RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB

One activity that controls mRNA translation in vertebrate oocytes, embryos, and neurons is cytoplasmic polyadenylation. In Xenopus oocytes, where much of the biochemistry of this process has been elucidated, nuclear pre-mRNAs containing a cytoplasmic polyadenylation element (CPE) in their 3′ untrans...

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Main Authors: Kim, Jong Heon, Richter, Joel D.
Formato: Artigo
Idioma:English
Publicado em: Cold Spring Harbor Laboratory Press 2007
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Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2000322/
https://ncbi.nlm.nih.gov/pubmed/17938241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.1593007
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spelling pubmed-20003222008-04-15 RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB Kim, Jong Heon Richter, Joel D. Genes Dev Research Paper One activity that controls mRNA translation in vertebrate oocytes, embryos, and neurons is cytoplasmic polyadenylation. In Xenopus oocytes, where much of the biochemistry of this process has been elucidated, nuclear pre-mRNAs containing a cytoplasmic polyadenylation element (CPE) in their 3′ untranslated regions (UTRs) have long poly(A) tails; once the RNAs are spliced and transported to the cytoplasm, the tails are shortened. Following the resumption of meiosis, the poly(A) tails are lengthened and translation ensues. CPEB is a sequence-specific RNA-binding protein that coordinates these events and does so by binding to the CPE as well as several factors including Gld2, a poly(A) polymerase, and PARN [poly(A)-specific ribonuclease], a deadenylase. Here, we show that ePAB, embryonic poly(A)-binding protein, transiently associates with the polyadenylation complex; it initially interacts with CPEB, but after polyadenylation, it binds the poly(A) tail. ePAB dissociation from CPEB is regulated by RINGO (Rapid Inducer of G(2)/M progression in Oocytes), a cyclin B1-like cofactor that activates cdk1, a protein kinase that phosphorylates CPEB. Subsequent ePAB binding to the poly(A) tail is necessary to protect the homopolymer from degradation by deadenylating enzymes. Poly(A)-bound ePAB also interacts with eIF4G, which instigates translation initiation of CPEB-bound mRNAs. Cold Spring Harbor Laboratory Press 2007-10-15 /pmc/articles/PMC2000322/ /pubmed/17938241 http://dx.doi.org/10.1101/gad.1593007 Text en Copyright © 2007, Cold Spring Harbor Laboratory Press
institution US National Library of Medicine
collection PubMed Central
language English
format Article
topic Research Paper
spellingShingle Research Paper
Kim, Jong Heon
Richter, Joel D.
RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB
description One activity that controls mRNA translation in vertebrate oocytes, embryos, and neurons is cytoplasmic polyadenylation. In Xenopus oocytes, where much of the biochemistry of this process has been elucidated, nuclear pre-mRNAs containing a cytoplasmic polyadenylation element (CPE) in their 3′ untranslated regions (UTRs) have long poly(A) tails; once the RNAs are spliced and transported to the cytoplasm, the tails are shortened. Following the resumption of meiosis, the poly(A) tails are lengthened and translation ensues. CPEB is a sequence-specific RNA-binding protein that coordinates these events and does so by binding to the CPE as well as several factors including Gld2, a poly(A) polymerase, and PARN [poly(A)-specific ribonuclease], a deadenylase. Here, we show that ePAB, embryonic poly(A)-binding protein, transiently associates with the polyadenylation complex; it initially interacts with CPEB, but after polyadenylation, it binds the poly(A) tail. ePAB dissociation from CPEB is regulated by RINGO (Rapid Inducer of G(2)/M progression in Oocytes), a cyclin B1-like cofactor that activates cdk1, a protein kinase that phosphorylates CPEB. Subsequent ePAB binding to the poly(A) tail is necessary to protect the homopolymer from degradation by deadenylating enzymes. Poly(A)-bound ePAB also interacts with eIF4G, which instigates translation initiation of CPEB-bound mRNAs.
author Kim, Jong Heon
Richter, Joel D.
author_facet Kim, Jong Heon
Richter, Joel D.
author_sort Kim, Jong Heon
title RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB
title_short RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB
title_full RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB
title_fullStr RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB
title_full_unstemmed RINGO/cdk1 and CPEB mediate poly(A) tail stabilization and translational regulation by ePAB
title_sort ringo/cdk1 and cpeb mediate poly(a) tail stabilization and translational regulation by epab
publisher Cold Spring Harbor Laboratory Press
publisher_facet Cold Spring Harbor Laboratory Press
publishDate 2007
url https://ncbi.nlm.nih.gov/pmc/articles/PMC2000322/
https://ncbi.nlm.nih.gov/pubmed/17938241
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gad.1593007
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