Long-term T cell memory to human leucocyte antigen-A2 supertype epitopes in humans vaccinated against smallpox

Identification of human leucocyte antigen (HLA) class I-restricted T cell epitopes is important to develop methods to track the evolution of T cell memory to new generation smallpox vaccines and allow comparison to older vaccinia virus preparations known to induce protection against smallpox. We eva...

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Autors principals: Ostrout, N D, McHugh, M M, Tisch, D J, Moormann, A M, Brusic, V, Kazura, J W
Format: Article
Idioma:English
Publicat: Blackwell Science Inc 2007
Matèries:
Translational Studies
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1941954/
https://ncbi.nlm.nih.gov/pubmed/17488297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2249.2007.03401.x
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spelling pubmed-19419542008-08-01 Long-term T cell memory to human leucocyte antigen-A2 supertype epitopes in humans vaccinated against smallpox Ostrout, N D McHugh, M M Tisch, D J Moormann, A M Brusic, V Kazura, J W Clin Exp Immunol Translational Studies Identification of human leucocyte antigen (HLA) class I-restricted T cell epitopes is important to develop methods to track the evolution of T cell memory to new generation smallpox vaccines and allow comparison to older vaccinia virus preparations known to induce protection against smallpox. We evaluated the relative predictive values of four computational algorithms to identify candidate 9-mer HLA-A2 supertype epitopes that were confirmed to stimulate preferentially T cell interferon (IFN)-γ responses by subjects last vaccinated with Dryvax 27–54 years previously. Six peptides encoded by I4L, G1L, A8R, I8R, D12L and H3L open reading frames that were identical for Vaccinia (Copenhagen), Variola major (Bangledesh 1975) and modified vaccinia Ankara strain preferentially stimulated IFN-γ responses by healthy HLA-A2 supertype adults last given Dryvax 27–49 years earlier relative to remotely vaccinated non-HLA-A2 supertype and unvaccinated HLA-A2 supertype adults. Combining results from at least two computational algorithms that use different strategies to predict peptide binding to HLA-A2 supertype molecules was optimal for selection of candidate peptides that were confirmed to be epitopes by recall of T cell IFN-γ responses. These data will facilitate evaluation of the immunogenicity of replication incompetent smallpox vaccines such as modified vaccinia Ankara and contribute to knowledge of poxvirus epitopes that are associated with long-lived T cell memory. Blackwell Science Inc 2007-08 /pmc/articles/PMC1941954/ /pubmed/17488297 http://dx.doi.org/10.1111/j.1365-2249.2007.03401.x Text en © 2007 British Society for Immunology
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Translational Studies
spellingShingle Translational Studies
Ostrout, N D
McHugh, M M
Tisch, D J
Moormann, A M
Brusic, V
Kazura, J W
Long-term T cell memory to human leucocyte antigen-A2 supertype epitopes in humans vaccinated against smallpox
description Identification of human leucocyte antigen (HLA) class I-restricted T cell epitopes is important to develop methods to track the evolution of T cell memory to new generation smallpox vaccines and allow comparison to older vaccinia virus preparations known to induce protection against smallpox. We evaluated the relative predictive values of four computational algorithms to identify candidate 9-mer HLA-A2 supertype epitopes that were confirmed to stimulate preferentially T cell interferon (IFN)-γ responses by subjects last vaccinated with Dryvax 27–54 years previously. Six peptides encoded by I4L, G1L, A8R, I8R, D12L and H3L open reading frames that were identical for Vaccinia (Copenhagen), Variola major (Bangledesh 1975) and modified vaccinia Ankara strain preferentially stimulated IFN-γ responses by healthy HLA-A2 supertype adults last given Dryvax 27–49 years earlier relative to remotely vaccinated non-HLA-A2 supertype and unvaccinated HLA-A2 supertype adults. Combining results from at least two computational algorithms that use different strategies to predict peptide binding to HLA-A2 supertype molecules was optimal for selection of candidate peptides that were confirmed to be epitopes by recall of T cell IFN-γ responses. These data will facilitate evaluation of the immunogenicity of replication incompetent smallpox vaccines such as modified vaccinia Ankara and contribute to knowledge of poxvirus epitopes that are associated with long-lived T cell memory.
author Ostrout, N D
McHugh, M M
Tisch, D J
Moormann, A M
Brusic, V
Kazura, J W
author_facet Ostrout, N D
McHugh, M M
Tisch, D J
Moormann, A M
Brusic, V
Kazura, J W
author_sort Ostrout, N D
title Long-term T cell memory to human leucocyte antigen-A2 supertype epitopes in humans vaccinated against smallpox
title_short Long-term T cell memory to human leucocyte antigen-A2 supertype epitopes in humans vaccinated against smallpox
title_full Long-term T cell memory to human leucocyte antigen-A2 supertype epitopes in humans vaccinated against smallpox
title_fullStr Long-term T cell memory to human leucocyte antigen-A2 supertype epitopes in humans vaccinated against smallpox
title_full_unstemmed Long-term T cell memory to human leucocyte antigen-A2 supertype epitopes in humans vaccinated against smallpox
title_sort long-term t cell memory to human leucocyte antigen-a2 supertype epitopes in humans vaccinated against smallpox
publisher Blackwell Science Inc
publisher_facet Blackwell Science Inc
publishDate 2007
url https://ncbi.nlm.nih.gov/pmc/articles/PMC1941954/
https://ncbi.nlm.nih.gov/pubmed/17488297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/j.1365-2249.2007.03401.x
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