Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression

Escherichia coli strain MG1655 was chosen for sequencing because the few mutations it carries (ilvG rfb-50 rph-1) were considered innocuous. However, it has a number of growth defects. Internal pyrimidine starvation due to polarity of the rph-1 allele on pyrE was problematic in continuous culture. M...

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Prif Awduron: Soupene, Eric, van Heeswijk, Wally C., Plumbridge, Jacqueline, Stewart, Valley, Bertenthal, Daniel, Lee, Haidy, Prasad, Gyaneshwar, Paliy, Oleg, Charernnoppakul, Parinya, Kustu, Sydney
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Cyhoeddwyd: American Society for Microbiology 2003
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Genomics and Proteomics
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC193769/
https://ncbi.nlm.nih.gov/pubmed/12949114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.18.5611-5626.2003
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spelling pubmed-1937692003-11-14 Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression Soupene, Eric van Heeswijk, Wally C. Plumbridge, Jacqueline Stewart, Valley Bertenthal, Daniel Lee, Haidy Prasad, Gyaneshwar Paliy, Oleg Charernnoppakul, Parinya Kustu, Sydney J Bacteriol Genomics and Proteomics Escherichia coli strain MG1655 was chosen for sequencing because the few mutations it carries (ilvG rfb-50 rph-1) were considered innocuous. However, it has a number of growth defects. Internal pyrimidine starvation due to polarity of the rph-1 allele on pyrE was problematic in continuous culture. Moreover, the isolate of MG1655 obtained from the E. coli Genetic Stock Center also carries a large deletion around the fnr (fumarate-nitrate respiration) regulatory gene. Although studies on DNA microarrays revealed apparent cross-regulation of gene expression between galactose and lactose metabolism in the Stock Center isolate of MG1655, this was due to the occurrence of mutations that increased lacY expression and suppressed slow growth on galactose. The explanation for apparent cross-regulation between galactose and N-acetylglucosamine metabolism was similar. By contrast, cross-regulation between lactose and maltose metabolism appeared to be due to generation of internal maltosaccharides in lactose-grown cells and may be physiologically significant. Lactose is of restricted distribution: it is normally found together with maltosaccharides, which are starch degradation products, in the mammalian intestine. Strains designated MG1655 and obtained from other sources differed from the Stock Center isolate and each other in several respects. We confirmed that use of other E. coli strains with MG1655-based DNA microarrays works well, and hence these arrays can be used to study any strain of interest. The responses to nitrogen limitation of two urinary tract isolates and an intestinal commensal strain isolated recently from humans were remarkably similar to those of MG1655. American Society for Microbiology 2003-09 /pmc/articles/PMC193769/ /pubmed/12949114 http://dx.doi.org/10.1128/JB.185.18.5611-5626.2003 Text en Copyright © 2003, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Genomics and Proteomics
spellingShingle Genomics and Proteomics
Soupene, Eric
van Heeswijk, Wally C.
Plumbridge, Jacqueline
Stewart, Valley
Bertenthal, Daniel
Lee, Haidy
Prasad, Gyaneshwar
Paliy, Oleg
Charernnoppakul, Parinya
Kustu, Sydney
Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression
description Escherichia coli strain MG1655 was chosen for sequencing because the few mutations it carries (ilvG rfb-50 rph-1) were considered innocuous. However, it has a number of growth defects. Internal pyrimidine starvation due to polarity of the rph-1 allele on pyrE was problematic in continuous culture. Moreover, the isolate of MG1655 obtained from the E. coli Genetic Stock Center also carries a large deletion around the fnr (fumarate-nitrate respiration) regulatory gene. Although studies on DNA microarrays revealed apparent cross-regulation of gene expression between galactose and lactose metabolism in the Stock Center isolate of MG1655, this was due to the occurrence of mutations that increased lacY expression and suppressed slow growth on galactose. The explanation for apparent cross-regulation between galactose and N-acetylglucosamine metabolism was similar. By contrast, cross-regulation between lactose and maltose metabolism appeared to be due to generation of internal maltosaccharides in lactose-grown cells and may be physiologically significant. Lactose is of restricted distribution: it is normally found together with maltosaccharides, which are starch degradation products, in the mammalian intestine. Strains designated MG1655 and obtained from other sources differed from the Stock Center isolate and each other in several respects. We confirmed that use of other E. coli strains with MG1655-based DNA microarrays works well, and hence these arrays can be used to study any strain of interest. The responses to nitrogen limitation of two urinary tract isolates and an intestinal commensal strain isolated recently from humans were remarkably similar to those of MG1655.
author Soupene, Eric
van Heeswijk, Wally C.
Plumbridge, Jacqueline
Stewart, Valley
Bertenthal, Daniel
Lee, Haidy
Prasad, Gyaneshwar
Paliy, Oleg
Charernnoppakul, Parinya
Kustu, Sydney
author_facet Soupene, Eric
van Heeswijk, Wally C.
Plumbridge, Jacqueline
Stewart, Valley
Bertenthal, Daniel
Lee, Haidy
Prasad, Gyaneshwar
Paliy, Oleg
Charernnoppakul, Parinya
Kustu, Sydney
author_sort Soupene, Eric
title Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression
title_short Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression
title_full Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression
title_fullStr Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression
title_full_unstemmed Physiological Studies of Escherichia coli Strain MG1655: Growth Defects and Apparent Cross-Regulation of Gene Expression
title_sort physiological studies of escherichia coli strain mg1655: growth defects and apparent cross-regulation of gene expression
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2003
url https://ncbi.nlm.nih.gov/pmc/articles/PMC193769/
https://ncbi.nlm.nih.gov/pubmed/12949114
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JB.185.18.5611-5626.2003
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