gamma interferon (IFN-γ) production by T lymphocytes. The objectives of the present study were to determine the relative in vivo expression of the vap genes of R. equi in the lungs of infected foals, to determine the recall response of bronchial lymph node (BLN) lymphocytes from foals and adult horses to each of the Vap proteins, and to compare the cytokine profiles of proliferating lymphocytes between foals and adult horses. vapA, vapD, and vapG were preferentially expressed in the lungs of infected foals, and expression of these genes in the lungs was significantly (P < 0.05) higher than that achieved during in vitro growth. VapA and VapC induced the strongest lymphoproliferative responses for foals and adult horses. There was no significant difference in recall lymphoproliferative responses or IFN-γ mRNA expression by bronchial lymph node lymphocytes between foals and adults. In contrast, interleukin 4 (IL-4) expression was significantly higher for adults than for foals for each of the Vap proteins. The ratio of IFN-γ to IL-4 was significantly higher for foals than for adult horses for most Vap proteins. Therefore, foals are immunocompetent and are capable of mounting lymphoproliferative responses of the same magnitude and cytokine phenotype as those of adult horses. American Society for Microbiology 2007-04 2007-02-14 /pmc/articles/PMC1865619/ /pubmed/17301216 http://dx.doi.org/10.1128/CVI.00448-06 Text en Copyright © 2007, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Veterinary Immunology
spellingShingle Veterinary Immunology
Jacks, Stephanie
Giguère, Steeve
Prescott, John F.
In Vivo Expression of and Cell-Mediated Immune Responses to the Plasmid-Encoded Virulence-Associated Proteins of Rhodococcus equi in Foals
description Rhodococcus equi is a facultative intracellular pathogen that causes pneumonia in foals but does not induce disease in adult horses. Virulence of R. equi depends on the presence of a large plasmid, which encodes a family of seven virulence-associated proteins (VapA and VapC to VapH). Eradication of R. equi from the lungs depends on gamma interferon (IFN-γ) production by T lymphocytes. The objectives of the present study were to determine the relative in vivo expression of the vap genes of R. equi in the lungs of infected foals, to determine the recall response of bronchial lymph node (BLN) lymphocytes from foals and adult horses to each of the Vap proteins, and to compare the cytokine profiles of proliferating lymphocytes between foals and adult horses. vapA, vapD, and vapG were preferentially expressed in the lungs of infected foals, and expression of these genes in the lungs was significantly (P < 0.05) higher than that achieved during in vitro growth. VapA and VapC induced the strongest lymphoproliferative responses for foals and adult horses. There was no significant difference in recall lymphoproliferative responses or IFN-γ mRNA expression by bronchial lymph node lymphocytes between foals and adults. In contrast, interleukin 4 (IL-4) expression was significantly higher for adults than for foals for each of the Vap proteins. The ratio of IFN-γ to IL-4 was significantly higher for foals than for adult horses for most Vap proteins. Therefore, foals are immunocompetent and are capable of mounting lymphoproliferative responses of the same magnitude and cytokine phenotype as those of adult horses.
author Jacks, Stephanie
Giguère, Steeve
Prescott, John F.
author_facet Jacks, Stephanie
Giguère, Steeve
Prescott, John F.
author_sort Jacks, Stephanie
title In Vivo Expression of and Cell-Mediated Immune Responses to the Plasmid-Encoded Virulence-Associated Proteins of Rhodococcus equi in Foals
title_short In Vivo Expression of and Cell-Mediated Immune Responses to the Plasmid-Encoded Virulence-Associated Proteins of Rhodococcus equi in Foals
title_full In Vivo Expression of and Cell-Mediated Immune Responses to the Plasmid-Encoded Virulence-Associated Proteins of Rhodococcus equi in Foals
title_fullStr In Vivo Expression of and Cell-Mediated Immune Responses to the Plasmid-Encoded Virulence-Associated Proteins of Rhodococcus equi in Foals
title_full_unstemmed In Vivo Expression of and Cell-Mediated Immune Responses to the Plasmid-Encoded Virulence-Associated Proteins of Rhodococcus equi in Foals
title_sort in vivo expression of and cell-mediated immune responses to the plasmid-encoded virulence-associated proteins of rhodococcus equi in foals
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2007
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1865619/
https://ncbi.nlm.nih.gov/pubmed/17301216
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/CVI.00448-06
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