Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination

West Nile virus (WNV) is an emerging flavivirus that has caused frequent epidemics since 1996. Besides natural transmission by mosquitoes, WNV can also be transmitted through blood transfusion and organ transplantation, thus heightening the urgency of development of a specific and rapid serologic as...

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Автори: Wong, Susan J., Boyle, Rebekah H., Demarest, Valerie L., Woodmansee, Anh N., Kramer, Laura D., Li, Hongmin, Drebot, Michael, Koski, Raymond A., Fikrig, Erol, Martin, Denise A., Shi, Pei-Yong
Формат: Стаття
Мова:en
Опубліковано: American Society for Microbiology 2003
Предмети:
Virology
Онлайн доступ:https://ncbi.nlm.nih.gov/pmc/articles/PMC193845/
https://ncbi.nlm.nih.gov/pubmed/12958248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.9.4217-4223.2003
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spelling pubmed-1938452003-11-14 Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination Wong, Susan J. Boyle, Rebekah H. Demarest, Valerie L. Woodmansee, Anh N. Kramer, Laura D. Li, Hongmin Drebot, Michael Koski, Raymond A. Fikrig, Erol Martin, Denise A. Shi, Pei-Yong J Clin Microbiol Virology West Nile virus (WNV) is an emerging flavivirus that has caused frequent epidemics since 1996. Besides natural transmission by mosquitoes, WNV can also be transmitted through blood transfusion and organ transplantation, thus heightening the urgency of development of a specific and rapid serologic assay of WNV infection. The current immunoassays lack specificity because they are based on detection of antibodies against WNV structural proteins and immune responses to structural proteins among flaviviruses cross-react to each other. Here, we describe microsphere immunoassays that detect antibodies to nonstructural proteins 3 and 5 (NS3 and NS5). In contrast to immunoassays based on viral envelope and NS3 proteins, the NS5-based assay (i) reliably discriminates between WNV infections and dengue virus or St. Louis encephalitis virus infections, (ii) differentiates between flavivirus vaccination and natural WNV infection, and (iii) indicates recent infections. These unique features of the NS5-based immunoassay will be very useful for both clinical and veterinary diagnosis of WNV infection. American Society for Microbiology 2003-09 /pmc/articles/PMC193845/ /pubmed/12958248 http://dx.doi.org/10.1128/JCM.41.9.4217-4223.2003 Text en Copyright © 2003, American Society for Microbiology
institution US National Library of Medicine
collection PubMed Central
language en
format Article
topic Virology
spellingShingle Virology
Wong, Susan J.
Boyle, Rebekah H.
Demarest, Valerie L.
Woodmansee, Anh N.
Kramer, Laura D.
Li, Hongmin
Drebot, Michael
Koski, Raymond A.
Fikrig, Erol
Martin, Denise A.
Shi, Pei-Yong
Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination
description West Nile virus (WNV) is an emerging flavivirus that has caused frequent epidemics since 1996. Besides natural transmission by mosquitoes, WNV can also be transmitted through blood transfusion and organ transplantation, thus heightening the urgency of development of a specific and rapid serologic assay of WNV infection. The current immunoassays lack specificity because they are based on detection of antibodies against WNV structural proteins and immune responses to structural proteins among flaviviruses cross-react to each other. Here, we describe microsphere immunoassays that detect antibodies to nonstructural proteins 3 and 5 (NS3 and NS5). In contrast to immunoassays based on viral envelope and NS3 proteins, the NS5-based assay (i) reliably discriminates between WNV infections and dengue virus or St. Louis encephalitis virus infections, (ii) differentiates between flavivirus vaccination and natural WNV infection, and (iii) indicates recent infections. These unique features of the NS5-based immunoassay will be very useful for both clinical and veterinary diagnosis of WNV infection.
author Wong, Susan J.
Boyle, Rebekah H.
Demarest, Valerie L.
Woodmansee, Anh N.
Kramer, Laura D.
Li, Hongmin
Drebot, Michael
Koski, Raymond A.
Fikrig, Erol
Martin, Denise A.
Shi, Pei-Yong
author_facet Wong, Susan J.
Boyle, Rebekah H.
Demarest, Valerie L.
Woodmansee, Anh N.
Kramer, Laura D.
Li, Hongmin
Drebot, Michael
Koski, Raymond A.
Fikrig, Erol
Martin, Denise A.
Shi, Pei-Yong
author_sort Wong, Susan J.
title Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination
title_short Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination
title_full Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination
title_fullStr Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination
title_full_unstemmed Immunoassay Targeting Nonstructural Protein 5 To Differentiate West Nile Virus Infection from Dengue and St. Louis Encephalitis Virus Infections and from Flavivirus Vaccination
title_sort immunoassay targeting nonstructural protein 5 to differentiate west nile virus infection from dengue and st. louis encephalitis virus infections and from flavivirus vaccination
publisher American Society for Microbiology
publisher_facet American Society for Microbiology
publishDate 2003
url https://ncbi.nlm.nih.gov/pmc/articles/PMC193845/
https://ncbi.nlm.nih.gov/pubmed/12958248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.9.4217-4223.2003
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