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Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer.
Retroviral vectors are the most efficient tool for stably introducing genes into vertebrate cells. However, their use is limited by the host range of the retrovirus from which they are derived. To alter the host range, we recently constructed retrovirus vector particles, derived from spleen necrosis...
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pubmed-1889502003-10-28 Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. Chu, T H Dornburg, R J Virol Research Article Retroviral vectors are the most efficient tool for stably introducing genes into vertebrate cells. However, their use is limited by the host range of the retrovirus from which they are derived. To alter the host range, we recently constructed retrovirus vector particles, derived from spleen necrosis virus, that display a single-chain antigen-binding site of an antibody (scA) on the viral surface (T.-H. T. Chu, I. Martinez, W. Sheay, and R. Dornburg, Gene Ther. 1:292-299, 1994). Using a hapten (2,4-dinitrophenol) model system, we showed that such particles are competent for infection. In this study, we repeated our experiments using an scA directed against a cell surface protein expressed on various human carcinoma cell lines. We found that such scA-displaying particles can efficiently infect human cells that express the corresponding antigen. Particles with wild-type spleen necrosis virus envelope are minimally infectious on such cells. The addition of the original monoclonal antibody to the viral vector particle solution prior to infection inhibited infection. This competition assay showed that the infection is mediated by the antibody moiety and, therefore, is antibody specific. These data indicate that retroviral vectors with antibody-envelope fusion proteins may be a valuable tool for selectively introducing genes into any target cell. 1995-04 /pmc/articles/PMC188950/ /pubmed/7884919 Text en |
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US National Library of Medicine |
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PubMed Central |
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Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Correction Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article |
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Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Correction Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Research Article Chu, T H Dornburg, R Berinstein, A Roivainen, M Hovi, T Mason, P W Baxt, B Roberts, S R Compans, R W Wertz, G W Cohrs, R J Barbour, M B Mahalingam, R Wellish, M Gilden, D H Godeny, E K Zeng, L Smith, S L Brinton, M A Yokoyama, M Zhang, J Whitton, J L Rao, A L Grantham, G L Corsini, J Carlson, J O Maxwell, F Maxwell, I H Hahn, H Palmenberg, A C Kaverin, N V Webster, R G von Weizsäcker, F Wieland, S Blum, H E Wang, N Zhu, T Ho, D D Brandsma, J L Brownstein, D G Xiao, W Longley, B J Ball, L A Castrucci, M R Kawaoka, Y Engelman, A Englund, G Orenstein, J M Martin, M A Craigie, R Clements, J E Montelaro, R C Zink, M C Amedee, A M Miller, S Trichel, A M Jagerski, B Hauer, D Martin, L N Bohm, R P Bernstein, H B Tucker, S P Kar, S R McPherson, S A McPherson, D T Dubay, J W Lebowitz, J Compans, R W Hunter, E Rosé, J R Babé, L M Craik, C S Kondo, E Mammano, F Cohen, E A Göttlinger, H G d'Adda di Fagagna, F Marzio, G Gutierrez, M I Kang, L Y Falaschi, A Giacca, M Milich, D R Peterson, D L Schödel, F Jones, J E Hughes, J L Zhao, Y Holden, V R Smith, R H O'Callaghan, D J Courtois, G Bénit, L Mikaeloff, Y Pauchard, M Charon, M Varlet, P Gisselbrecht, S Choe, H R Sodroski, J Berthomme, H Monahan, S J Parris, D S Jacquemont, B Epstein, A L Meyer, K Petersen, A Niepmann, M Beck, E Yu, D Wang, C C Wang, A L Monastyrskaya, K Gould, E A Roy, P Rose, P E Schaffhausen, B S Stevenson, S C Rollence, M White, B Weaver, L McClelland, A Corapi, W V Darteil, R J Audonnet, J C Chappuis, G E Wattel, E Vartanian, J P Pannetier, C Wain-Hobson, S Novella, I S Elena, S F Moya, A Domingo, E Holland, J J Wang, K Krause, P R Straus, S E Lichtenstein, D L Rushlow, K E Cook, R F Raabe, M L Swardson, C J Kociba, G J Issel, C J Montelaro, R C Bohenzky, R A Lagunoff, M Roizman, B Wagner, E K Silverstein, S Zaiman, A L Lewis, A F Crute, B E Speck, N A Lenz, J Song, C Z Loewenstein, P M Green, M Xu, B Yoo, S Guarino, L A Cane, P A Pringle, C R Qiao, X Casini, G Qiao, J Mindich, L Tan, W Schwartz, S Stillman, E A Rose, J K Whitt, M A Roehl, H H Semler, B L Dorsman, J C Hagmeyer, B M Veenstra, J Elfferich, P Nabben, N Zantema, A van der Eb, A J Mitchell, T Sugden, B Spina, C A Guatelli, J C Richman, D D Jeon, S Allen-Hoffmann, B L Lambert, P F Fixman, E D Hayward, G S Hayward, S D Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. |
| description |
Retroviral vectors are the most efficient tool for stably introducing genes into vertebrate cells. However, their use is limited by the host range of the retrovirus from which they are derived. To alter the host range, we recently constructed retrovirus vector particles, derived from spleen necrosis virus, that display a single-chain antigen-binding site of an antibody (scA) on the viral surface (T.-H. T. Chu, I. Martinez, W. Sheay, and R. Dornburg, Gene Ther. 1:292-299, 1994). Using a hapten (2,4-dinitrophenol) model system, we showed that such particles are competent for infection. In this study, we repeated our experiments using an scA directed against a cell surface protein expressed on various human carcinoma cell lines. We found that such scA-displaying particles can efficiently infect human cells that express the corresponding antigen. Particles with wild-type spleen necrosis virus envelope are minimally infectious on such cells. The addition of the original monoclonal antibody to the viral vector particle solution prior to infection inhibited infection. This competition assay showed that the infection is mediated by the antibody moiety and, therefore, is antibody specific. These data indicate that retroviral vectors with antibody-envelope fusion proteins may be a valuable tool for selectively introducing genes into any target cell. |
| author |
Chu, T H Dornburg, R Berinstein, A Roivainen, M Hovi, T Mason, P W Baxt, B Roberts, S R Compans, R W Wertz, G W Cohrs, R J Barbour, M B Mahalingam, R Wellish, M Gilden, D H Godeny, E K Zeng, L Smith, S L Brinton, M A Yokoyama, M Zhang, J Whitton, J L Rao, A L Grantham, G L Corsini, J Carlson, J O Maxwell, F Maxwell, I H Hahn, H Palmenberg, A C Kaverin, N V Webster, R G von Weizsäcker, F Wieland, S Blum, H E Wang, N Zhu, T Ho, D D Brandsma, J L Brownstein, D G Xiao, W Longley, B J Ball, L A Castrucci, M R Kawaoka, Y Engelman, A Englund, G Orenstein, J M Martin, M A Craigie, R Clements, J E Montelaro, R C Zink, M C Amedee, A M Miller, S Trichel, A M Jagerski, B Hauer, D Martin, L N Bohm, R P Bernstein, H B Tucker, S P Kar, S R McPherson, S A McPherson, D T Dubay, J W Lebowitz, J Compans, R W Hunter, E Rosé, J R Babé, L M Craik, C S Kondo, E Mammano, F Cohen, E A Göttlinger, H G d'Adda di Fagagna, F Marzio, G Gutierrez, M I Kang, L Y Falaschi, A Giacca, M Milich, D R Peterson, D L Schödel, F Jones, J E Hughes, J L Zhao, Y Holden, V R Smith, R H O'Callaghan, D J Courtois, G Bénit, L Mikaeloff, Y Pauchard, M Charon, M Varlet, P Gisselbrecht, S Choe, H R Sodroski, J Berthomme, H Monahan, S J Parris, D S Jacquemont, B Epstein, A L Meyer, K Petersen, A Niepmann, M Beck, E Yu, D Wang, C C Wang, A L Monastyrskaya, K Gould, E A Roy, P Rose, P E Schaffhausen, B S Stevenson, S C Rollence, M White, B Weaver, L McClelland, A Corapi, W V Darteil, R J Audonnet, J C Chappuis, G E Wattel, E Vartanian, J P Pannetier, C Wain-Hobson, S Novella, I S Elena, S F Moya, A Domingo, E Holland, J J Wang, K Krause, P R Straus, S E Lichtenstein, D L Rushlow, K E Cook, R F Raabe, M L Swardson, C J Kociba, G J Issel, C J Montelaro, R C Bohenzky, R A Lagunoff, M Roizman, B Wagner, E K Silverstein, S Zaiman, A L Lewis, A F Crute, B E Speck, N A Lenz, J Song, C Z Loewenstein, P M Green, M Xu, B Yoo, S Guarino, L A Cane, P A Pringle, C R Qiao, X Casini, G Qiao, J Mindich, L Tan, W Schwartz, S Stillman, E A Rose, J K Whitt, M A Roehl, H H Semler, B L Dorsman, J C Hagmeyer, B M Veenstra, J Elfferich, P Nabben, N Zantema, A van der Eb, A J Mitchell, T Sugden, B Spina, C A Guatelli, J C Richman, D D Jeon, S Allen-Hoffmann, B L Lambert, P F Fixman, E D Hayward, G S Hayward, S D |
| author_facet |
Chu, T H Dornburg, R Berinstein, A Roivainen, M Hovi, T Mason, P W Baxt, B Roberts, S R Compans, R W Wertz, G W Cohrs, R J Barbour, M B Mahalingam, R Wellish, M Gilden, D H Godeny, E K Zeng, L Smith, S L Brinton, M A Yokoyama, M Zhang, J Whitton, J L Rao, A L Grantham, G L Corsini, J Carlson, J O Maxwell, F Maxwell, I H Hahn, H Palmenberg, A C Kaverin, N V Webster, R G von Weizsäcker, F Wieland, S Blum, H E Wang, N Zhu, T Ho, D D Brandsma, J L Brownstein, D G Xiao, W Longley, B J Ball, L A Castrucci, M R Kawaoka, Y Engelman, A Englund, G Orenstein, J M Martin, M A Craigie, R Clements, J E Montelaro, R C Zink, M C Amedee, A M Miller, S Trichel, A M Jagerski, B Hauer, D Martin, L N Bohm, R P Bernstein, H B Tucker, S P Kar, S R McPherson, S A McPherson, D T Dubay, J W Lebowitz, J Compans, R W Hunter, E Rosé, J R Babé, L M Craik, C S Kondo, E Mammano, F Cohen, E A Göttlinger, H G d'Adda di Fagagna, F Marzio, G Gutierrez, M I Kang, L Y Falaschi, A Giacca, M Milich, D R Peterson, D L Schödel, F Jones, J E Hughes, J L Zhao, Y Holden, V R Smith, R H O'Callaghan, D J Courtois, G Bénit, L Mikaeloff, Y Pauchard, M Charon, M Varlet, P Gisselbrecht, S Choe, H R Sodroski, J Berthomme, H Monahan, S J Parris, D S Jacquemont, B Epstein, A L Meyer, K Petersen, A Niepmann, M Beck, E Yu, D Wang, C C Wang, A L Monastyrskaya, K Gould, E A Roy, P Rose, P E Schaffhausen, B S Stevenson, S C Rollence, M White, B Weaver, L McClelland, A Corapi, W V Darteil, R J Audonnet, J C Chappuis, G E Wattel, E Vartanian, J P Pannetier, C Wain-Hobson, S Novella, I S Elena, S F Moya, A Domingo, E Holland, J J Wang, K Krause, P R Straus, S E Lichtenstein, D L Rushlow, K E Cook, R F Raabe, M L Swardson, C J Kociba, G J Issel, C J Montelaro, R C Bohenzky, R A Lagunoff, M Roizman, B Wagner, E K Silverstein, S Zaiman, A L Lewis, A F Crute, B E Speck, N A Lenz, J Song, C Z Loewenstein, P M Green, M Xu, B Yoo, S Guarino, L A Cane, P A Pringle, C R Qiao, X Casini, G Qiao, J Mindich, L Tan, W Schwartz, S Stillman, E A Rose, J K Whitt, M A Roehl, H H Semler, B L Dorsman, J C Hagmeyer, B M Veenstra, J Elfferich, P Nabben, N Zantema, A van der Eb, A J Mitchell, T Sugden, B Spina, C A Guatelli, J C Richman, D D Jeon, S Allen-Hoffmann, B L Lambert, P F Fixman, E D Hayward, G S Hayward, S D |
| author_sort |
Chu, T H |
| title |
Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. |
| title_short |
Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. |
| title_full |
Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. |
| title_fullStr |
Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. |
| title_full_unstemmed |
Retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. |
| title_sort |
retroviral vector particles displaying the antigen-binding site of an antibody enable cell-type-specific gene transfer. |
| publishDate |
1995 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC188950/ https://ncbi.nlm.nih.gov/pubmed/7884919 https://ncbi.nlm.nih.gov/pmc/articles/PMC188951/ https://ncbi.nlm.nih.gov/pubmed/7533862 https://ncbi.nlm.nih.gov/pmc/articles/PMC188952/ https://ncbi.nlm.nih.gov/pubmed/7884920 https://ncbi.nlm.nih.gov/pmc/articles/PMC188953/ https://ncbi.nlm.nih.gov/pubmed/7884921 https://ncbi.nlm.nih.gov/pmc/articles/PMC188954/ https://ncbi.nlm.nih.gov/pubmed/7884922 https://ncbi.nlm.nih.gov/pmc/articles/PMC188955/ https://ncbi.nlm.nih.gov/pubmed/7884923 https://ncbi.nlm.nih.gov/pmc/articles/PMC188956/ https://ncbi.nlm.nih.gov/pubmed/7884924 https://ncbi.nlm.nih.gov/pmc/articles/PMC188957/ https://ncbi.nlm.nih.gov/pubmed/7884925 https://ncbi.nlm.nih.gov/pmc/articles/PMC188958/ https://ncbi.nlm.nih.gov/pubmed/7884926 https://ncbi.nlm.nih.gov/pmc/articles/PMC188959/ https://ncbi.nlm.nih.gov/pubmed/7884927 https://ncbi.nlm.nih.gov/pmc/articles/PMC188960/ https://ncbi.nlm.nih.gov/pubmed/7884928 https://ncbi.nlm.nih.gov/pmc/articles/PMC188961/ https://ncbi.nlm.nih.gov/pubmed/7884929 https://ncbi.nlm.nih.gov/pmc/articles/PMC188962/ https://ncbi.nlm.nih.gov/pubmed/7884930 https://ncbi.nlm.nih.gov/pmc/articles/PMC188963/ https://ncbi.nlm.nih.gov/pubmed/7884931 https://ncbi.nlm.nih.gov/pmc/articles/PMC188964/ https://ncbi.nlm.nih.gov/pubmed/7535862 https://ncbi.nlm.nih.gov/pmc/articles/PMC188965/ https://ncbi.nlm.nih.gov/pubmed/7535863 https://ncbi.nlm.nih.gov/pmc/articles/PMC188966/ https://ncbi.nlm.nih.gov/pubmed/7707496 https://ncbi.nlm.nih.gov/pmc/articles/PMC188967/ https://ncbi.nlm.nih.gov/pubmed/7707497 https://ncbi.nlm.nih.gov/pmc/articles/PMC188968/ https://ncbi.nlm.nih.gov/pubmed/7535864 https://ncbi.nlm.nih.gov/pmc/articles/PMC188969/ https://ncbi.nlm.nih.gov/pubmed/7707498 https://ncbi.nlm.nih.gov/pmc/articles/PMC188970/ https://ncbi.nlm.nih.gov/pubmed/7707499 https://ncbi.nlm.nih.gov/pmc/articles/PMC188971/ https://ncbi.nlm.nih.gov/pubmed/7535865 https://ncbi.nlm.nih.gov/pmc/articles/PMC188972/ https://ncbi.nlm.nih.gov/pubmed/7707500 https://ncbi.nlm.nih.gov/pmc/articles/PMC188973/ https://ncbi.nlm.nih.gov/pubmed/7707501 https://ncbi.nlm.nih.gov/pmc/articles/PMC188974/ https://ncbi.nlm.nih.gov/pubmed/7707502 https://ncbi.nlm.nih.gov/pmc/articles/PMC188975/ https://ncbi.nlm.nih.gov/pubmed/7707503 https://ncbi.nlm.nih.gov/pmc/articles/PMC188976/ https://ncbi.nlm.nih.gov/pubmed/7707504 https://ncbi.nlm.nih.gov/pmc/articles/PMC188977/ https://ncbi.nlm.nih.gov/pubmed/7707505 https://ncbi.nlm.nih.gov/pmc/articles/PMC188978/ https://ncbi.nlm.nih.gov/pubmed/7535866 https://ncbi.nlm.nih.gov/pmc/articles/PMC188979/ https://ncbi.nlm.nih.gov/pubmed/7707506 https://ncbi.nlm.nih.gov/pmc/articles/PMC188980/ https://ncbi.nlm.nih.gov/pubmed/7707507 https://ncbi.nlm.nih.gov/pmc/articles/PMC188981/ https://ncbi.nlm.nih.gov/pubmed/7707508 https://ncbi.nlm.nih.gov/pmc/articles/PMC188982/ https://ncbi.nlm.nih.gov/pubmed/7707509 https://ncbi.nlm.nih.gov/pmc/articles/PMC188983/ https://ncbi.nlm.nih.gov/pubmed/7707510 https://ncbi.nlm.nih.gov/pmc/articles/PMC188984/ https://ncbi.nlm.nih.gov/pubmed/7707511 https://ncbi.nlm.nih.gov/pmc/articles/PMC188985/ https://ncbi.nlm.nih.gov/pubmed/7707512 https://ncbi.nlm.nih.gov/pmc/articles/PMC188986/ https://ncbi.nlm.nih.gov/pubmed/7707513 https://ncbi.nlm.nih.gov/pmc/articles/PMC188987/ https://ncbi.nlm.nih.gov/pubmed/7707514 https://ncbi.nlm.nih.gov/pmc/articles/PMC188988/ https://ncbi.nlm.nih.gov/pubmed/7707515 https://ncbi.nlm.nih.gov/pmc/articles/PMC188989/ https://ncbi.nlm.nih.gov/pubmed/7707516 https://ncbi.nlm.nih.gov/pmc/articles/PMC188990/ https://ncbi.nlm.nih.gov/pubmed/7707517 https://ncbi.nlm.nih.gov/pmc/articles/PMC188991/ https://ncbi.nlm.nih.gov/pubmed/7707518 https://ncbi.nlm.nih.gov/pmc/articles/PMC188992/ https://ncbi.nlm.nih.gov/pubmed/7707519 https://ncbi.nlm.nih.gov/pmc/articles/PMC188993/ https://ncbi.nlm.nih.gov/pubmed/7707520 https://ncbi.nlm.nih.gov/pmc/articles/PMC188994/ https://ncbi.nlm.nih.gov/pubmed/7707521 https://ncbi.nlm.nih.gov/pmc/articles/PMC188995/ https://ncbi.nlm.nih.gov/pubmed/7707522 https://ncbi.nlm.nih.gov/pmc/articles/PMC188996/ https://ncbi.nlm.nih.gov/pubmed/7707523 https://ncbi.nlm.nih.gov/pmc/articles/PMC188997/ https://ncbi.nlm.nih.gov/pubmed/7707524 https://ncbi.nlm.nih.gov/pmc/articles/PMC188998/ https://ncbi.nlm.nih.gov/pubmed/7707525 https://ncbi.nlm.nih.gov/pmc/articles/PMC188999/ https://ncbi.nlm.nih.gov/pubmed/7707526 |
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