Beta protein immunoreactivity is found in the majority of neurofibrillary tangles of Alzheimer's disease.

The presence of dystrophic neurites in most extracellular neurofibrillary tangles (E-NFT) suggests a factor promoting neurite growth in E-NFT. Although the beta-protein detected in E-NFT may fill that role, reports that only 2-10% of E-NFT contain beta-protein whereas 80-100% contain dystrophic neur...

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Main Authors: Perry, G., Cras, P., Siedlak, S. L., Tabaton, M., Kawai, M.
Formato: Artigo
Idioma:English
Publicado em: 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1886431/
https://ncbi.nlm.nih.gov/pubmed/1739122
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spelling pubmed-18864312007-06-19 Beta protein immunoreactivity is found in the majority of neurofibrillary tangles of Alzheimer's disease. Perry, G. Cras, P. Siedlak, S. L. Tabaton, M. Kawai, M. Am J Pathol Research Article The presence of dystrophic neurites in most extracellular neurofibrillary tangles (E-NFT) suggests a factor promoting neurite growth in E-NFT. Although the beta-protein detected in E-NFT may fill that role, reports that only 2-10% of E-NFT contain beta-protein whereas 80-100% contain dystrophic neurites suggested that beta-protein does not play an important role. In this study, the authors used two antisera and one monoclonal antibody to beta-protein to establish the effects of tissue preparation and formic acid enhancement on the detection of beta-protein in E-NFT. We found that beta-protein epitopes in E-NFT are sensitive to formaldehyde fixation and are best enhanced by 50% formic acid, whereas beta-protein in senile plaques is best enhanced at higher formic acid concentrations. After treatment with 50% formic acid, beta-protein was found in all E-NFT. Interestingly, after treatment with 10% formic acid, half of intraneuronal-NFT (I-NFT) also contained beta-protein immunoreactivity. The finding that beta-protein immunoreactivity in senile plaques, E-NFT and I-NFT is increased at different formic acid concentrations suggests that beta-protein in each location is in a different conformation. In contrast, no beta-protein immunoreactivity could be found in E-NFT of the brain stem, an area in which dystrophic neurites do not infiltrate E-NFT. These findings indicate a correlation between neuritic infiltration and presence of beta-protein in E-NFT and suggests the two are linked in Alzheimer's disease for E-NFT as well as senile plaques. 1992-02 /pmc/articles/PMC1886431/ /pubmed/1739122 Text en
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Article
spellingShingle Research Article
Perry, G.
Cras, P.
Siedlak, S. L.
Tabaton, M.
Kawai, M.
Beta protein immunoreactivity is found in the majority of neurofibrillary tangles of Alzheimer's disease.
description The presence of dystrophic neurites in most extracellular neurofibrillary tangles (E-NFT) suggests a factor promoting neurite growth in E-NFT. Although the beta-protein detected in E-NFT may fill that role, reports that only 2-10% of E-NFT contain beta-protein whereas 80-100% contain dystrophic neurites suggested that beta-protein does not play an important role. In this study, the authors used two antisera and one monoclonal antibody to beta-protein to establish the effects of tissue preparation and formic acid enhancement on the detection of beta-protein in E-NFT. We found that beta-protein epitopes in E-NFT are sensitive to formaldehyde fixation and are best enhanced by 50% formic acid, whereas beta-protein in senile plaques is best enhanced at higher formic acid concentrations. After treatment with 50% formic acid, beta-protein was found in all E-NFT. Interestingly, after treatment with 10% formic acid, half of intraneuronal-NFT (I-NFT) also contained beta-protein immunoreactivity. The finding that beta-protein immunoreactivity in senile plaques, E-NFT and I-NFT is increased at different formic acid concentrations suggests that beta-protein in each location is in a different conformation. In contrast, no beta-protein immunoreactivity could be found in E-NFT of the brain stem, an area in which dystrophic neurites do not infiltrate E-NFT. These findings indicate a correlation between neuritic infiltration and presence of beta-protein in E-NFT and suggests the two are linked in Alzheimer's disease for E-NFT as well as senile plaques.
author Perry, G.
Cras, P.
Siedlak, S. L.
Tabaton, M.
Kawai, M.
author_facet Perry, G.
Cras, P.
Siedlak, S. L.
Tabaton, M.
Kawai, M.
author_sort Perry, G.
title Beta protein immunoreactivity is found in the majority of neurofibrillary tangles of Alzheimer's disease.
title_short Beta protein immunoreactivity is found in the majority of neurofibrillary tangles of Alzheimer's disease.
title_full Beta protein immunoreactivity is found in the majority of neurofibrillary tangles of Alzheimer's disease.
title_fullStr Beta protein immunoreactivity is found in the majority of neurofibrillary tangles of Alzheimer's disease.
title_full_unstemmed Beta protein immunoreactivity is found in the majority of neurofibrillary tangles of Alzheimer's disease.
title_sort beta protein immunoreactivity is found in the majority of neurofibrillary tangles of alzheimer's disease.
publishDate 1992
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1886431/
https://ncbi.nlm.nih.gov/pubmed/1739122
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