In vitro activity of vancomycin against the spirochete Borrelia burgdorferi.

Borrelia burgdorferi, a spirochete and the causative agent of Lyme disease, has been reported to be susceptible to a variety of antimicrobial agents. In this investigation, the action of vancomycin, a glycopeptide antibiotic not previously known to have activity against spirochetes, against borrelia...

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Main Authors: Dever, L L, Jorgensen, J H, Barbour, A G
Formato: Artigo
Idioma:en
Publicado em: 1993
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC187913/
https://ncbi.nlm.nih.gov/pubmed/8517700
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spelling pubmed-1879132003-12-01 In vitro activity of vancomycin against the spirochete Borrelia burgdorferi. Dever, L L Jorgensen, J H Barbour, A G Antimicrob Agents Chemother Research Article Borrelia burgdorferi, a spirochete and the causative agent of Lyme disease, has been reported to be susceptible to a variety of antimicrobial agents. In this investigation, the action of vancomycin, a glycopeptide antibiotic not previously known to have activity against spirochetes, against borrelias was examined. The in vitro activity of vancomycin against a variety of strains of B. burgdorferi and one strain of Borrelia hermsii was determined by use of a microdilution MIC method (L.L. Dever, J.H. Jorgensen, and A.G. Barbour, J. Clin. Microbiol. 30:2692-2697, 1992). MICs ranged from 0.5 to 2 micrograms/ml. MICs of the glycopeptides ristocetin and teicoplanin and the lipopeptide daptomycin against strain B31 of B. burgdorferi were all > or = 8 micrograms/ml. Subsurface plating, time-kill studies, synergy studies, and electron microscopy were used to investigate further the activity of vancomycin against B31. The MBC of vancomycin was 2 micrograms/ml. Time-kill curves demonstrated > or = 3-log10-unit (99.9%) killing of the final inoculum after 72 h by vancomycin concentrations twice the MIC. Synergy between vancomycin and penicillin was demonstrated at concentrations one-fourth the MIC of each drug. In electron microscopy, B31 cells exposed to vancomycin showed a disruption of cellular integrity and were indistinguishable from those exposed to penicillin. These studies demonstrate another class of microorganisms susceptible in vitro to vancomycin. 1993-05 /pmc/articles/PMC187913/ /pubmed/8517700 Text en
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Research Article
spellingShingle Research Article
Dever, L L
Jorgensen, J H
Barbour, A G
In vitro activity of vancomycin against the spirochete Borrelia burgdorferi.
description Borrelia burgdorferi, a spirochete and the causative agent of Lyme disease, has been reported to be susceptible to a variety of antimicrobial agents. In this investigation, the action of vancomycin, a glycopeptide antibiotic not previously known to have activity against spirochetes, against borrelias was examined. The in vitro activity of vancomycin against a variety of strains of B. burgdorferi and one strain of Borrelia hermsii was determined by use of a microdilution MIC method (L.L. Dever, J.H. Jorgensen, and A.G. Barbour, J. Clin. Microbiol. 30:2692-2697, 1992). MICs ranged from 0.5 to 2 micrograms/ml. MICs of the glycopeptides ristocetin and teicoplanin and the lipopeptide daptomycin against strain B31 of B. burgdorferi were all > or = 8 micrograms/ml. Subsurface plating, time-kill studies, synergy studies, and electron microscopy were used to investigate further the activity of vancomycin against B31. The MBC of vancomycin was 2 micrograms/ml. Time-kill curves demonstrated > or = 3-log10-unit (99.9%) killing of the final inoculum after 72 h by vancomycin concentrations twice the MIC. Synergy between vancomycin and penicillin was demonstrated at concentrations one-fourth the MIC of each drug. In electron microscopy, B31 cells exposed to vancomycin showed a disruption of cellular integrity and were indistinguishable from those exposed to penicillin. These studies demonstrate another class of microorganisms susceptible in vitro to vancomycin.
author Dever, L L
Jorgensen, J H
Barbour, A G
author_facet Dever, L L
Jorgensen, J H
Barbour, A G
author_sort Dever, L L
title In vitro activity of vancomycin against the spirochete Borrelia burgdorferi.
title_short In vitro activity of vancomycin against the spirochete Borrelia burgdorferi.
title_full In vitro activity of vancomycin against the spirochete Borrelia burgdorferi.
title_fullStr In vitro activity of vancomycin against the spirochete Borrelia burgdorferi.
title_full_unstemmed In vitro activity of vancomycin against the spirochete Borrelia burgdorferi.
title_sort in vitro activity of vancomycin against the spirochete borrelia burgdorferi.
publishDate 1993
url https://ncbi.nlm.nih.gov/pmc/articles/PMC187913/
https://ncbi.nlm.nih.gov/pubmed/8517700
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