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Rapid isolation of cDNA by hybridization
The isolation of genes from a given genomic region can be a rate-limiting step in the discovery of disease genes. We describe an approach to the isolation of cDNAs that have sequences in common with large genomic clones such as bacterial artificial chromosomes. We applied this method to loci both am...
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The National Academy of Sciences
1998
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| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC19911/ https://ncbi.nlm.nih.gov/pubmed/9520441 |
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pubmed-199112001-03-08 Rapid isolation of cDNA by hybridization Hamaguchi, Masaaki O’Connor, Elizabeth A. Chen, Tong Parnell, Larry McCombie, Richard W. Wigler, Michael H. Proc Natl Acad Sci U S A Biological Sciences The isolation of genes from a given genomic region can be a rate-limiting step in the discovery of disease genes. We describe an approach to the isolation of cDNAs that have sequences in common with large genomic clones such as bacterial artificial chromosomes. We applied this method to loci both amplified and deleted in cancer, illustrating its usage in the identification of both oncogenes and tumor suppressor genes, respectively. The method, called rapid isolation of cDNAs by hybridization (RICH), depends on solution hybridization, enzymatic modification, and amplification/selection of sequences present in both cDNA populations and the genomic clones. The method should facilitate the development of transcription maps for large genomic clones, possibly even yeast artificial chromosomes. The National Academy of Sciences 1998-03-31 /pmc/articles/PMC19911/ /pubmed/9520441 Text en Copyright © 1998, The National Academy of Sciences |
| institution |
US National Library of Medicine |
| collection |
PubMed Central |
| language |
en |
| format |
Article |
| topic |
Biological Sciences |
| spellingShingle |
Biological Sciences Hamaguchi, Masaaki O’Connor, Elizabeth A. Chen, Tong Parnell, Larry McCombie, Richard W. Wigler, Michael H. Rapid isolation of cDNA by hybridization |
| description |
The isolation of genes from a given genomic region can be a rate-limiting step in the discovery of disease genes. We describe an approach to the isolation of cDNAs that have sequences in common with large genomic clones such as bacterial artificial chromosomes. We applied this method to loci both amplified and deleted in cancer, illustrating its usage in the identification of both oncogenes and tumor suppressor genes, respectively. The method, called rapid isolation of cDNAs by hybridization (RICH), depends on solution hybridization, enzymatic modification, and amplification/selection of sequences present in both cDNA populations and the genomic clones. The method should facilitate the development of transcription maps for large genomic clones, possibly even yeast artificial chromosomes. |
| author |
Hamaguchi, Masaaki O’Connor, Elizabeth A. Chen, Tong Parnell, Larry McCombie, Richard W. Wigler, Michael H. |
| author_facet |
Hamaguchi, Masaaki O’Connor, Elizabeth A. Chen, Tong Parnell, Larry McCombie, Richard W. Wigler, Michael H. |
| author_sort |
Hamaguchi, Masaaki |
| title |
Rapid isolation of cDNA by hybridization |
| title_short |
Rapid isolation of cDNA by hybridization |
| title_full |
Rapid isolation of cDNA by hybridization |
| title_fullStr |
Rapid isolation of cDNA by hybridization |
| title_full_unstemmed |
Rapid isolation of cDNA by hybridization |
| title_sort |
rapid isolation of cdna by hybridization |
| publisher |
The National Academy of Sciences |
| publisher_facet |
The National Academy of Sciences |
| publishDate |
1998 |
| url |
https://ncbi.nlm.nih.gov/pmc/articles/PMC19911/ https://ncbi.nlm.nih.gov/pubmed/9520441 |
| _version_ |
1758625837170556928 |