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Tracking Local Conformational Changes of Ribonuclease A Using Picosecond Time-Resolved Fluorescence of the Six Tyrosine Residues
The six tyrosine residues of ribonuclease A (RNase A) are used as individual intrinsic probes for tracking local conformational changes during unfolding. The fluorescence decays of RNase A are well described by sums of three exponentials with decay times (τ(1) = 1.7 ns, τ(2) = 180 ps, and τ(3) = 30...
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| Hauptverfasser: | , , , , |
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| Format: | Artikel |
| Sprache: | English |
| Veröffentlicht: |
Biophysical Society
2007
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1877784/ https://ncbi.nlm.nih.gov/pubmed/17384067 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.106.093625 |
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